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BMRB: 19915

2MO0

Backbone 1H, 13C, and 15N Chemical Shift Assignments for cold shock protein, TaCsp

Authors

Jeong, K., Kim, Y.

Assembly

cold shock protein, TaCsp

Entity

1. cold shock protein, TaCsp (polymer, Thiol state: not present), 68 monomers, 7679.549 Da Detail

MKKGTVKWFN AEKGYGFIQQ EEGPDVFVHF TAIEADGFRT LNEGEHVEFE VEPGRGGKGP QAKKVRRI

Formula weight

7679.549 Da

Source organism

Thermus aquaticus

Exptl. method

Refine. method

distance geometry

Data set

assigned_chemical_shifts

Chem. Shift Complete

Sequence coverage: 95.6 %, Completeness: 69.7 %, Completeness (bb): 78.4 % Detail

Polymer type: polypeptide(L)

	Total	¹H	¹³C	¹⁵N
All	69.7 % (566 of 812)	79.9 % (342 of 428)	50.5 % (158 of 313)	93.0 % (66 of 71)
Backbone	78.4 % (315 of 402)	95.8 % (137 of 143)	60.8 % (118 of 194)	92.3 % (60 of 65)
Sidechain	64.7 % (303 of 468)	71.9 % (205 of 285)	52.0 % (92 of 177)	100.0 % (6 of 6)
Aromatic	25.0 % (22 of 88)	47.7 % (21 of 44)	0.0 % (0 of 43)	100.0 % (1 of 1)
Methyl	72.2 % (39 of 54)	70.4 % (19 of 27)	74.1 % (20 of 27)

1. entity

MKKGTVKWFN AEKGYGFIQQ EEGPDVFVHF TAIEADGFRT LNEGEHVEFE VEPGRGGKGP QAKKVRRI

Show sample condition

Sample

Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 298 K, pH 6

#	Name	Isotope labeling	Type	Concentration
1	potassium phosphate	natural abundance		50 mM
2	KCl	natural abundance		100 mM
3	EDTA	natural abundance		0.1 mM
4	Thermus aquaticus cold shock protein	[U-99% 13C; U-99% 15N]		0.8 mM
5	H2O	natural abundance		90 %
6	D2O	natural abundance		10 %

LACS Plot; CA

Referencing offset: -1.34 ppm, Outliers: 2 Detail

LACS Plot; CB

Referencing offset: -1.34 ppm, Outliers: 2 Detail

LACS Plot; HA

Referencing offset: -0.06 ppm, Outliers: 2 Detail

Protein Blocks Logo

Calculated from 20 models in PDB: 2MO0, Strand ID: A Detail

Release date

2014-08-24

Citation

Structure and flexibility of the thermophilic cold-shock protein of Thermus aquaticus
Jin, B., Jeong, K.W., Kim, Y.
Biochem. Biophys. Res. Commun. (2014), 451, 402-407, PubMed 25101648 , DOI 10.1016/j.bbrc.2014.07.127 , Abstract

Related entities 1. cold shock protein, TaCsp, : 1 : 1 : 164 entities Detail

Experiments performed 10 experiments Detail

1 3D HNCO

Instrument

Bruker Avance - 800 MHz

Sample

State isotropic, Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 298 K, pH 6

#	Name	Isotope labeling	Type	Concentration
1	potassium phosphate	natural abundance		50 mM
2	KCl	natural abundance		100 mM
3	EDTA	natural abundance		0.1 mM
4	Thermus aquaticus cold shock protein	[U-99% 13C; U-99% 15N]		0.8 mM
5	H2O	natural abundance		90 %
6	D2O	natural abundance		10 %

2 3D HN(CA)CO

Instrument

Bruker Avance - 800 MHz

Sample

State isotropic, Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 298 K, pH 6

#	Name	Isotope labeling	Type	Concentration
1	potassium phosphate	natural abundance		50 mM
2	KCl	natural abundance		100 mM
3	EDTA	natural abundance		0.1 mM
4	Thermus aquaticus cold shock protein	[U-99% 13C; U-99% 15N]		0.8 mM
5	H2O	natural abundance		90 %
6	D2O	natural abundance		10 %

3 3D CBCA(CO)NH

Instrument

Bruker Avance - 800 MHz

Sample

State isotropic, Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 298 K, pH 6

#	Name	Isotope labeling	Type	Concentration
1	potassium phosphate	natural abundance		50 mM
2	KCl	natural abundance		100 mM
3	EDTA	natural abundance		0.1 mM
4	Thermus aquaticus cold shock protein	[U-99% 13C; U-99% 15N]		0.8 mM
5	H2O	natural abundance		90 %
6	D2O	natural abundance		10 %

4 3D HN(CO)CA

Instrument

Bruker Avance - 800 MHz

Sample

State isotropic, Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 298 K, pH 6

#	Name	Isotope labeling	Type	Concentration
1	potassium phosphate	natural abundance		50 mM
2	KCl	natural abundance		100 mM
3	EDTA	natural abundance		0.1 mM
4	Thermus aquaticus cold shock protein	[U-99% 13C; U-99% 15N]		0.8 mM
5	H2O	natural abundance		90 %
6	D2O	natural abundance		10 %

5 3D HBHA(CO)NH

Instrument

Bruker Avance - 800 MHz

Sample

State isotropic, Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 298 K, pH 6

#	Name	Isotope labeling	Type	Concentration
1	potassium phosphate	natural abundance		50 mM
2	KCl	natural abundance		100 mM
3	EDTA	natural abundance		0.1 mM
4	Thermus aquaticus cold shock protein	[U-99% 13C; U-99% 15N]		0.8 mM
5	H2O	natural abundance		90 %
6	D2O	natural abundance		10 %

6 3D HNHA

Instrument

Bruker Avance - 800 MHz

Sample

State isotropic, Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 298 K, pH 6

#	Name	Isotope labeling	Type	Concentration
1	potassium phosphate	natural abundance		50 mM
2	KCl	natural abundance		100 mM
3	EDTA	natural abundance		0.1 mM
4	Thermus aquaticus cold shock protein	[U-99% 13C; U-99% 15N]		0.8 mM
5	H2O	natural abundance		90 %
6	D2O	natural abundance		10 %

7 3D HCCH-TOCSY

Instrument

Bruker Avance - 800 MHz

Sample

State isotropic, Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 298 K, pH 6

#	Name	Isotope labeling	Type	Concentration
1	potassium phosphate	natural abundance		50 mM
2	KCl	natural abundance		100 mM
3	EDTA	natural abundance		0.1 mM
4	Thermus aquaticus cold shock protein	[U-99% 13C; U-99% 15N]		0.8 mM
5	H2O	natural abundance		90 %
6	D2O	natural abundance		10 %

8 3D 1H-15N NOESY

Instrument

Bruker Avance - 800 MHz

Sample

State isotropic, Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 298 K, pH 6

#	Name	Isotope labeling	Type	Concentration
1	potassium phosphate	natural abundance		50 mM
2	KCl	natural abundance		100 mM
3	EDTA	natural abundance		0.1 mM
4	Thermus aquaticus cold shock protein	[U-99% 13C; U-99% 15N]		0.8 mM
5	H2O	natural abundance		90 %
6	D2O	natural abundance		10 %

9 3D 1H-13C NOESY

Instrument

Bruker Avance - 800 MHz

Sample

State isotropic, Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 298 K, pH 6

#	Name	Isotope labeling	Type	Concentration
1	potassium phosphate	natural abundance		50 mM
2	KCl	natural abundance		100 mM
3	EDTA	natural abundance		0.1 mM
4	Thermus aquaticus cold shock protein	[U-99% 13C; U-99% 15N]		0.8 mM
5	H2O	natural abundance		90 %
6	D2O	natural abundance		10 %

10 2D 1H-15N HSQC

Instrument

Bruker Avance - 800 MHz

Sample

State isotropic, Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 298 K, pH 6

#	Name	Isotope labeling	Type	Concentration
1	potassium phosphate	natural abundance		50 mM
2	KCl	natural abundance		100 mM
3	EDTA	natural abundance		0.1 mM
4	Thermus aquaticus cold shock protein	[U-99% 13C; U-99% 15N]		0.8 mM
5	H2O	natural abundance		90 %
6	D2O	natural abundance		10 %

nullKeywords cold shock protein, nuclear magnetic resonance, protein binding, protien stability, thermus aquaticus