BMRB: 25047

RRM-Peptide and RES Complex

Authors

Tripsianes, K., Friberg, A., Barrandon, C., Seraphin, B., Sattler, M.

Assembly

RRM-PEPTIDE and RES COMPLEX

Entity

1. RRM (polymer, Thiol state: not present), 93 monomers, 10526.60 Da Detail

GAMGNEYKDN AYIYIGNLNR ELTEGDILTV FSEYGVPVDV ILSRDENTGE SQGFAYLKYE DQRSTILAVD NLNGFKIGGR ALKIDHTFYR PKR

2. RES (polymer, Thiol state: not present), 39 monomers, 4524.021 Da Detail

GAMGNRFAIM PGSRWDGVHR SNGFEEKWFA KQNEINEKK

Total weight

15050.621 Da

Max. entity weight

10526.6 Da

Source organism

Saccharomyces cerevisiae

Exptl. method

solution NMR

Refine. method

SIMULATED ANNEALING

Data set

assigned_chemical_shifts

Chem. Shift Complete

Sequence coverage: 93.9 %, Completeness: 83.5 %, Completeness (bb): 78.0 % Detail

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Polymer type: polypeptide(L)

	Total	1H	13C	15N
All	83.5 % (1300 of 1556)	90.3 % (735 of 814)	72.9 % (435 of 597)	89.7 % (130 of 145)
Backbone	78.0 % (613 of 786)	90.9 % (251 of 276)	64.0 % (244 of 381)	91.5 % (118 of 129)
Sidechain	89.5 % (794 of 887)	89.6 % (482 of 538)	90.1 % (300 of 333)	75.0 % (12 of 16)
Aromatic	89.9 % (142 of 158)	91.1 % (72 of 79)	88.3 % (68 of 77)	100.0 % (2 of 2)
Methyl	91.5 % (108 of 118)	89.8 % (53 of 59)	93.2 % (55 of 59)

1. RRM

GAMGNEYKDN AYIYIGNLNR ELTEGDILTV FSEYGVPVDV ILSRDENTGE SQGFAYLKYE DQRSTILAVD NLNGFKIGGR ALKIDHTFYR PKR

2. RES

GAMGNRFAIM PGSRWDGVHR SNGFEEKWFA KQNEINEKK

Show sample condition

Sample

Solvent system 93% H2O/7% D2O, Pressure 1.000 atm, Temperature 298.000 K, pH 6.300, Details 0.5mM Snu17p-Bud13p stoichiometric complex

#	Name	Isotope labeling	Type	Concentration
1	RRM	[U-13C; U-15N]		0.5 mM
2	RES	[U-13C; U-15N]		0.5 mM
3	H2O	natural abundance		93 %
4	D2O	[U-100% 2H]		7 %

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LACS Plot; CA

Referencing offset: 0.41 ppm, Outliers: 1 Detail

Show chemical shift referencing parameters

Chemical shift reference

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
1H	DSS	methyl protons	0.0 ppm	internal	direct	1.0
13C	DSS	methyl protons	0.0 ppm	na	indirect	0.2514495
15N	DSS	methyl protons	0.0 ppm	na	indirect	0.1013291

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LACS Plot; CB

Referencing offset: 0.41 ppm, Outliers: 1 Detail

Show chemical shift referencing parameters

Chemical shift reference

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
1H	DSS	methyl protons	0.0 ppm	internal	direct	1.0
13C	DSS	methyl protons	0.0 ppm	na	indirect	0.2514495
15N	DSS	methyl protons	0.0 ppm	na	indirect	0.1013291

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LACS Plot; HA

Referencing offset: 0.12 ppm, Outliers: 3 Detail

Show chemical shift referencing parameters

Chemical shift reference

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
1H	DSS	methyl protons	0.0 ppm	internal	direct	1.0
13C	DSS	methyl protons	0.0 ppm	na	indirect	0.2514495
15N	DSS	methyl protons	0.0 ppm	na	indirect	0.1013291

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Protein Blocks Logo

Calculated from 20 models in PDB: 4UQT, Strand ID: A, B Detail

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Release date

2014-08-25

Citation

A Novel RRM-Peptide Interaction in the RES Complex
Tripsianes, K., Friberg, A., Barrandon, C., Seraphin, B., Sattler, M.
J. Biol. Chem.

Related entities 1. RRM, : 102 entities Detail

Related entities 2. RES, : 1 : 5 : 15 entities Detail

Interaction partners 2. RES, : 19 interactors Detail

Experiments performed 4 experiments Detail