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BMRB: 19957

2NBJ

Assignment of DNA-MC1 protein complex

Authors

Loth, K., Landon, C., Paquet, F.

Assembly

DNA-MC1

Entity

1. MC1 (polymer, Thiol state: not present), 93 monomers, 10663.11 Da Detail

SNTRNFVLRD EDGNEHGVFT GKQPRQAALK AANRGSGTKA NPDIIRLRER GTKKVHVFKA WKEIVDAPKN RPAWMPEKIS KPFVKKERIE KLE

2. DNA1 (polymer, Thiol state: not present), 15 monomers, 4571.966 Da Detail

AAAAACACAC ACCCA

3. DNA2 (polymer, Thiol state: not present), 15 monomers, 4730.984 Da Detail

TGGGTGTGTG TTTTT

Total weight

19966.06 Da

Max. entity weight

10663.11 Da

Source organism

Escherichia coli

Exptl. method

solution NMR

Data set

assigned_chemical_shifts

Chem. Shift Complete1

Sequence coverage: 74.8 %, Completeness: 44.9 %, Completeness (bb): 38.1 % Detail

Polymer type: polypeptide(L) polydeoxyribonucleotide

	Total	¹H	¹³C	¹⁵N
All	44.9 % (640 of 1426)	58.1 % (522 of 898)	6.7 % (29 of 431)	91.8 % (89 of 97)
Backbone	38.1 % (288 of 756)	45.5 % (180 of 396)	10.3 % (28 of 273)	92.0 % (80 of 87)
Sidechain	47.4 % (359 of 757)	68.1 % (342 of 502)	3.3 % (8 of 245)	90.0 % (9 of 10)
Aromatic	28.0 % (37 of 132)	36.5 % (35 of 96)	0.0 % (0 of 34)	100.0 % (2 of 2)
Methyl	45.2 % (42 of 93)	82.4 % (42 of 51)	0.0 % (0 of 42)

1. MC1

SNTRNFVLRD EDGNEHGVFT GKQPRQAALK AANRGSGTKA NPDIIRLRER GTKKVHVFKA WKEIVDAPKN RPAWMPEKIS KPFVKKERIE KLE

2. DNA1

AAAAACACAC ACCCA

3. DNA2

TGGGTGTGTG TTTTT

Show sample condition

Sample

Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 299 K, pH 6

#	Name	Isotope labeling	Concentration
1	MC1	[U-15N]	1.18 mM
2	DNA1	[U-13C; U-15N]	1.18 mM
3	DNA2	natural abundance	1.18 mM
4	sodium phosphate	natural abundance	10 mM
5	sodium chloride	natural abundance	100 mM
6	EDTA	natural abundance	1 mM
7	H2O	natural abundance	90 %
8	D2O	natural abundance	10 %

Chem. Shift Complete2

Sequence coverage: 27.6 %, Completeness: 31.6 %, Completeness (bb): 30.7 % Detail

Polymer type: polypeptide(L) polydeoxyribonucleotide

	Total	¹H	¹³C	¹⁵N
All	31.6 % (900 of 2852)	39.6 % (711 of 1796)	9.7 % (84 of 862)	54.1 % (105 of 194)
Backbone	30.7 % (464 of 1512)	37.4 % (296 of 792)	13.2 % (72 of 546)	55.2 % (96 of 174)
Sidechain	30.3 % (459 of 1514)	41.3 % (415 of 1004)	7.1 % (35 of 490)	45.0 % (9 of 20)
Aromatic	25.4 % (67 of 264)	33.9 % (65 of 192)	0.0 % (0 of 68)	50.0 % (2 of 4)
Methyl	28.0 % (52 of 186)	46.1 % (47 of 102)	6.0 % (5 of 84)

1. MC1

SNTRNFVLRD EDGNEHGVFT GKQPRQAALK AANRGSGTKA NPDIIRLRER GTKKVHVFKA WKEIVDAPKN RPAWMPEKIS KPFVKKERIE KLE

2. DNA1

AAAAACACAC ACCCA

3. DNA2

TGGGTGTGTG TTTTT

Show sample condition

Sample

Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 299 K, pH 6

#	Name	Isotope labeling	Concentration
1	MC1	[U-15N]	1.18 mM
2	DNA1	[U-13C; U-15N]	1.18 mM
3	DNA2	natural abundance	1.18 mM
4	sodium phosphate	natural abundance	10 mM
5	sodium chloride	natural abundance	100 mM
6	EDTA	natural abundance	1 mM
7	H2O	natural abundance	90 %
8	D2O	natural abundance	10 %

Chem. Shift Complete3

Sequence coverage: 23.6 %, Completeness: 24.9 %, Completeness (bb): 25.5 % Detail

Polymer type: polypeptide(L) polydeoxyribonucleotide

	Total	¹H	¹³C	¹⁵N
All	24.9 % (1066 of 4278)	31.4 % (847 of 2694)	7.8 % (101 of 1293)	40.5 % (118 of 291)
Backbone	25.5 % (579 of 2268)	32.4 % (385 of 1188)	10.4 % (85 of 819)	41.8 % (109 of 261)
Sidechain	22.7 % (515 of 2271)	30.7 % (462 of 1506)	6.0 % (44 of 735)	30.0 % (9 of 30)
Aromatic	24.2 % (96 of 396)	32.6 % (94 of 288)	0.0 % (0 of 102)	33.3 % (2 of 6)
Methyl	25.4 % (71 of 279)	39.9 % (61 of 153)	7.9 % (10 of 126)

1. MC1

SNTRNFVLRD EDGNEHGVFT GKQPRQAALK AANRGSGTKA NPDIIRLRER GTKKVHVFKA WKEIVDAPKN RPAWMPEKIS KPFVKKERIE KLE

2. DNA1

AAAAACACAC ACCCA

3. DNA2

TGGGTGTGTG TTTTT

Show sample condition

Sample

Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 299 K, pH 6

#	Name	Isotope labeling	Concentration
1	MC1	[U-15N]	1.18 mM
2	DNA1	[U-13C; U-15N]	1.18 mM
3	DNA2	natural abundance	1.18 mM
4	sodium phosphate	natural abundance	10 mM
5	sodium chloride	natural abundance	100 mM
6	EDTA	natural abundance	1 mM
7	H2O	natural abundance	90 %
8	D2O	natural abundance	10 %

Protein Blocks Logo

Calculated from 12 models in PDB: 2NBJ, Strand ID: A Detail

Release date

2014-10-13

Citation

Chemical shifts assignments of the archaeal MC1 protein and a strongly bent 15 base pairs DNA duplex in complex
Loth, K., Landon, C., Paquet, F.
Biomol. NMR Assign. (2015), 9, 215-217, PubMed 25212183 , DOI 10.1007/s12104-014-9577-8 , Abstract

Related entities 1. MC1, : 1 : 3 : 7 entities Detail

Experiments performed 6 experiments Detail

1 2D 1H-15N HSQC