BMRBj - BMREntryMaster

Found 1 Document (5.144 seconds)

BMRB: 20074

2KIB

Protein Fibril

Authors

Nielsen, J.T., Bjerring, M., Jeppesen, M.D., Pedersen, R.O., Pedersen, J.M., Hein, K.L., Vosegaard, T., Skrydstrup, T., Otzen, D.E., Nielsen, N.

Assembly

octamer

Entity

1. octamer (polymer, Thiol state: not present), 10 monomers, 1009.072 × 8 Da Detail

SNNFGAILSS

Total weight

8072.576 Da

Max. entity weight

1009.072 Da

Exptl. method

solid-state NMR

Refine. method

torsion angle dynamics

Data set

assigned_chemical_shifts

Chem. Shift Complete

Sequence coverage: 60.0 %, Completeness: 65.4 %, Completeness (bb): 59.0 % Detail

Polymer type: polypeptide(L)

	Total	¹³C	¹⁵N
All	65.4 % (34 of 52)	70.0 % (28 of 40)	50.0 % (6 of 12)
Backbone	59.0 % (23 of 39)	58.6 % (17 of 29)	60.0 % (6 of 10)
Sidechain	72.7 % (16 of 22)	80.0 % (16 of 20)	0.0 % (0 of 2)
Aromatic	100.0 % (5 of 5)	100.0 % (5 of 5)
Methyl	100.0 % (5 of 5)	100.0 % (5 of 5)

1. NFGAILchain

SNNFGAILSS

Show sample condition

Sample

Temperature 263 K, pH 7.2

#	Name	Isotope labeling	Type	Concentration
1	NFGAIL	[U-100% 13C; U-100% 15N]		2.5 mg
2	H2O	natural abundance		0.0 ~ 0.0 %
3	HEPES	natural abundance		0.0 ~ 0.0 mM

Protein Blocks Logo

Calculated from 10 models in PDB: 2KIB, Strand ID: A, B, C, D, E, F, G, H Detail

Release date

2009-02-09

Citation

Unique identification of supramolecular structures in amyloid fibrils by solid-state NMR spectroscopy
Nielsen, J.T., Bjerring, M., Jeppesen, M., Pedersen, R.O., Pedersen, J.M., Hein, K.L., Vosegaard, T., Skrydstrup, T., Otzen, D.E., Nielsen, N.
Angew. Chem Int Ed Engl. (2009), 48, 2118-2121, PubMed 19130518 , DOI 10.1002/anie.200804198 , Abstract

Related entities 1. octamer, : 8 : 1 entities Detail

Experiments performed 8 experiments Detail

1 NCA

Instrument

Bruker Avance - 700 MHz 4 mm triple-resonance MAS probe

Sample

State solid, Temperature 263 K, pH 7.2

#	Name	Isotope labeling	Type	Concentration
1	NFGAIL	[U-100% 13C; U-100% 15N]		2.5 mg
2	H2O	natural abundance		0.0 ~ 0.0 %
3	HEPES	natural abundance		0.0 ~ 0.0 mM

2 NCO

Instrument

Bruker Avance - 700 MHz 4 mm triple-resonance MAS probe

Sample

State solid, Temperature 263 K, pH 7.2

#	Name	Isotope labeling	Type	Concentration
1	NFGAIL	[U-100% 13C; U-100% 15N]		2.5 mg
2	H2O	natural abundance		0.0 ~ 0.0 %
3	HEPES	natural abundance		0.0 ~ 0.0 mM

3 2D 13C-13C DARR 2.5 ms mixing

Instrument

Bruker Avance - 700 MHz 4 mm triple-resonance MAS probe

Sample

State solid, Temperature 263 K, pH 7.2

#	Name	Isotope labeling	Type	Concentration
1	NFGAIL	[U-100% 13C; U-100% 15N]		2.5 mg
2	H2O	natural abundance		0.0 ~ 0.0 %
3	HEPES	natural abundance		0.0 ~ 0.0 mM

4 2D 13C-13C DARR 5 ms mixing

Instrument

Bruker Avance - 700 MHz 4 mm triple-resonance MAS probe

Sample

State solid, Temperature 263 K, pH 7.2

#	Name	Isotope labeling	Type	Concentration
1	NFGAIL	[U-100% 13C; U-100% 15N]		2.5 mg
2	H2O	natural abundance		0.0 ~ 0.0 %
3	HEPES	natural abundance		0.0 ~ 0.0 mM

5 2D 13C-13C DARR 20 ms mixing

Instrument

Bruker Avance - 700 MHz 4 mm triple-resonance MAS probe

Sample

State solid, Temperature 263 K, pH 7.2

#	Name	Isotope labeling	Type	Concentration
1	NFGAIL	[U-100% 13C; U-100% 15N]		2.5 mg
2	H2O	natural abundance		0.0 ~ 0.0 %
3	HEPES	natural abundance		0.0 ~ 0.0 mM

6 2D 13C-13C DARR 40 ms mixing

Instrument

Bruker Avance - 700 MHz 4 mm triple-resonance MAS probe

Sample

State solid, Temperature 263 K, pH 7.2

#	Name	Isotope labeling	Type	Concentration
1	NFGAIL	[U-100% 13C; U-100% 15N]		2.5 mg
2	H2O	natural abundance		0.0 ~ 0.0 %
3	HEPES	natural abundance		0.0 ~ 0.0 mM

7 2D 13C-13C DARR 100 ms mixing

Instrument

Bruker Avance - 700 MHz 4 mm triple-resonance MAS probe

Sample

State solid, Temperature 263 K, pH 7.2

#	Name	Isotope labeling	Type	Concentration
1	NFGAIL	[U-100% 13C; U-100% 15N]		2.5 mg
2	H2O	natural abundance		0.0 ~ 0.0 %
3	HEPES	natural abundance		0.0 ~ 0.0 mM

8 2D 13C-13C DARR 200 ms mixing

Instrument

Bruker Avance - 700 MHz 4 mm triple-resonance MAS probe

Sample

State solid, Temperature 263 K, pH 7.2

#	Name	Isotope labeling	Type	Concentration
1	NFGAIL	[U-100% 13C; U-100% 15N]		2.5 mg
2	H2O	natural abundance		0.0 ~ 0.0 %
3	HEPES	natural abundance		0.0 ~ 0.0 mM

nullKeywords Amylin, IAPP, Protein fibril, SNNFGAILSS, Symmetry