Solution NMR structure of DNA dodecamer containing the 5-hydroxycytosine
Polymer type: polydeoxyribonucleotide
Total | 1H | |
---|---|---|
All | 57.9 % (62 of 107) | 57.9 % (62 of 107) |
Suger, PO4 | 57.1 % (44 of 77) | 57.1 % (44 of 77) |
Nucleobase | 60.0 % (18 of 30) | 60.0 % (18 of 30) |
Aromatic | 72.7 % (16 of 22) | 72.7 % (16 of 22) |
Methyl | 100.0 % (2 of 2) | 100.0 % (2 of 2) |
1. DNA (5'-D(*CP*GP*CP*GP*AP*AP*TP*TP*(OHC)P*GP*CP*G)-3')
CGCGAATTXG CGSolvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 288 K, pH 7
# | Name | Isotope labeling | Type | Concentration |
---|---|---|---|---|
1 | DNA (5'-D(*CP*GP*CP*GP*AP*AP*TP*TP*(OHC)P*GP*CP*G)-3') | natural abundance | 100 mM | |
2 | D2O | natural abundance | 10 % | |
3 | sodium phosphate | natural abundance | 10 mM | |
4 | sodium chloride | natural abundance | 100 mM | |
5 | sodium azide | natural abundance | 11 mM | |
6 | EDTA | natural abundance | 0.05 mM | |
7 | H2O | natural abundance | 90 % |