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BMRB: 25064

2MR7

apo structure of the Peptidyl Carrier Protein Domain 7 of the teicoplanin producing Non-ribosomal peptide synthetase

Authors

Haslinger, K., Maximowitsch, E., Redfield, C., Cryle, M.J.

Assembly

PCP7T

Entity

1. PCP7T (polymer, Thiol state: all free), 91 monomers, 9618.939 Da Detail

GAMAKAPESA TEKVLCALYA EILGVERVGV DDAFHDLGGS SALAMRLIAR IREELGVDLP IRQLFSSPTP AGVARALAAK SASWSHPQFE K

Formula weight

9618.939 Da

Source organism

Actinoplanes teichomyceticus

Exptl. method

solution NMR

Refine. method

molecular dynamics

Data set

assigned_chemical_shifts

Chem. Shift Complete1

Sequence coverage: 82.4 %, Completeness: 44.3 %, Completeness (bb): 33.4 % Detail

Polymer type: polypeptide(L)

	Total	¹H	¹³C	¹⁵N
All	44.3 % (443 of 1001)	64.9 % (334 of 515)	21.9 % (87 of 397)	24.7 % (22 of 89)
Backbone	33.4 % (179 of 536)	51.6 % (95 of 184)	23.3 % (62 of 266)	25.6 % (22 of 86)
Sidechain	51.9 % (285 of 549)	72.2 % (239 of 331)	21.4 % (46 of 215)	0.0 % (0 of 3)
Aromatic	53.4 % (31 of 58)	89.7 % (26 of 29)	17.9 % (5 of 28)	0.0 % (0 of 1)
Methyl	54.3 % (63 of 116)	89.7 % (52 of 58)	19.0 % (11 of 58)

1. PCP7T

GAMAKAPESA TEKVLCALYA EILGVERVGV DDAFHDLGGS SALAMRLIAR IREELGVDLP IRQLFSSPTP AGVARALAAK SASWSHPQFE K

Show sample condition

Sample #1

Solvent system 95% H2O/5% D2O, Pressure 1 atm, Temperature 293 K, pH 7.4

#	Name	Isotope labeling	Concentration
1	PCP7T	[U-100% 13C; U-100% 15N]	350.0 ~ 500.0 uM
2	TRIS	natural abundance	50 mM
3	H2O	natural abundance	95 %
4	D2O	natural abundance	5 %

Sample #2

Solvent system 95% H2O/5% D2O, Pressure 1 atm, Temperature 293 K, pH 7.4

#	Name	Isotope labeling	Concentration
5	PCP7T	natural abundance	500 uM
6	TRIS	natural abundance	50 mM
7	H2O	natural abundance	95 %
8	D2O	natural abundance	5 %

Sample #3

Solvent system 100% D2O, Pressure 1 atm, Temperature 293 K, pH 7.4

#	Name	Isotope labeling	Concentration
9	PCP7T	natural abundance	500 uM
10	TRIS	natural abundance	50 mM
11	D2O	natural abundance	100 %

Chem. Shift Complete2

Sequence coverage: 91.2 %, Completeness: 54.5 %, Completeness (bb): 47.0 % Detail

Polymer type: polypeptide(L)

	Total	¹H	¹³C	¹⁵N
All	54.5 % (1092 of 2002)	69.9 % (720 of 1030)	40.1 % (318 of 794)	30.3 % (54 of 178)
Backbone	47.0 % (504 of 1072)	63.6 % (234 of 368)	40.6 % (216 of 532)	31.4 % (54 of 172)
Sidechain	61.6 % (676 of 1098)	73.4 % (486 of 662)	44.2 % (190 of 430)	0.0 % (0 of 6)
Aromatic	44.0 % (51 of 116)	70.7 % (41 of 58)	17.9 % (10 of 56)	0.0 % (0 of 2)
Methyl	66.4 % (154 of 232)	90.5 % (105 of 116)	42.2 % (49 of 116)

1. PCP7T

GAMAKAPESA TEKVLCALYA EILGVERVGV DDAFHDLGGS SALAMRLIAR IREELGVDLP IRQLFSSPTP AGVARALAAK SASWSHPQFE K

Show sample condition

Sample #1

Solvent system 95% H2O/5% D2O, Pressure 1 atm, Temperature 293 K, pH 7.4

#	Name	Isotope labeling	Concentration
1	PCP7T	[U-100% 13C; U-100% 15N]	350.0 ~ 500.0 uM
2	TRIS	natural abundance	50 mM
3	H2O	natural abundance	95 %
4	D2O	natural abundance	5 %

Sample #2

Solvent system 95% H2O/5% D2O, Pressure 1 atm, Temperature 293 K, pH 7.4

#	Name	Isotope labeling	Concentration
5	PCP7T	natural abundance	500 uM
6	TRIS	natural abundance	50 mM
7	H2O	natural abundance	95 %
8	D2O	natural abundance	5 %

Sample #3

Solvent system 100% D2O, Pressure 1 atm, Temperature 293 K, pH 7.4

#	Name	Isotope labeling	Concentration
9	PCP7T	natural abundance	500 uM
10	TRIS	natural abundance	50 mM
11	D2O	natural abundance	100 %

Chem. Shift Complete3

Sequence coverage: 85.7 %, Completeness: 53.8 %, Completeness (bb): 47.6 % Detail

Polymer type: polypeptide(L)

	Total	¹H	¹³C	¹⁵N
All	53.8 % (1615 of 3003)	70.1 % (1083 of 1545)	37.4 % (445 of 1191)	32.6 % (87 of 267)
Backbone	47.6 % (765 of 1608)	67.8 % (374 of 552)	38.1 % (304 of 798)	33.7 % (87 of 258)
Sidechain	58.8 % (968 of 1647)	71.4 % (709 of 993)	40.2 % (259 of 645)	0.0 % (0 of 9)
Aromatic	42.0 % (73 of 174)	72.4 % (63 of 87)	11.9 % (10 of 84)	0.0 % (0 of 3)
Methyl	65.8 % (229 of 348)	90.8 % (158 of 174)	40.8 % (71 of 174)

1. PCP7T

GAMAKAPESA TEKVLCALYA EILGVERVGV DDAFHDLGGS SALAMRLIAR IREELGVDLP IRQLFSSPTP AGVARALAAK SASWSHPQFE K

Show sample condition

Sample #1

Solvent system 95% H2O/5% D2O, Pressure 1 atm, Temperature 293 K, pH 7.4

#	Name	Isotope labeling	Concentration
1	PCP7T	[U-100% 13C; U-100% 15N]	350.0 ~ 500.0 uM
2	TRIS	natural abundance	50 mM
3	H2O	natural abundance	95 %
4	D2O	natural abundance	5 %

Sample #2

Solvent system 95% H2O/5% D2O, Pressure 1 atm, Temperature 293 K, pH 7.4

#	Name	Isotope labeling	Concentration
5	PCP7T	natural abundance	500 uM
6	TRIS	natural abundance	50 mM
7	H2O	natural abundance	95 %
8	D2O	natural abundance	5 %

Sample #3

Solvent system 100% D2O, Pressure 1 atm, Temperature 293 K, pH 7.4

#	Name	Isotope labeling	Concentration
9	PCP7T	natural abundance	500 uM
10	TRIS	natural abundance	50 mM
11	D2O	natural abundance	100 %

Chem. Shift Complete4

Sequence coverage: 95.6 %, Completeness: 54.1 %, Completeness (bb): 50.0 % Detail

Polymer type: polypeptide(L)

	Total	¹H	¹³C	¹⁵N
All	54.1 % (2168 of 4004)	71.7 % (1478 of 2060)	33.0 % (524 of 1588)	46.6 % (166 of 356)
Backbone	50.0 % (1072 of 2144)	73.0 % (537 of 736)	34.8 % (370 of 1064)	48.0 % (165 of 344)
Sidechain	56.2 % (1234 of 2196)	71.1 % (941 of 1324)	34.0 % (292 of 860)	8.3 % (1 of 12)
Aromatic	35.8 % (83 of 232)	62.9 % (73 of 116)	8.9 % (10 of 112)	0.0 % (0 of 4)
Methyl	62.7 % (291 of 464)	90.5 % (210 of 232)	34.9 % (81 of 232)

1. PCP7T

GAMAKAPESA TEKVLCALYA EILGVERVGV DDAFHDLGGS SALAMRLIAR IREELGVDLP IRQLFSSPTP AGVARALAAK SASWSHPQFE K

Show sample condition

Sample #1

Solvent system 95% H2O/5% D2O, Pressure 1 atm, Temperature 293 K, pH 7.4

#	Name	Isotope labeling	Concentration
1	PCP7T	[U-100% 13C; U-100% 15N]	350.0 ~ 500.0 uM
2	TRIS	natural abundance	50 mM
3	H2O	natural abundance	95 %
4	D2O	natural abundance	5 %

Sample #2

Solvent system 95% H2O/5% D2O, Pressure 1 atm, Temperature 293 K, pH 7.4

#	Name	Isotope labeling	Concentration
5	PCP7T	natural abundance	500 uM
6	TRIS	natural abundance	50 mM
7	H2O	natural abundance	95 %
8	D2O	natural abundance	5 %

Sample #3

Solvent system 100% D2O, Pressure 1 atm, Temperature 293 K, pH 7.4

#	Name	Isotope labeling	Concentration
9	PCP7T	natural abundance	500 uM
10	TRIS	natural abundance	50 mM
11	D2O	natural abundance	100 %

Protein Blocks Logo

Calculated from 20 models in PDB: 2MR7, Strand ID: A Detail

Release date

2015-01-25

Citation

Structure of the terminal PCP domain of the non-ribosomal peptide synthetase in teicoplanin biosynthesis
Haslinger, K., Maximowitsch, E., Brieke, C., Koch, A., Redfield, C., Cryle, M.J.
Proteins (2015), 83, 711-721, PubMed 25586301 , DOI 10.1002/prot.24758 , Abstract

Related entities 1. PCP7T, : 1 : 1 : 123 entities Detail

Experiments performed 17 experiments Detail

1 3D 1H-15N NOESY

Instrument

Bruker Bruker - 600 MHz

Sample

State isotropic, Solvent system 95% H2O/5% D2O, Pressure 1 atm, Temperature 293 K, pH 7.4

#	Name	Isotope labeling	Concentration
1	PCP7T	[U-100% 13C; U-100% 15N]	350.0 ~ 500.0 uM
2	TRIS	natural abundance	50 mM
3	H2O	natural abundance	95 %
4	D2O	natural abundance	5 %

2 2D 1H-1H NOESY

Instrument

Bruker Bruker - 600 MHz

Sample

State isotropic, Solvent system 95% H2O/5% D2O, Pressure 1 atm, Temperature 293 K, pH 7.4

#	Name	Isotope labeling	Concentration
5	PCP7T	natural abundance	500 uM
6	TRIS	natural abundance	50 mM
7	H2O	natural abundance	95 %
8	D2O	natural abundance	5 %

3 2D 1H-1H NOESY

Instrument

Bruker Bruker - 600 MHz

Sample

State isotropic, Solvent system 100% D2O, Pressure 1 atm, Temperature 293 K, pH 7.4

#	Name	Isotope labeling	Concentration
9	PCP7T	natural abundance	500 uM
10	TRIS	natural abundance	50 mM
11	D2O	natural abundance	100 %

4 3D 1H-13C NOESY

Instrument

Bruker Bruker - 600 MHz

Sample

State isotropic, Solvent system 95% H2O/5% D2O, Pressure 1 atm, Temperature 293 K, pH 7.4

#	Name	Isotope labeling	Concentration
1	PCP7T	[U-100% 13C; U-100% 15N]	350.0 ~ 500.0 uM
2	TRIS	natural abundance	50 mM
3	H2O	natural abundance	95 %
4	D2O	natural abundance	5 %

5 2D DQF-COSY

Instrument

Bruker Bruker - 600 MHz

Sample

State isotropic, Solvent system 95% H2O/5% D2O, Pressure 1 atm, Temperature 293 K, pH 7.4

#	Name	Isotope labeling	Concentration
5	PCP7T	natural abundance	500 uM
6	TRIS	natural abundance	50 mM
7	H2O	natural abundance	95 %
8	D2O	natural abundance	5 %

6 2D 1H-15N HSQC

Instrument

Bruker Bruker - 600 MHz

Sample

State isotropic, Solvent system 95% H2O/5% D2O, Pressure 1 atm, Temperature 293 K, pH 7.4

#	Name	Isotope labeling	Concentration
1	PCP7T	[U-100% 13C; U-100% 15N]	350.0 ~ 500.0 uM
2	TRIS	natural abundance	50 mM
3	H2O	natural abundance	95 %
4	D2O	natural abundance	5 %

7 2D 1H-13C HSQC

Instrument

Bruker Bruker - 600 MHz

Sample

State isotropic, Solvent system 95% H2O/5% D2O, Pressure 1 atm, Temperature 293 K, pH 7.4

#	Name	Isotope labeling	Concentration
1	PCP7T	[U-100% 13C; U-100% 15N]	350.0 ~ 500.0 uM
2	TRIS	natural abundance	50 mM
3	H2O	natural abundance	95 %
4	D2O	natural abundance	5 %

8 3D CBCA(CO)NH

Instrument

Bruker Bruker - 600 MHz

Sample

State isotropic, Solvent system 95% H2O/5% D2O, Pressure 1 atm, Temperature 293 K, pH 7.4

#	Name	Isotope labeling	Concentration
1	PCP7T	[U-100% 13C; U-100% 15N]	350.0 ~ 500.0 uM
2	TRIS	natural abundance	50 mM
3	H2O	natural abundance	95 %
4	D2O	natural abundance	5 %

9 3D HNCO

Instrument

Bruker Bruker - 600 MHz

Sample

State isotropic, Solvent system 95% H2O/5% D2O, Pressure 1 atm, Temperature 293 K, pH 7.4

#	Name	Isotope labeling	Concentration
1	PCP7T	[U-100% 13C; U-100% 15N]	350.0 ~ 500.0 uM
2	TRIS	natural abundance	50 mM
3	H2O	natural abundance	95 %
4	D2O	natural abundance	5 %

10 3D HNCA

Instrument

Bruker Bruker - 600 MHz

Sample

State isotropic, Solvent system 95% H2O/5% D2O, Pressure 1 atm, Temperature 293 K, pH 7.4

#	Name	Isotope labeling	Concentration
1	PCP7T	[U-100% 13C; U-100% 15N]	350.0 ~ 500.0 uM
2	TRIS	natural abundance	50 mM
3	H2O	natural abundance	95 %
4	D2O	natural abundance	5 %

11 3D HNCACB

Instrument

Bruker Bruker - 600 MHz

Sample

State isotropic, Solvent system 95% H2O/5% D2O, Pressure 1 atm, Temperature 293 K, pH 7.4

#	Name	Isotope labeling	Concentration
1	PCP7T	[U-100% 13C; U-100% 15N]	350.0 ~ 500.0 uM
2	TRIS	natural abundance	50 mM
3	H2O	natural abundance	95 %
4	D2O	natural abundance	5 %

12 3D HBHA(CO)NH

Instrument

Bruker Bruker - 600 MHz

Sample

State isotropic, Solvent system 95% H2O/5% D2O, Pressure 1 atm, Temperature 293 K, pH 7.4

#	Name	Isotope labeling	Concentration
1	PCP7T	[U-100% 13C; U-100% 15N]	350.0 ~ 500.0 uM
2	TRIS	natural abundance	50 mM
3	H2O	natural abundance	95 %
4	D2O	natural abundance	5 %

13 3D HN(CO)CA

Instrument

Bruker Bruker - 600 MHz

Sample

State isotropic, Solvent system 95% H2O/5% D2O, Pressure 1 atm, Temperature 293 K, pH 7.4

#	Name	Isotope labeling	Concentration
1	PCP7T	[U-100% 13C; U-100% 15N]	350.0 ~ 500.0 uM
2	TRIS	natural abundance	50 mM
3	H2O	natural abundance	95 %
4	D2O	natural abundance	5 %

14 3D HCCH-TOCSY

Instrument

Bruker Bruker - 600 MHz

Sample

State isotropic, Solvent system 95% H2O/5% D2O, Pressure 1 atm, Temperature 293 K, pH 7.4

#	Name	Isotope labeling	Concentration
1	PCP7T	[U-100% 13C; U-100% 15N]	350.0 ~ 500.0 uM
2	TRIS	natural abundance	50 mM
3	H2O	natural abundance	95 %
4	D2O	natural abundance	5 %

15 3D HNCCCONH

Instrument

Bruker Bruker - 600 MHz

Sample

State isotropic, Solvent system 95% H2O/5% D2O, Pressure 1 atm, Temperature 293 K, pH 7.4

#	Name	Isotope labeling	Concentration
1	PCP7T	[U-100% 13C; U-100% 15N]	350.0 ~ 500.0 uM
2	TRIS	natural abundance	50 mM
3	H2O	natural abundance	95 %
4	D2O	natural abundance	5 %

16 3D CBCANH

Instrument

Bruker Bruker - 600 MHz

Sample

State isotropic, Solvent system 95% H2O/5% D2O, Pressure 1 atm, Temperature 293 K, pH 7.4

#	Name	Isotope labeling	Concentration
1	PCP7T	[U-100% 13C; U-100% 15N]	350.0 ~ 500.0 uM
2	TRIS	natural abundance	50 mM
3	H2O	natural abundance	95 %
4	D2O	natural abundance	5 %

17 2D 1H-1H TOCSY

Instrument

Bruker Bruker - 600 MHz

Sample

State isotropic, Solvent system 95% H2O/5% D2O, Pressure 1 atm, Temperature 293 K, pH 7.4

#	Name	Isotope labeling	Concentration
5	PCP7T	natural abundance	500 uM
6	TRIS	natural abundance	50 mM
7	H2O	natural abundance	95 %
8	D2O	natural abundance	5 %

NMR combined restraints 7 contents Detail

Properties

Input source #1: NMR data (NEF) - Assigned chemical shifts, Distance restraints, Dihedral angle restraints	combined_25064_2mr7.nef
Input source #2: Coordindates	2mr7.cif
Diamagnetism of the molecular assembly	True (excluding Oxygen atoms)
Whether the assembly has a disulfide bond	None
Whether the assembly has a other bond	None
Whether the assembly contains a cyclic polymer	None
Overall data processing status	Warning

Disulfide bonds

None

Other bonds (neither disulfide, covalent nor hydrogen bonds, e.g. Zinc–sulphur bond)

None

Non-standard residues

None

Sequence alignments

Entity_assembly_ID (NMR): A vs Auth_asym_ID (model): A

--------10--------20--------30--------40--------50--------60--------70--------80--------90-
GAMAKAPESATEKVLCALYAEILGVERVGVDDAFHDLGGSSALAMRLIARIREELGVDLPIRQLFSSPTPAGVARALAAKSASWSHPQFEK
|||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
GAMAKAPESATEKVLCALYAEILGVERVGVDDAFHDLGGSSALAMRLIARIREELGVDLPIRQLFSSPTPAGVARALAAKSASWSHPQFEK

Chain assignments

Entity_assembly_ID (NMR)	Auth_asym_ID (model)	Length	Unmapped	Conflict	Sequence coverage (%)
A	A	91	0	0	100.0

Content subtype: combined_25064_2mr7.nef

#	Content subtype	Saveframe	Status	# of rows (sets)	Experiment type	Sequence coverage (%)
1	Assigned chemical shifts	assigned_chem_shift_list_1	OK	292		79.1 (chain: A, length: 91)
2	Assigned chemical shifts	assigned_chem_shift_list_1_2	Warning	502		83.5 (chain: A, length: 91)
3	Assigned chemical shifts	assigned_chem_shift_list_1_2_3	Warning	316		82.4 (chain: A, length: 91)
4	Assigned chemical shifts	assigned_chem_shift_list_1_2_3_4	Warning	437		89.0 (chain: A, length: 91)
1	Distance restraints	CNS/XPLOR_distance_constraints_2	Warning	1369 (1)	noe	84.6 (chain: A, length: 91)
1	Dihedral angle restraints	CNS/XPLOR_dihedral_3	OK	150 (150)	.	97.8 (chain: A, length: 91)

Assigned chemical shifts

Saveframe: assigned_chem_shift_list_1
- Status: OK
- Sequence coverage of experimental data
  - Entity_assembly_ID: A, Chain length: 91, Sequence coverage: 79.1%
- Total number of assignments
  - ¹H chemical shifts: 292
- Completeness of assignments
  - Entity_assemble_ID: A
- Histogram of normalized assigned chemical shifts
- Random coil index and derived parameters (S² and NMR RMSD)
  - Chain_ID: A
Saveframe: assigned_chem_shift_list_1_2
- Status: Warning
- Sequence coverage of experimental data
  - Entity_assembly_ID: A, Chain length: 91, Sequence coverage: 83.5%
- Total number of assignments
  - ¹H chemical shifts: 346
  - ¹³C chemical shifts: 156
- Completeness of assignments
  - Entity_assemble_ID: A
- Redox state of CYS
- Peptide bond of PRO
- Rotameric state of ILE , LEU, and VAL
- Histogram of normalized assigned chemical shifts
- Random coil index and derived parameters (S² and NMR RMSD)
  - Chain_ID: A
Saveframe: assigned_chem_shift_list_1_2_3
- Status: Warning
- Sequence coverage of experimental data
  - Entity_assembly_ID: A, Chain length: 91, Sequence coverage: 82.4%
- Total number of assignments
  - ¹H chemical shifts: 316
- Completeness of assignments
  - Entity_assemble_ID: A
- Histogram of normalized assigned chemical shifts
- Random coil index and derived parameters (S² and NMR RMSD)
  - Chain_ID: A
Saveframe: assigned_chem_shift_list_1_2_3_4
- Status: Warning
- Sequence coverage of experimental data
  - Entity_assembly_ID: A, Chain length: 91, Sequence coverage: 89.0%
- Total number of assignments
  - ¹H chemical shifts: 367
  - ¹⁵N chemical shifts: 70
- Completeness of assignments
  - Entity_assemble_ID: A
- Histogram of normalized assigned chemical shifts
- Random coil index and derived parameters (S² and NMR RMSD)
  - Chain_ID: A

Atom group	# of target shifts	# of assigned shifts	Completeness (%)
¹H chemical shifts	515	292	56.7

Atom group	# of target shifts	# of assigned shifts	Completeness (%)
¹H chemical shifts	184	70	38.0

Atom group	# of target shifts	# of assigned shifts	Completeness (%)
¹H chemical shifts	331	222	67.1

Atom group	# of target shifts	# of assigned shifts	Completeness (%)
¹H chemical shifts	60	54	90.0

Atom group	# of target shifts	# of assigned shifts	Completeness (%)
¹H chemical shifts	29	26	89.7

Atom group	# of target shifts	# of assigned shifts	Completeness (%)
¹H chemical shifts	515	346	67.2
¹³C chemical shifts	397	156	39.3

Atom group	# of target shifts	# of assigned shifts	Completeness (%)
¹H chemical shifts	184	123	66.8
¹³C chemical shifts	182	40	22.0

Atom group	# of target shifts	# of assigned shifts	Completeness (%)
¹H chemical shifts	331	223	67.4
¹³C chemical shifts	215	116	54.0

Atom group	# of target shifts	# of assigned shifts	Completeness (%)
¹H chemical shifts	60	49	81.7
¹³C chemical shifts	60	24	40.0

Atom group	# of target shifts	# of assigned shifts	Completeness (%)
¹H chemical shifts	29	7	24.1
¹³C chemical shifts	28	0	0.0

Atom group	# of target shifts	# of assigned shifts	Completeness (%)
¹H chemical shifts	515	316	61.4

Atom group	# of target shifts	# of assigned shifts	Completeness (%)
¹H chemical shifts	184	122	66.3

Atom group	# of target shifts	# of assigned shifts	Completeness (%)
¹H chemical shifts	331	194	58.6

Atom group	# of target shifts	# of assigned shifts	Completeness (%)
¹H chemical shifts	60	51	85.0

Distance restraints

Saveframe: CNS/XPLOR_distance_constraints_2
- Status: Warning
- Experiment type: noe
- Sequence coverage of experimental data
  - Entity_assembly_ID: A, Chain length: 91, Sequence coverage: 84.6%
- Total number of restraints: 1367
- Weight of restraints: 1.0 (1367)
- Potential type of restraints: square-well-parabolic (1367)
- Range of distance target values: 1.21, 2.75 (Å)
- Histogram of distance target values
- Histogram of discrepancy in distance target values
- Distance restraints per residue
  - Chain_ID: A
- Distance restraints on contact map

Dihedral angle restraints

Saveframe: CNS/XPLOR_dihedral_3
- Status: OK
- Experiment type: .
- Sequence coverage of experimental data
  - Entity_assembly_ID: A, Chain length: 91, Sequence coverage: 97.8%
- Total number of restraints: 150
- Total number of restraints per polymer type: 150
- Weight of restraints: 1.0 (150)
- Potential type of restraints: square-well-parabolic (150)
- Plot of Phi-Psi angle restraints
- Dihedral angle restraints per residue
  - Chain_ID: A

Keywords Non-ribosomal peptide synthetase, peptidyl carrier protein

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Found 1 Document (0.763 seconds)

BMRB: 25064

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Related entities 1. PCP7T, : 1 : 1 : 123 entities Detail

Experiments performed 17 experiments Detail

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NMR combined restraints 7 contents Detail