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BMRB: 19971

2MXA

CupS

Authors

Korste, A., Wulfhorst, H., Ikegami, T., Nowaczyk, M.M., Stoll, R.

Assembly

CupS

Entity

1. CupS (polymer, Thiol state: not present), 159 monomers, 16970.24 Da Detail

MATIVDIAVN TPGFSTLVTA VKVANLVEAL QSPGPFTVFA PNDDAFAKLP DGTITSLVQN PPQLGRILKY HVVAGAYKAT DLKRMGIVTS LEGSTIPIHG DNPLEVKNAT VLAADIEAEN GIIHVIDTVI LMGLDPAHSF QETNIPYKVS AWSHPQFEK

Formula weight

16970.24 Da

Source organism

Thermosynechococcus elongatus

Exptl. method

solution NMR

Refine. method

torsion angle dynamics

Data set

assigned_chemical_shifts

Chem. Shift Complete

Sequence coverage: 99.4 %, Completeness: 92.4 %, Completeness (bb): 96.6 % Detail

Polymer type: polypeptide(L)

	Total	¹H	¹³C	¹⁵N
All	92.4 % (1656 of 1792)	92.8 % (848 of 914)	90.7 % (650 of 717)	98.1 % (158 of 161)
Backbone	96.6 % (898 of 930)	97.2 % (307 of 316)	95.7 % (447 of 467)	98.0 % (144 of 147)
Sidechain	89.6 % (906 of 1011)	90.5 % (541 of 598)	88.0 % (351 of 399)	100.0 % (14 of 14)
Aromatic	50.0 % (58 of 116)	53.4 % (31 of 58)	45.6 % (26 of 57)	100.0 % (1 of 1)
Methyl	96.5 % (220 of 228)	97.4 % (111 of 114)	95.6 % (109 of 114)

1. CupS

MATIVDIAVN TPGFSTLVTA VKVANLVEAL QSPGPFTVFA PNDDAFAKLP DGTITSLVQN PPQLGRILKY HVVAGAYKAT DLKRMGIVTS LEGSTIPIHG DNPLEVKNAT VLAADIEAEN GIIHVIDTVI LMGLDPAHSF QETNIPYKVS AWSHPQFEK

Show sample condition

Sample

Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 298 K, pH 8.0, Details Buffer: 50 mM Tris (pH 8.0), 50 mM NaCl, 10 mM deuterated DTT

#	Name	Isotope labeling	Concentration
1	CupS	[U-99% 13C; U-99% 15N]	0.5 mM
2	Tris	natural abundance	50 mM
3	NaCl	natural abundance	50 mM
4	DTT	[U-2H]	10 mM

LACS Plot; CA

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
¹³C	water	protons	4.77 ppm	internal	indirect	0.2514684
¹H	water	protons	4.77 ppm	internal	direct	1.0
¹⁵N	water	protons	4.77 ppm	internal	indirect	0.1013402

Referencing offset: -0.26 ppm, Outliers: 4 Detail

LACS Plot; CB

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
¹³C	water	protons	4.77 ppm	internal	indirect	0.2514684
¹H	water	protons	4.77 ppm	internal	direct	1.0
¹⁵N	water	protons	4.77 ppm	internal	indirect	0.1013402

Referencing offset: -0.26 ppm, Outliers: 4 Detail

LACS Plot; HA

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
¹³C	water	protons	4.77 ppm	internal	indirect	0.2514684
¹H	water	protons	4.77 ppm	internal	direct	1.0
¹⁵N	water	protons	4.77 ppm	internal	indirect	0.1013402

Referencing offset: 0.02 ppm, Outliers: 2 Detail

LACS Plot; CO

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
¹³C	water	protons	4.77 ppm	internal	indirect	0.2514684
¹H	water	protons	4.77 ppm	internal	direct	1.0
¹⁵N	water	protons	4.77 ppm	internal	indirect	0.1013402

Referencing offset: 0.35 ppm, Outliers: 1 Detail

Protein Blocks Logo

Calculated from 10 models in PDB: 2MXA, Strand ID: A Detail

Release date

2015-06-07

Citation

Solution structure of the NDH-1 complex subunit CupS from Thermosynechococcus elongatus
Korste, A., Wulfhorst, H., Ikegami, T., Nowaczyk, M.M., Stoll, R.
Biochim. Biophys. Acta (2015), 1847, 1212-1219, PubMed 25982357 , DOI 10.1016/j.bbabio.2015.05.003 , Abstract

Related entities 1. CupS, : 1 : 2 : 46 entities Detail

Experiments performed 13 experiments Detail

1 2D 1H-15N HSQC/HMQC