Assignment of DNA-MC1 protein complex
SNTRNFVLRD EDGNEHGVFT GKQPRQAALK AANRGSGTKA NPDIIRLRER GTKKVHVFKA WKEIVDAPKN RPAWMPEKIS KPFVKKERIE KLE
Polymer type: polypeptide(L) polydeoxyribonucleotide
Total | 1H | 13C | 15N | |
---|---|---|---|---|
All | 44.9 % (640 of 1426) | 58.1 % (522 of 898) | 6.7 % (29 of 431) | 91.8 % (89 of 97) |
Backbone | 38.1 % (288 of 756) | 45.5 % (180 of 396) | 10.3 % (28 of 273) | 92.0 % (80 of 87) |
Sidechain | 47.4 % (359 of 757) | 68.1 % (342 of 502) | 3.3 % (8 of 245) | 90.0 % (9 of 10) |
Aromatic | 28.0 % (37 of 132) | 36.5 % (35 of 96) | 0.0 % (0 of 34) | 100.0 % (2 of 2) |
Methyl | 45.2 % (42 of 93) | 82.4 % (42 of 51) | 0.0 % (0 of 42) |
1. MC1
SNTRNFVLRD EDGNEHGVFT GKQPRQAALK AANRGSGTKA NPDIIRLRER GTKKVHVFKA WKEIVDAPKN RPAWMPEKIS KPFVKKERIE KLE2. DNA1
AAAAACACAC ACCCA3. DNA2
TGGGTGTGTG TTTTTSolvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 299 K, pH 6
# | Name | Isotope labeling | Type | Concentration |
---|---|---|---|---|
1 | MC1 | [U-15N] | 1.18 mM | |
2 | DNA1 | [U-13C; U-15N] | 1.18 mM | |
3 | DNA2 | natural abundance | 1.18 mM | |
4 | sodium phosphate | natural abundance | 10 mM | |
5 | sodium chloride | natural abundance | 100 mM | |
6 | EDTA | natural abundance | 1 mM | |
7 | H2O | natural abundance | 90 % | |
8 | D2O | natural abundance | 10 % |
Polymer type: polypeptide(L) polydeoxyribonucleotide
Total | 1H | 13C | 15N | |
---|---|---|---|---|
All | 31.6 % (900 of 2852) | 39.6 % (711 of 1796) | 9.7 % (84 of 862) | 54.1 % (105 of 194) |
Backbone | 30.7 % (464 of 1512) | 37.4 % (296 of 792) | 13.2 % (72 of 546) | 55.2 % (96 of 174) |
Sidechain | 30.3 % (459 of 1514) | 41.3 % (415 of 1004) | 7.1 % (35 of 490) | 45.0 % (9 of 20) |
Aromatic | 25.4 % (67 of 264) | 33.9 % (65 of 192) | 0.0 % (0 of 68) | 50.0 % (2 of 4) |
Methyl | 28.0 % (52 of 186) | 46.1 % (47 of 102) | 6.0 % (5 of 84) |
1. MC1
SNTRNFVLRD EDGNEHGVFT GKQPRQAALK AANRGSGTKA NPDIIRLRER GTKKVHVFKA WKEIVDAPKN RPAWMPEKIS KPFVKKERIE KLE2. DNA1
AAAAACACAC ACCCA3. DNA2
TGGGTGTGTG TTTTTSolvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 299 K, pH 6
# | Name | Isotope labeling | Type | Concentration |
---|---|---|---|---|
1 | MC1 | [U-15N] | 1.18 mM | |
2 | DNA1 | [U-13C; U-15N] | 1.18 mM | |
3 | DNA2 | natural abundance | 1.18 mM | |
4 | sodium phosphate | natural abundance | 10 mM | |
5 | sodium chloride | natural abundance | 100 mM | |
6 | EDTA | natural abundance | 1 mM | |
7 | H2O | natural abundance | 90 % | |
8 | D2O | natural abundance | 10 % |
Polymer type: polypeptide(L) polydeoxyribonucleotide
Total | 1H | 13C | 15N | |
---|---|---|---|---|
All | 24.9 % (1066 of 4278) | 31.4 % (847 of 2694) | 7.8 % (101 of 1293) | 40.5 % (118 of 291) |
Backbone | 25.5 % (579 of 2268) | 32.4 % (385 of 1188) | 10.4 % (85 of 819) | 41.8 % (109 of 261) |
Sidechain | 22.7 % (515 of 2271) | 30.7 % (462 of 1506) | 6.0 % (44 of 735) | 30.0 % (9 of 30) |
Aromatic | 24.2 % (96 of 396) | 32.6 % (94 of 288) | 0.0 % (0 of 102) | 33.3 % (2 of 6) |
Methyl | 25.4 % (71 of 279) | 39.9 % (61 of 153) | 7.9 % (10 of 126) |
1. MC1
SNTRNFVLRD EDGNEHGVFT GKQPRQAALK AANRGSGTKA NPDIIRLRER GTKKVHVFKA WKEIVDAPKN RPAWMPEKIS KPFVKKERIE KLE2. DNA1
AAAAACACAC ACCCA3. DNA2
TGGGTGTGTG TTTTTSolvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 299 K, pH 6
# | Name | Isotope labeling | Type | Concentration |
---|---|---|---|---|
1 | MC1 | [U-15N] | 1.18 mM | |
2 | DNA1 | [U-13C; U-15N] | 1.18 mM | |
3 | DNA2 | natural abundance | 1.18 mM | |
4 | sodium phosphate | natural abundance | 10 mM | |
5 | sodium chloride | natural abundance | 100 mM | |
6 | EDTA | natural abundance | 1 mM | |
7 | H2O | natural abundance | 90 % | |
8 | D2O | natural abundance | 10 % |