BMRB: 19994

3D NMR structure of the transmembrane domain of the full-length inner membrane protein YgaP from Escherichia coli

Authors

Eichmann, C., Tzitzilonis, C., Bordignon, E., Maslennikov, I., Choe, S., Riek, R.

Assembly

protein YgaP from Escherichia coli

Entity

1. protein YgaP from Escherichia coli (polymer, Thiol state: not present), 68 monomers, 7152.455 × 2 Da Detail

KSQPLPLMRQ VQIAAGGLIL IGVVLGYTVN SGFFLLSGFV GAGLLFAGIS GFSGMARLLD KMPWNQRA

Total weight

14304.91 Da

Max. entity weight

7152.455 Da

Source organism

Escherichia coli K-12

Exptl. method

solution NMR

Refine. method

simulated annealing

Data set

assigned_chemical_shifts

Chem. Shift Complete

Sequence coverage: 94.1 %, Completeness: 40.9 %, Completeness (bb): 45.5 % Detail

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Polymer type: polypeptide(L)

	Total	1H	13C	15N
All	40.9 % (322 of 788)	57.8 % (234 of 405)	8.7 % (27 of 311)	84.7 % (61 of 72)
Backbone	45.5 % (183 of 402)	77.1 % (111 of 144)	7.3 % (14 of 193)	89.2 % (58 of 65)
Sidechain	32.3 % (143 of 443)	47.1 % (123 of 261)	9.7 % (17 of 175)	42.9 % (3 of 7)
Aromatic	21.4 % (15 of 70)	25.7 % (9 of 35)	14.7 % (5 of 34)	100.0 % (1 of 1)
Methyl	38.3 % (36 of 94)	70.2 % (33 of 47)	6.4 % (3 of 47)

1. entity

KSQPLPLMRQ VQIAAGGLIL IGVVLGYTVN SGFFLLSGFV GAGLLFAGIS GFSGMARLLD KMPWNQRA

Show sample condition

Sample #1

Solvent system 95% H2O/5% D2O, Pressure 1 atm, Temperature 273 K, pH 7.0

#	Name	Isotope labeling	Type	Concentration
1	entity	[U-100% 13C; U-100% 15N; U-80% 2H]		0.5 mM
2	H2O	natural abundance		95 %
3	D2O	natural abundance		5 %

Sample #2

Solvent system 95% H2O/5% D2O, Pressure 1 atm, Temperature 273 K, pH 7.0

#	Name	Isotope labeling	Type	Concentration
4	entity	[U-100% 13C; U-100% 15N]		0.5 mM
5	H2O	natural abundance		95 %
6	D2O	natural abundance		5 %

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Protein Blocks Logo

Calculated from 10 models in PDB: 2MPN, Strand ID: A, B Detail

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Release date

2014-09-09

Citation

Solution NMR structure and functional analysis of the integral membrane protein YgaP from Escherichia coli
Eichmann, C., Tzitzilonis, C., Bordignon, E., Maslennikov, I., Choe, S., Riek, R.
J. Biol. Chem. (2014), 289, 23482-23503, PubMed 24958726 , DOI 10.1074/jbc.M114.571935 , Abstract

Entries sharing articles BMRB: 2 entries Detail

Related entities 1. protein YgaP from Escherichia coli, : 1 : 2 : 1 entities Detail

Interaction partners 1. protein YgaP from Escherichia coli, : 1 interactors Detail

Experiments performed 7 experiments Detail