3D NMR structure of the cytoplasmic rhodanese domain of the inner membrane protein YgaP from Escherichia coli
ALTTISPHDA QELIARGAKL IDIRDADEYL REHIPEADLA PLSVLEQSGL PAKLRHEQII FHCQAGKRTS NNADKLAAIA APAEIFLLED GIDGWKKAGL PVAVNKSQ
Polymer type: polypeptide(L)
Total | 1H | 13C | 15N | |
---|---|---|---|---|
All | 81.9 % (1004 of 1226) | 87.6 % (557 of 636) | 70.8 % (339 of 479) | 97.3 % (108 of 111) |
Backbone | 82.5 % (525 of 636) | 96.3 % (208 of 216) | 68.2 % (217 of 318) | 98.0 % (100 of 102) |
Sidechain | 82.7 % (572 of 692) | 83.1 % (349 of 420) | 81.7 % (215 of 263) | 88.9 % (8 of 9) |
Aromatic | 53.6 % (30 of 56) | 60.7 % (17 of 28) | 44.4 % (12 of 27) | 100.0 % (1 of 1) |
Methyl | 97.9 % (141 of 144) | 98.6 % (71 of 72) | 97.2 % (70 of 72) |
1. YgaP
ALTTISPHDA QELIARGAKL IDIRDADEYL REHIPEADLA PLSVLEQSGL PAKLRHEQII FHCQAGKRTS NNADKLAAIA APAEIFLLED GIDGWKKAGL PVAVNKSQSolvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 273 K, pH 7.0
# | Name | Isotope labeling | Type | Concentration |
---|---|---|---|---|
1 | YgaP | [U-100% 13C; U-100% 15N] | 2 mM | |
2 | H2O | natural abundance | 90 % | |
3 | D2O | [U-100% 2H] | 10 % |