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BMRB: 19870

2MMX

Solution Structure of 6aJL2 Amyloidogenic Light Chain Protein

Authors

Amero, C., Maya, R.C., Gil, P.C.

Assembly

6aJL2 Amyloidogenic Light Chain Protein

Entity

1. 6aJL2 Amyloidogenic Light Chain Protein (polymer, Thiol state: all disulfide bound), 111 monomers, 11952.83 Da Detail

NFMLTQPHSV SESPGKTVTI SCTRSSGSIA SNYVQWYQQR PGSSPTTVIY EDNQRPSGVP DRFSGSIDSS SNSASLTISG LKTEDEADYY CQSYDSSNHV VFGGGTKLTV L

Formula weight

11952.83 Da

Entity Connection

disulfide 1 Detail

ID	Type	Value order	Atom ID 1	Atom ID 2
1	disulfide	sing	1:CYS22:SG	1:CYS91:SG

Source organism

Homo sapiens

Exptl. method

solution NMR

Refine. method

simulated annealing

Data set

assigned_chemical_shifts, conformer_family_coord_set

Chem. Shift Complete

Sequence coverage: 96.4 %, Completeness: 85.5 %, Completeness (bb): 93.0 % Detail

Polymer type: polypeptide(L)

	Total	¹H	¹³C	¹⁵N
All	85.5 % (1031 of 1206)	86.9 % (539 of 620)	82.9 % (389 of 469)	88.0 % (103 of 117)
Backbone	93.0 % (608 of 654)	92.0 % (207 of 225)	94.8 % (307 of 324)	89.5 % (94 of 105)
Sidechain	77.7 % (508 of 654)	82.8 % (327 of 395)	70.0 % (173 of 247)	66.7 % (8 of 12)
Aromatic	43.9 % (43 of 98)	87.8 % (43 of 49)	0.0 % (0 of 48)	0.0 % (0 of 1)
Methyl	95.9 % (94 of 98)	95.9 % (47 of 49)	95.9 % (47 of 49)

1. 6aJL2

NFMLTQPHSV SESPGKTVTI SCTRSSGSIA SNYVQWYQQR PGSSPTTVIY EDNQRPSGVP DRFSGSIDSS SNSASLTISG LKTEDEADYY CQSYDSSNHV VFGGGTKLTV L

Show sample condition

Sample #1

Solvent system 95% H2O/5% D2O, Pressure 1 atm, Temperature 298 (±0.1) K, pH 7.4 (±0.1)

#	Name	Isotope labeling	Type	Concentration
1	sodium phosphate	natural abundance	buffer	50 mM
2	sodium chloride	natural abundance	salt	75 mM
3	D2O		solvent	5 %
4	6aJL2	[U-100% 15N]	protein	1 mM
5	H2O		solvent	95 %

Sample #2

Solvent system 95% H2O/5% D2O, Pressure 1 atm, Temperature 298 (±0.1) K, pH 7.4 (±0.1)

#	Name	Isotope labeling	Type	Concentration
6	sodium phosphate	natural abundance	buffer	50 mM
7	sodium chloride	natural abundance	salt	75 mM
8	D2O		solvent	5 %
9	6aJL2	[U-100% 13C; U-100% 15N]	protein	1 mM
10	H2O		solvent	95 %

LACS Plot; CA

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹H	DSS	methyl protons	external	direct	1.0
¹³C	DSS	methyl protons	na	indirect	0.2514495
¹⁵N	DSS	methyl protons	na	indirect	0.1013291

Referencing offset: 0.3 ppm, Outliers: 2 Detail

LACS Plot; CB

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹H	DSS	methyl protons	external	direct	1.0
¹³C	DSS	methyl protons	na	indirect	0.2514495
¹⁵N	DSS	methyl protons	na	indirect	0.1013291

Referencing offset: 0.3 ppm, Outliers: 2 Detail

LACS Plot; HA

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹H	DSS	methyl protons	external	direct	1.0
¹³C	DSS	methyl protons	na	indirect	0.2514495
¹⁵N	DSS	methyl protons	na	indirect	0.1013291

Referencing offset: -0.12 ppm, Outliers: 1 Detail

LACS Plot; CO

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹H	DSS	methyl protons	external	direct	1.0
¹³C	DSS	methyl protons	na	indirect	0.2514495
¹⁵N	DSS	methyl protons	na	indirect	0.1013291

Referencing offset: 0.9 ppm, Outliers: 1 Detail

Protein Blocks Logo

Calculated from 20 models in PDB: 2MMX, Strand ID: A Detail

Release date

2014-06-22

Citation

Solution structure of 6aJL2 and 6aJL2-R24G amyloidogenics light chain proteins
Maya, R.C., Gil, P.C., Amero, C.C.
Biochem. Biophys. Res. Commun. (2015), 456, 695-699, PubMed 25522882 , DOI 10.1016/j.bbrc.2014.12.044 , Abstract

Related entities 1. 6aJL2 Amyloidogenic Light Chain Protein, : 1 : 1 : 6 : 412 entities Detail

Experiments performed 11 experiments Detail

1 2D 1H-15N HSQC