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BMRB: 25007

2MQ0

STRUCTURAL CHARACTERIZATION OF THE C3 DOMAIN OF CARDIAC MYOSIN BINDING PROTEIN-C

Authors

Zhang, X., De, S., McIntosh, L., Paetzel, M.

Assembly

C3 DOMAIN OF CARDIAC MYOSIN BINDING PROTEIN-C, WT

Entity

1. C3 DOMAIN OF CARDIAC MYOSIN BINDING PROTEIN-C, WT (polymer, Thiol state: all free), 95 monomers, 10812.20 Da Detail

GSHMPVLITR PLEDQLVMVG QRVEFECEVS EEGAQVKWLK DGVELTREET FKYRFKKDGQ RHHLIINEAM LEDAGHYALC TSGGQALAEL IVQEK

Formula weight

10812.2 Da

Source organism

Homo sapiens

Exptl. method

solution NMR

Refine. method

torsion angle dynamics

Data set

assigned_chemical_shifts

Chem. Shift Complete

Sequence coverage: 97.9 %, Completeness: 96.8 %, Completeness (bb): 97.0 % Detail

Polymer type: polypeptide(L)

	Total	¹H	¹³C	¹⁵N
All	96.8 % (1078 of 1114)	96.2 % (559 of 581)	97.7 % (422 of 432)	96.0 % (97 of 101)
Backbone	97.0 % (549 of 566)	95.9 % (188 of 196)	98.2 % (272 of 277)	95.7 % (89 of 93)
Sidechain	96.9 % (615 of 635)	96.4 % (371 of 385)	97.5 % (236 of 242)	100.0 % (8 of 8)
Aromatic	97.3 % (72 of 74)	100.0 % (37 of 37)	94.4 % (34 of 36)	100.0 % (1 of 1)
Methyl	100.0 % (108 of 108)	100.0 % (54 of 54)	100.0 % (54 of 54)

1. entity

GSHMPVLITR PLEDQLVMVG QRVEFECEVS EEGAQVKWLK DGVELTREET FKYRFKKDGQ RHHLIINEAM LEDAGHYALC TSGGQALAEL IVQEK

Show sample condition

Sample

Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 298 K, pH 6.5

#	Name	Isotope labeling	Concentration
1	sodium phosphate	natural abundance	50 mM
2	sodium chloride	natural abundance	100 mM
3	protein	[U-100% 13C; U-100% 15N]	0.8 mM
4	H2O	natural abundance	90 %
5	D2O	natural abundance	10 %

LACS Plot; CA

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹H	DSS	methyl protons	external	direct	1.0
¹³C	DSS	methyl protons	external	indirect	0.2514495
¹⁵N	DSS	methyl protons	external	indirect	0.1013292

Referencing offset: -0.28 ppm, Outliers: 1 Detail

LACS Plot; CB

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹H	DSS	methyl protons	external	direct	1.0
¹³C	DSS	methyl protons	external	indirect	0.2514495
¹⁵N	DSS	methyl protons	external	indirect	0.1013292

Referencing offset: -0.28 ppm, Outliers: 1 Detail

LACS Plot; HA

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹H	DSS	methyl protons	external	direct	1.0
¹³C	DSS	methyl protons	external	indirect	0.2514495
¹⁵N	DSS	methyl protons	external	indirect	0.1013292

Referencing offset: -0.07 ppm, Outliers: 1 Detail

LACS Plot; CO

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹H	DSS	methyl protons	external	direct	1.0
¹³C	DSS	methyl protons	external	indirect	0.2514495
¹⁵N	DSS	methyl protons	external	indirect	0.1013292

Referencing offset: 0.53 ppm, Outliers: 1 Detail

Protein Blocks Logo

Calculated from 10 models in PDB: 2MQ0, Strand ID: A Detail

Release date

2014-07-27

Citation

Structural characterization of the C3 domain of cardiac myosin binding protein C and its hypertrophic cardiomyopathy-related R502W mutant
Zhang, X., De, S., McIntosh, L., Paetzel, M.
Biochemistry (2014), 53, 5332-5342, PubMed 25058872 , DOI 10.1021/bi500784g , Abstract

Entries sharing articles BMRB: 1 entries Detail

BMRB: 25010 released on 2014-07-27
Title Structural Characterization of the Hypertrophic Cardiomyopathy-Related R502W Mutant of the C3 Domain of Cardiac Myosin Binding Protein-C

Related entities 1. C3 DOMAIN OF CARDIAC MYOSIN BINDING PROTEIN-C, WT, : 1 : 3 : 397 entities Detail

Interaction partners 1. C3 DOMAIN OF CARDIAC MYOSIN BINDING PROTEIN-C, WT, : 5 interactors Detail

More details for 5 interactors identified by 4 citations

Experiments performed 9 experiments Detail

1 2D 1H-15N HSQC