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Found 1 Document (1.657 seconds)

BMRB: 25000

2MPT

WW3 domain of Nedd4L in complex with its HECT domain PY motif

Authors

Escobedo, A., Macias, M.J., Gomes, T., Aragon, E., Martin-Malpartida, P., Ruiz, L.

Assembly

WW3 domain of Nedd4L in complex with its HECT domain PY motif

Entity

1. WW3 (polymer, Thiol state: not present), 48 monomers, 5656.434 Da Detail

GAMEQSFLPP GWEMRIAPNG RPFFIDHNTK TTTWEDPRLK FPVHMRSK

2. HECT PY (polymer, Thiol state: not present), 16 monomers, 2021.229 Da Detail

RLDLPPYETF EDLREK

Total weight

7677.663 Da

Max. entity weight

5656.434 Da

Source organism

Homo sapiens

Exptl. method

solution NMR

Refine. method

DGSA-distance geometry simulated annealing

Data set

assigned_chemical_shifts

Chem. Shift Complete1

Sequence coverage: 48.4 %, Completeness: 22.9 %, Completeness (bb): 17.7 % Detail

Polymer type: polypeptide(L)

	Total	¹H	¹³C	¹⁵N
All	22.9 % (183 of 799)	40.2 % (171 of 425)	3.2 % (10 of 313)	3.3 % (2 of 61)
Backbone	17.7 % (65 of 368)	47.2 % (58 of 123)	2.6 % (5 of 189)	3.6 % (2 of 56)
Sidechain	24.4 % (120 of 492)	37.4 % (113 of 302)	3.8 % (7 of 185)	0.0 % (0 of 5)
Aromatic	22.2 % (20 of 90)	44.4 % (20 of 45)	0.0 % (0 of 43)	0.0 % (0 of 2)
Methyl	28.3 % (13 of 46)	47.8 % (11 of 23)	8.7 % (2 of 23)

1. WW3

GAMEQSFLPP GWEMRIAPNG RPFFIDHNTK TTTWEDPRLK FPVHMRSK

2. HECT PY

RLDLPPYETF EDLREK

Show sample condition

Sample #1

Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 298 K, pH 7

#	Name	Isotope labeling	Concentration
1	WW3	natural abundance	0.7 mM
2	HECT_PY	natural abundance	1.4 mM
3	sodium chloride	natural abundance	150 mM
4	sodium phosphate	natural abundance	20 mM
5	H2O	natural abundance	90 %
6	D2O	natural abundance	10 %

Sample #2

Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 298 K, pH 7

#	Name	Isotope labeling	Concentration
7	WW3	[U-100% 15N]	1 mM
8	HECT_PY	natural abundance	2 mM
9	sodium chloride	natural abundance	150 mM
10	sodium phosphate	natural abundance	20 mM
11	H2O	natural abundance	90 %
12	D2O	natural abundance	10 %

Sample #3

Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 298 K, pH 7

#	Name	Isotope labeling	Concentration
13	WW3	[U-100% 13C; U-100% 15N]	0.8 mM
14	HECT_PY	natural abundance	1.6 mM
15	sodium chloride	natural abundance	150 mM
16	sodium phosphate	natural abundance	20 mM
17	H2O	natural abundance	90 %
18	D2O	natural abundance	10 %

Chem. Shift Complete2

Sequence coverage: 21.9 %, Completeness: 16.1 %, Completeness (bb): 12.9 % Detail

Polymer type: polypeptide(L)

	Total	¹H	¹³C	¹⁵N
All	16.1 % (257 of 1598)	27.1 % (230 of 850)	3.7 % (23 of 626)	3.3 % (4 of 122)
Backbone	12.9 % (95 of 736)	32.5 % (80 of 246)	2.9 % (11 of 378)	3.6 % (4 of 112)
Sidechain	17.2 % (169 of 984)	25.2 % (152 of 604)	4.6 % (17 of 370)	0.0 % (0 of 10)
Aromatic	13.9 % (25 of 180)	27.8 % (25 of 90)	0.0 % (0 of 86)	0.0 % (0 of 4)
Methyl	19.6 % (18 of 92)	34.8 % (16 of 46)	4.3 % (2 of 46)

1. WW3

GAMEQSFLPP GWEMRIAPNG RPFFIDHNTK TTTWEDPRLK FPVHMRSK

2. HECT PY

RLDLPPYETF EDLREK

Show sample condition

Sample #1

Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 298 K, pH 7

#	Name	Isotope labeling	Concentration
1	WW3	natural abundance	0.7 mM
2	HECT_PY	natural abundance	1.4 mM
3	sodium chloride	natural abundance	150 mM
4	sodium phosphate	natural abundance	20 mM
5	H2O	natural abundance	90 %
6	D2O	natural abundance	10 %

Sample #2

Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 298 K, pH 7

#	Name	Isotope labeling	Concentration
7	WW3	[U-100% 15N]	1 mM
8	HECT_PY	natural abundance	2 mM
9	sodium chloride	natural abundance	150 mM
10	sodium phosphate	natural abundance	20 mM
11	H2O	natural abundance	90 %
12	D2O	natural abundance	10 %

Sample #3

Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 298 K, pH 7

#	Name	Isotope labeling	Concentration
13	WW3	[U-100% 13C; U-100% 15N]	0.8 mM
14	HECT_PY	natural abundance	1.6 mM
15	sodium chloride	natural abundance	150 mM
16	sodium phosphate	natural abundance	20 mM
17	H2O	natural abundance	90 %
18	D2O	natural abundance	10 %

Chem. Shift Complete3

Sequence coverage: 57.8 %, Completeness: 17.0 %, Completeness (bb): 21.8 % Detail

Polymer type: polypeptide(L)

	Total	¹H	¹³C	¹⁵N
All	17.0 % (407 of 2397)	21.2 % (270 of 1275)	10.8 % (101 of 939)	19.7 % (36 of 183)
Backbone	21.8 % (241 of 1104)	31.4 % (116 of 369)	15.7 % (89 of 567)	21.4 % (36 of 168)
Sidechain	14.0 % (206 of 1476)	17.2 % (156 of 906)	9.0 % (50 of 555)	0.0 % (0 of 15)
Aromatic	9.3 % (25 of 270)	18.5 % (25 of 135)	0.0 % (0 of 129)	0.0 % (0 of 6)
Methyl	15.2 % (21 of 138)	24.6 % (17 of 69)	5.8 % (4 of 69)

1. WW3

GAMEQSFLPP GWEMRIAPNG RPFFIDHNTK TTTWEDPRLK FPVHMRSK

2. HECT PY

RLDLPPYETF EDLREK

Show sample condition

Sample #1

Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 298 K, pH 7

#	Name	Isotope labeling	Concentration
1	WW3	natural abundance	0.7 mM
2	HECT_PY	natural abundance	1.4 mM
3	sodium chloride	natural abundance	150 mM
4	sodium phosphate	natural abundance	20 mM
5	H2O	natural abundance	90 %
6	D2O	natural abundance	10 %

Sample #2

Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 298 K, pH 7

#	Name	Isotope labeling	Concentration
7	WW3	[U-100% 15N]	1 mM
8	HECT_PY	natural abundance	2 mM
9	sodium chloride	natural abundance	150 mM
10	sodium phosphate	natural abundance	20 mM
11	H2O	natural abundance	90 %
12	D2O	natural abundance	10 %

Sample #3

Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 298 K, pH 7

#	Name	Isotope labeling	Concentration
13	WW3	[U-100% 13C; U-100% 15N]	0.8 mM
14	HECT_PY	natural abundance	1.6 mM
15	sodium chloride	natural abundance	150 mM
16	sodium phosphate	natural abundance	20 mM
17	H2O	natural abundance	90 %
18	D2O	natural abundance	10 %

Protein Blocks Logo

Calculated from 20 models in PDB: 2MPT, Strand ID: A, B Detail

Release date

2014-10-19

Citation

Structural basis of the activation and degradation mechanisms of the E3 ubiquitin ligase Nedd4L
Escobedo, A., Gomes, T., Aragon, E., Martin-Malpartida, P., Ruiz, L., Macias, M.
Structure (2014), 22, 1446-1457, PubMed 25295397 , DOI 10.1016/j.str.2014.08.016 , Abstract

Related entities 1. WW3, : 6 : 47 entities Detail

Related entities 2. HECT PY, : 1 : 10 : 1 : 77 entities Detail

Interaction partners 1. WW3, : 10 interactors Detail

More details for 10 interactors identified by 4 citations

Interaction partners 2. HECT PY, : 10 interactors Detail

More details for 10 interactors identified by 4 citations

Experiments performed 6 experiments Detail

1 2D 1H-1H NOESY

Instrument

Bruker Avance - 600 MHz

Sample

State isotropic, Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 298 K, pH 7

#	Name	Isotope labeling	Concentration
1	WW3	natural abundance	0.7 mM
2	HECT_PY	natural abundance	1.4 mM
3	sodium chloride	natural abundance	150 mM
4	sodium phosphate	natural abundance	20 mM
5	H2O	natural abundance	90 %
6	D2O	natural abundance	10 %

2 2D 1H-1H TOCSY

Instrument

Bruker Avance - 600 MHz

Sample

State isotropic, Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 298 K, pH 7

#	Name	Isotope labeling	Concentration
1	WW3	natural abundance	0.7 mM
2	HECT_PY	natural abundance	1.4 mM
3	sodium chloride	natural abundance	150 mM
4	sodium phosphate	natural abundance	20 mM
5	H2O	natural abundance	90 %
6	D2O	natural abundance	10 %

3 3D 1H-15N NOESY

Instrument

Bruker Avance - 600 MHz

Sample

State isotropic, Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 298 K, pH 7

#	Name	Isotope labeling	Concentration
7	WW3	[U-100% 15N]	1 mM
8	HECT_PY	natural abundance	2 mM
9	sodium chloride	natural abundance	150 mM
10	sodium phosphate	natural abundance	20 mM
11	H2O	natural abundance	90 %
12	D2O	natural abundance	10 %

4 3D 1H-15N TOCSY

Instrument

Bruker Avance - 600 MHz

Sample

State isotropic, Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 298 K, pH 7

#	Name	Isotope labeling	Concentration
7	WW3	[U-100% 15N]	1 mM
8	HECT_PY	natural abundance	2 mM
9	sodium chloride	natural abundance	150 mM
10	sodium phosphate	natural abundance	20 mM
11	H2O	natural abundance	90 %
12	D2O	natural abundance	10 %

5 3D HNCACB

Instrument

Bruker Avance - 600 MHz

Sample

State isotropic, Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 298 K, pH 7

#	Name	Isotope labeling	Concentration
13	WW3	[U-100% 13C; U-100% 15N]	0.8 mM
14	HECT_PY	natural abundance	1.6 mM
15	sodium chloride	natural abundance	150 mM
16	sodium phosphate	natural abundance	20 mM
17	H2O	natural abundance	90 %
18	D2O	natural abundance	10 %

6 3D CBCA(CO)NH

Instrument

Bruker Avance - 600 MHz

Sample

State isotropic, Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 298 K, pH 7

#	Name	Isotope labeling	Concentration
13	WW3	[U-100% 13C; U-100% 15N]	0.8 mM
14	HECT_PY	natural abundance	1.6 mM
15	sodium chloride	natural abundance	150 mM
16	sodium phosphate	natural abundance	20 mM
17	H2O	natural abundance	90 %
18	D2O	natural abundance	10 %

nullKeywords WW, Nedd4L, Nedd4.2, HECT, PY, WW3, Auto-ubiquitination, Proteasomal degradation, Ubiquitination, Ubiquitin ligase

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Found 1 Document (1.657 seconds)

BMRB: 25000

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Related entities 1. WW3, : 6 : 47 entities Detail

Related entities 2. HECT PY, : 1 : 10 : 1 : 77 entities Detail

Interaction partners 1. WW3, : 10 interactors Detail

Interaction partners 2. HECT PY, : 10 interactors Detail

Experiments performed 6 experiments Detail

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