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BMRB: 25027

2MQC

NMR structure of the hypothetical protein BVU_0925 from Bacteroides vulgatus ATCC 8482

Authors

Dutta, S.K., Serrano, P., Geralt, M., Wuthrich, K.

Assembly

NMR structure of the hypothetical protein BVU 0925 from Bacteroides vulgatus ATCC 8482

Entity

1. NMR structure of the hypothetical protein BVU 0925 from Bacteroides vulgatus ATCC 8482 (polymer, Thiol state: all free), 105 monomers, 11283.14 Da Detail

GHMGSPVSYY FSYADGGTSH TEYPDDSSAG SFILDITSYK KTGNSTKALS WNASGDSWIH VNGSSVSYDE NPAKERRTGL VTLKQDESGK TLSLKIVQPG KTSID

Formula weight

11283.14 Da

Source organism

Phocaeicola vulgatus ATCC 8482

Exptl. method

solution NMR

Refine. method

molecular dynamics

Data set

assigned_chemical_shifts

Chem. Shift Complete

Sequence coverage: 100.0 %, Completeness: 88.2 %, Completeness (bb): 84.6 % Detail

Polymer type: polypeptide(L)

	Total	¹H	¹³C	¹⁵N
All	88.2 % (1014 of 1150)	95.1 % (562 of 591)	76.7 % (345 of 450)	98.2 % (107 of 109)
Backbone	84.6 % (526 of 622)	97.7 % (212 of 217)	70.7 % (215 of 304)	98.0 % (99 of 101)
Sidechain	93.4 % (581 of 622)	93.6 % (350 of 374)	92.9 % (223 of 240)	100.0 % (8 of 8)
Aromatic	78.8 % (82 of 104)	78.8 % (41 of 52)	78.0 % (39 of 50)	100.0 % (2 of 2)
Methyl	100.0 % (92 of 92)	100.0 % (46 of 46)	100.0 % (46 of 46)

1. entity

GHMGSPVSYY FSYADGGTSH TEYPDDSSAG SFILDITSYK KTGNSTKALS WNASGDSWIH VNGSSVSYDE NPAKERRTGL VTLKQDESGK TLSLKIVQPG KTSID

Show sample condition

Sample

Solvent system 95% H2O/5% D2O, Pressure 1 atm, Temperature 308 K, pH 6.0

#	Name	Isotope labeling	Concentration
1	entity	[U-99% 13C; U-98% 15N]	1.2 mM
2	sodium phosphate	natural abundance	20 mM
3	sodium chloride	natural abundance	50 mM
4	sodium azide	natural abundance	5 mM
5	H2O	natural abundance	95 %
6	D2O	natural abundance	5 %

LACS Plot; CA

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹H	DSS	methyl protons	internal	direct	1.0
¹³C	DSS	methyl protons	na	indirect	0.2514495
¹⁵N	DSS	methyl protons	na	indirect	0.1013291

Referencing offset: 2.65 ppm, Outliers: 1 Detail

LACS Plot; CB

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹H	DSS	methyl protons	internal	direct	1.0
¹³C	DSS	methyl protons	na	indirect	0.2514495
¹⁵N	DSS	methyl protons	na	indirect	0.1013291

Referencing offset: 2.65 ppm, Outliers: 1 Detail

LACS Plot; HA

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹H	DSS	methyl protons	internal	direct	1.0
¹³C	DSS	methyl protons	na	indirect	0.2514495
¹⁵N	DSS	methyl protons	na	indirect	0.1013291

Referencing offset: 0.0 ppm, Outliers: 2 Detail

Protein Blocks Logo

Calculated from 20 models in PDB: 2MQC, Strand ID: A Detail

Release date

2014-09-01

Citation

NMR structure of the hypothetical protein BVU_0925 from Bacteroides vulgatus ATCC 8482
Dutta, S.K., Serrano, P., Geralt, M., Wuthrich, K.
Not known

Related entities 1. NMR structure of the hypothetical protein BVU 0925 from Bacteroides vulgatus ATCC 8482, : 1 entities Detail

Experiments performed 7 experiments Detail

1 APSY 4D-HACANH