BMRB: 25004

Chemical shift assignments for the parallel in-register D23N Iowa Mutant Beta Amyloid Fibrils

Authors

Sgourakis, N., Qiang, W.

Assembly

D23N parallel

Entity

1. D23N parallel (polymer, Thiol state: not present), 26 monomers, 2648.126 Da Detail

QKLVFFAENV GSNKGAIIGL MVGGVV

Formula weight

2648.126 Da

Source organism

Homo sapiens

Exptl. method

NMR

Refine. method

Rosetta refinement

Data set

assigned_chemical_shifts

Chem. Shift Complete

Sequence coverage: 80.8 %, Completeness: 51.3 %, Completeness (bb): 78.1 % Detail

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Polymer type: polypeptide(L)

	Total	13C
All	51.3 % (59 of 115)	51.3 % (59 of 115)
Backbone	78.1 % (57 of 73)	78.1 % (57 of 73)
Sidechain	30.2 % (19 of 63)	30.2 % (19 of 63)
Aromatic	0.0 % (0 of 10)	0.0 % (0 of 10)
Methyl	20.0 % (4 of 20)	20.0 % (4 of 20)

1. D23N Abeta

QKLVFFAENV GSNKGAIIGL MVGGVV

Show sample condition

Sample

Solvent system solid, Temperature 298 K, pH 7.4

#	Name	Isotope labeling	Type	Concentration
1	D23N_Abeta	[U-100% 13C; U-100% 15N]		25.0 ~ 100.0 uM

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Protein Blocks Logo

Calculated from 5 models in PDB: 2MPZ, Strand ID: A, B, C, D, E, F, G, H, I, J, K, L, M, N, O, P, Q, R, S, T, U, V, W, X, Y, Z Detail

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Release date

2015-04-19

Citation

Modeling an in-register, parallel "iowa" aβ fibril structure using solid-state NMR data from labeled samples with rosetta
Sgourakis, N., Yau, W., Qiang, W.
Structure (2015), 23, 216-227, PubMed 25543257 , DOI 10.1016/j.str.2014.10.022 , Abstract

Related entities 1. D23N parallel, : 1 : 1 : 88 : 16 entities Detail

Interaction partners 1. D23N parallel, : 2 interactors Detail

Experiments performed 3 experiments Detail