BMRB: 25049

A Structure based mechanism for tRNA and retroviral RNA remodeling during primer annealing

Authors

D'Souza, V., Yildiz, Z.

Assembly

RNA (68-MER)

Entity

1. RNA (68-MER) (polymer, Thiol state: not present), 68 monomers, 21956.85 Da Detail

GGGCGAGGGU CUCCUCUGAG UGAUUGACUA CCCGUCAGCG GGGGUCUUUC AUUUGGGGGC UCGUGCCC

Formula weight

21956.85 Da

Source organism

Murine leukemia virus

Exptl. method

solution NMR

Data set

assigned_chemical_shifts

Chem. Shift Complete1

Sequence coverage: 95.6 %, Completeness: 31.4 %, Completeness (bb): 20.2 % Detail

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Polymer type: polyribonucleotide

	Total	1H	13C	15N
All	31.4 % (343 of 1092)	31.9 % (191 of 598)	33.5 % (151 of 451)	2.3 % (1 of 43)
Suger, PO4	20.2 % (151 of 748)	22.1 % (90 of 408)	17.9 % (61 of 340)
Nucleobase	55.8 % (192 of 344)	53.2 % (101 of 190)	81.1 % (90 of 111)	2.3 % (1 of 43)
Aromatic	62.3 % (192 of 308)	65.6 % (101 of 154)	81.1 % (90 of 111)	2.3 % (1 of 43)

1. RNA (68-MER)

GGGCGAGGGU CUCCUCUGAG UGAUUGACUA CCCGUCAGCG GGGGUCUUUC AUUUGGGGGC UCGUGCCC

Show sample condition

Sample

Solvent system 100% D2O, Pressure 1 atm, Temperature 311 K, pH 7.2

#	Name	Isotope labeling	Type	Concentration
1	U5-PBS	[U-100% 13C; U-100% 15N]		0.8 mM
2	Tris	natural abundance		10 mM
3	NaCl	natural abundance		10 mM
4	D2O	natural abundance		100 %

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Chem. Shift Complete2

Sequence coverage: 95.6 %, Completeness: 32.0 %, Completeness (bb): 20.7 % Detail

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Polymer type: polyribonucleotide

	Total	1H	13C	15N
All	32.0 % (698 of 2184)	32.6 % (390 of 1196)	33.8 % (305 of 902)	3.5 % (3 of 86)
Suger, PO4	20.7 % (309 of 1496)	22.7 % (185 of 816)	18.2 % (124 of 680)
Nucleobase	56.5 % (389 of 688)	53.9 % (205 of 380)	81.5 % (181 of 222)	3.5 % (3 of 86)
Aromatic	62.8 % (387 of 616)	65.9 % (203 of 308)	81.5 % (181 of 222)	3.5 % (3 of 86)

1. RNA (68-MER)

GGGCGAGGGU CUCCUCUGAG UGAUUGACUA CCCGUCAGCG GGGGUCUUUC AUUUGGGGGC UCGUGCCC

Show sample condition

Sample

Solvent system 100% D2O, Pressure 1 atm, Temperature 311 K, pH 7.2

#	Name	Isotope labeling	Type	Concentration
1	U5-PBS	[U-100% 13C; U-100% 15N]		0.8 mM
2	Tris	natural abundance		10 mM
3	NaCl	natural abundance		10 mM
4	D2O	natural abundance		100 %

Hide sample codition

Chem. Shift Complete3

Sequence coverage: 39.7 %, Completeness: 23.5 %, Completeness (bb): 14.3 % Detail

Download

• NMR-STAR v3.1 (partial)
• BMRB/XML (partial)
• CSV
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Polymer type: polyribonucleotide

	Total	1H	13C	15N
All	23.5 % (769 of 3276)	23.6 % (424 of 1794)	23.3 % (315 of 1353)	23.3 % (30 of 129)
Suger, PO4	14.3 % (321 of 2244)	15.5 % (190 of 1224)	12.8 % (131 of 1020)
Nucleobase	43.4 % (448 of 1032)	41.1 % (234 of 570)	55.3 % (184 of 333)	23.3 % (30 of 129)
Aromatic	48.3 % (446 of 924)	50.2 % (232 of 462)	55.3 % (184 of 333)	23.3 % (30 of 129)

1. RNA (68-MER)

GGGCGAGGGU CUCCUCUGAG UGAUUGACUA CCCGUCAGCG GGGGUCUUUC AUUUGGGGGC UCGUGCCC

Show sample condition

Sample

Solvent system 100% D2O, Pressure 1 atm, Temperature 311 K, pH 7.2

#	Name	Isotope labeling	Type	Concentration
1	U5-PBS	[U-100% 13C; U-100% 15N]		0.8 mM
2	Tris	natural abundance		10 mM
3	NaCl	natural abundance		10 mM
4	D2O	natural abundance		100 %

Hide sample codition

Release date

2015-10-15

Citation

A structure-based mechanism for tRNA and retroviral RNA remodelling during primer annealing
Miller, S.B., Yildiz, F.ZEHRA., Lo, J.A., Wang, B., D'Souza, V.M.
Nature (2014), 515, 591-595, PubMed 25209668 , DOI 10.1038/nature13709 , Abstract

Related entities 1. RNA (68-MER), : 1 : 1 entities Detail

Experiments performed 5 experiments Detail