BMRBj - BMREntryMaster

Found 1 Document (0.797 seconds)

BMRB: 19989

2MPM

Structural Basis of Receptor Sulfotyrosine Recognition by a CC Chemokine: the N-terminal Region of CCR3 Bound to CCL11/Eotaxin-1

Authors

Millard, C.J., Ludeman, J.P., Canals, M., Bridgford, J.L., Hinds, M.G., Clayton, D.J., Christopoulos, A., Payne, R.J., Stone, M.J.

Assembly

N-terminal Region of CCR3 Bound to CCL11/Eotaxin-1

Entity

1. N-terminal Region of CCR3 Bound to CCL11/Eotaxin-1, entity 1 (polymer), 74 monomers, 8364.805 Da Detail

GPASVPTTCC FNLANRKIPL QRLESYRRIT SGKCPQKAVI FKTKLAKDIC ADPKKKWVQD SMKYLDQKSP TPKP

2. N-terminal Region of CCR3 Bound to CCL11/Eotaxin-1, entity 2 (polymer, Thiol state: not present), 16 monomers, 1940.021 Da Detail

VETFGTTSXX DDVGLL

Total weight

10304.826 Da

Max. entity weight

8364.805 Da

Source organism

Homo sapiens

Exptl. method

solution NMR

Refine. method

TORSION ANGLE DYNAMICS

Data set

assigned_chemical_shifts

Chem. Shift Complete

Sequence coverage: 93.3 %, Completeness: 74.3 %, Completeness (bb): 67.4 % Detail

Polymer type: polypeptide(L)

	Total	¹H	¹³C	¹⁵N
All	74.3 % (778 of 1047)	86.1 % (476 of 553)	57.7 % (235 of 407)	77.0 % (67 of 87)
Backbone	67.4 % (345 of 512)	82.6 % (142 of 172)	54.6 % (142 of 260)	76.2 % (61 of 80)
Sidechain	80.6 % (499 of 619)	87.7 % (334 of 381)	68.8 % (159 of 231)	85.7 % (6 of 7)
Aromatic	44.8 % (26 of 58)	86.2 % (25 of 29)	0.0 % (0 of 28)	100.0 % (1 of 1)
Methyl	82.2 % (74 of 90)	95.6 % (43 of 45)	68.9 % (31 of 45)

1. entity 1

GPASVPTTCC FNLANRKIPL QRLESYRRIT SGKCPQKAVI FKTKLAKDIC ADPKKKWVQD SMKYLDQKSP TPKP

2. entity 2

VETFGTTSXX DDVGLL

Show sample condition

Sample

Solvent system 95% H2O/5% D2O, Pressure 1 atm, Temperature 313 (±0.1) K, pH 7.0 (±0.1), Details 0.2-0.4 mM 13C15N CCL11/ Eotaxin-1 in a 1:1 or binary complex with CCR3 Su1617 (residues 8-23) sulfopeptide. 20 mM sodium acetate-d6 (pH 6.5) containing 5% v/v D2O, 0.02% sodium azide as preservative and 20 uM DSS as internal standard.

#	Name	Isotope labeling	Concentration
1	CCL11/Eotaxin-1	[U-100% 13C; U-100% 15N]	0.2 ~ 0.4 mM
2	CCR3 Su1617 sulfopeptide	natural abundance	0.2 ~ 0.4 mM
3	H2O	natural abundance	95 %
4	D2O	[U-100% 2H]	5 %
5	sodium acetate-d6	[U-100% 2H]	20 mM
6	sodium azide	natural abundance	0.02 %
7	DSS	natural abundance	20 uM

Protein Blocks Logo

Calculated from 20 models in PDB: 2MPM, Strand ID: A, B Detail

Release date

2014-12-07

Citation

Structural basis of receptor sulfotyrosine recognition by a CC chemokine: the N-terminal region of CCR3 bound to CCL11/eotaxin-1
Millard, C.J., Ludeman, J.P., Canals, M., Bridgford, J.L., Hinds, M.G., Clayton, D.J., Christopoulos, A., Payne, R.J., Stone, M.J.
Structure (2014), 22, 1571-1581, PubMed 25450766 , DOI 10.1016/j.str.2014.08.023 , Abstract

Related entities 1. N-terminal Region of CCR3 Bound to CCL11/Eotaxin-1, entity 1, : 1 : 83 entities Detail

Related entities 2. N-terminal Region of CCR3 Bound to CCL11/Eotaxin-1, entity 2, : 1 : 2 : 3 entities Detail

Interaction partners 2. N-terminal Region of CCR3 Bound to CCL11/Eotaxin-1, entity 2, : 1 interactors Detail

More details for 1 interactors identified by 1 citations