Solution structure of the Chlamydomonas reinhardtii NAB1 cold shock domain, CSD1
Polymer type: polypeptide(L)
Total | 1H | 13C | 15N | |
---|---|---|---|---|
All | 77.2 % (707 of 916) | 77.7 % (369 of 475) | 75.6 % (266 of 352) | 80.9 % (72 of 89) |
Backbone | 81.4 % (397 of 488) | 81.8 % (144 of 176) | 80.6 % (187 of 232) | 82.5 % (66 of 80) |
Sidechain | 73.8 % (366 of 496) | 75.3 % (225 of 299) | 71.8 % (135 of 188) | 66.7 % (6 of 9) |
Aromatic | 34.0 % (34 of 100) | 40.0 % (20 of 50) | 26.5 % (13 of 49) | 100.0 % (1 of 1) |
Methyl | 100.0 % (64 of 64) | 100.0 % (32 of 32) | 100.0 % (32 of 32) |
1. NAB1 cold shock domain
MRGSHHHHHH GSGEQLRQQG TVKWFNATKG FGFITPGGGG EDLFVHQTNI NSEGFRSLRE GEVVEFEVEA GPDGRSKAVN VTSolvent system 95% H2O/5% D2O, Pressure 1 atm, Temperature 298 K, pH 7
# | Name | Isotope labeling | Type | Concentration |
---|---|---|---|---|
1 | NAB1 cold shock domain | [U-100% 13C; U-100% 15N] | 267 uM | |
2 | sodium phosphate | natural abundance | 20 mM | |
3 | sodium chloride | natural abundance | 300 mM | |
4 | H2O | natural abundance | 95 % | |
5 | D2O | natural abundance | 5 % |