BMRBj - BMREntryMaster

	Total	¹H	¹³C	¹⁵N
All	64.0 % (966 of 1509)	63.3 % (494 of 780)	63.7 % (380 of 597)	69.7 % (92 of 132)
Backbone	71.9 % (564 of 784)	71.1 % (194 of 273)	72.2 % (278 of 385)	73.0 % (92 of 126)
Sidechain	58.3 % (492 of 844)	59.2 % (300 of 507)	58.0 % (192 of 331)	0.0 % (0 of 6)
Aromatic	45.9 % (68 of 148)	64.9 % (48 of 74)	27.0 % (20 of 74)
Methyl	71.1 % (91 of 128)	57.8 % (37 of 64)	84.4 % (54 of 64)

1. P22S mutant of human Shq1 CS domain

GSTAIGMKET AAAKFERQHM DSPDLGTGGG SGDDDDKMLT PAFDLSQDPD FLTIAIRVSY ARVSEFDVYF EGSDFKFYAK PYFLRLTLPG RIVENGSEQG SYDADKGIFT IRLPKETPGQ HFEGLNMLTA LLA

Solvent system 95% H2O/5% D2O, Pressure 1 atm, Temperature 298 K, pH 7, Details 15N labeled sample

#	Name	Isotope labeling	Concentration
1	P22S mutant of human Shq1 CS domain	[U-15N]	0.5 ~ 1.0 mM
2	sodium phosphate	natural abundance	20 mM
3	sodium chloride	natural abundance	100 mM
4	DTT	natural abundance	1 mM
5	H2O	natural abundance	95 %
6	D2O	natural abundance	5 %

Solvent system 95% H2O/5% D2O, Pressure 1 atm, Temperature 298 K, pH 7, Details 13C, 15N labeled sample

#	Name	Isotope labeling	Concentration
7	P22S mutant of human Shq1 CS domain	[U-99% 13C; U-99% 15N]	0.5 ~ 1.0 mM
8	sodium phosphate	natural abundance	20 mM
9	sodium chloride	natural abundance	100 mM
10	DTT	natural abundance	1 mM
11	H2O	natural abundance	95 %
12	D2O	natural abundance	5 %

Solvent system 100% D2O, Pressure 1 atm, Temperature 298 K, pH 7, Details 13C, 15N D2O sample

#	Name	Isotope labeling	Concentration
13	P22S mutant of human Shq1 CS domain	[U-99% 13C; U-99% 15N]	0.5 ~ 1.0 mM
14	sodium phosphate	natural abundance	20 mM
15	sodium chloride	natural abundance	100 mM
16	DTT	natural abundance	1 mM
17	D2O	natural abundance	100 %

LACS Plot; CA

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹H	DSS	methyl protons	internal	direct	1.0
¹³C	DSS	methyl protons	na	indirect	0.2514495
¹⁵N	DSS	methyl protons	na	indirect	0.1013291

Referencing offset: -0.01 ppm, Outliers: 1 Detail

LACS Plot; CB

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹H	DSS	methyl protons	internal	direct	1.0
¹³C	DSS	methyl protons	na	indirect	0.2514495
¹⁵N	DSS	methyl protons	na	indirect	0.1013291

Referencing offset: -0.01 ppm, Outliers: 1 Detail

LACS Plot; HA

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹H	DSS	methyl protons	internal	direct	1.0
¹³C	DSS	methyl protons	na	indirect	0.2514495
¹⁵N	DSS	methyl protons	na	indirect	0.1013291

Referencing offset: 0.04 ppm, Outliers: 4 Detail

LACS Plot; CO

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹H	DSS	methyl protons	internal	direct	1.0
¹³C	DSS	methyl protons	na	indirect	0.2514495
¹⁵N	DSS	methyl protons	na	indirect	0.1013291

Referencing offset: 0.7 ppm, Outliers: 3 Detail

Protein Blocks Logo

Calculated from 20 models in PDB: 2MNW, Strand ID: A Detail

Release date

2015-01-11

Citation

Structure and interactions of the CS domain of human H/ACA RNP assembly protein Shq1
Singh, M., Wang, Z., Cascio, D., Feigon, J.
J. Mol. Biol. (2015), 427, 807-823, PubMed 25553844 , DOI 10.1016/j.jmb.2014.12.012 , Abstract