BMRB: 19890

MINOR GROOVE RECOGNITION OF DNA BY THIAZOTROPSIN ANALOGUES

Authors

Alniss, H.Y., Salvia, M.-V., Sadikov, M., Golovchenko, I., Anthony, N.G., Khalaf, A.I., Mackay, S.P., Suckling, C.J., Parkinson, J.A.

Assembly

CGACTAGTCG dimer

Entity

1. CGACTAGTCG dimer (polymer), 10 monomers, 3107.976 × 2 Da Detail

CGACTAGTCG

Total weight

6215.952 Da

Max. entity weight

3107.976 Da

Exptl. method

solution NMR

Data set

assigned_chemical_shifts

Chem. Shift Complete

Sequence coverage: 100.0 %, Completeness: 79.6 %, Completeness (bb): 75.7 % Detail

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Polymer type: polydeoxyribonucleotide

	Total	1H
All	79.6 % (78 of 98)	79.6 % (78 of 98)
Suger, PO4	75.7 % (53 of 70)	75.7 % (53 of 70)
Nucleobase	89.3 % (25 of 28)	89.3 % (25 of 28)
Aromatic	95.0 % (19 of 20)	95.0 % (19 of 20)
Methyl	100.0 % (2 of 2)	100.0 % (2 of 2)

1. 5'-D(*CP*GP*AP*CP*TP*AP*GP*TP*CP*G)-3'

CGACTAGTCG

Show sample condition

Sample

Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 298 K, pH 7.4

#	Name	Isotope labeling	Type	Concentration
1	5'-D(*CP*GP*AP*CP*TP*AP*GP*TP*CP*G)-3'	natural abundance		2 mM
2	H2O	natural abundance		90 %
3	D2O	natural abundance		10 %

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Release date

2014-07-16

Citation

Recognition of the DNA minor groove by thiazotropsin analogues
Alniss, H.Y., Salvia, M.-V., Sadikov, M., Golovchenko, I., Anthony, N.G., Khalaf, A.I., Mackay, S.P., Suckling, C.J., Parkinson, J.A.
Chembiochem. (2014), 15, 1978-1990, PubMed 25045155 , DOI 10.1002/cbic.201402202 , Abstract

Experiments performed 3 experiments Detail