BMRB: 19887

oligonucleotide model of miR-21 pre-element

Authors

Chirayil, S., Wu, Q., Amezcua, C., Luebke, K.

Assembly

Oligonucleotide Model of the MiR-21 Pre-Element

Entity

1. Oligonucleotide Model of the MiR-21 Pre-Element (polymer, Thiol state: not present), 29 monomers, 9338.525 Da Detail

GGGUUGACCG UUGAAUCUCA CGGCAACCC

Formula weight

9338.525 Da

Source organism

Homo sapiens

Exptl. method

solution NMR

Data set

assigned_chemical_shifts

Chem. Shift Complete

Sequence coverage: 55.2 %, Completeness: 36.7 %, Completeness (bb): 34.5 % Detail

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Polymer type: polyribonucleotide

	Total	1H
All	36.7 % (94 of 256)	36.7 % (94 of 256)
Suger, PO4	34.5 % (60 of 174)	34.5 % (60 of 174)
Nucleobase	41.5 % (34 of 82)	41.5 % (34 of 82)
Aromatic	53.1 % (34 of 64)	53.1 % (34 of 64)

1. miR-21 pre-element

GGGUUGACCG UUGAAUCUCA CGGCAACCC

Show sample condition

Sample

Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 273 K, pH 6.7

#	Name	Isotope labeling	Type	Concentration
1	miR-21 pre-element	natural abundance		1 mM
2	H2O	natural abundance		90 %
3	D2O	natural abundance		10 %

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Release date

2014-09-28

Citation

NMR characterization of an oligonucleotide model of the miR-21 pre-element
Chirayil, S., Wu, Q., Amezcua, C., Luebke, K.
PLoS One (2014), 9, e108231-e108231, PubMed 25250627 , DOI 10.1371/journal.pone.0108231 , Abstract

Related entities 1. Oligonucleotide Model of the MiR-21 Pre-Element, : 1 : 2 entities Detail

Experiments performed 3 experiments Detail