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BMRB: 25068

2MRC

NMR Structure and 1H, 13C and 15N Chemical Shift Assignments for High mobility group protein from Plasmodium falciparum 3D7.

Authors

Tang, C., Barnwal, R., Varani, G.

Assembly

High mobility group protein from Plasmodium falciparum 3D7

Entity

1. High mobility group protein from Plasmodium falciparum 3D7 (polymer, Thiol state: not present), 92 monomers, 10834.47 Da Detail

MAHHHHHHMK KKDPLAPKRA LSAYMFYVKD KRLEIIKEKP ELAKDVAQVG KLIGEAWGQL SPAQKAPYEK KAQLDKVRYS KEIEEYRKKN QE

Formula weight

10834.47 Da

Source organism

Plasmodium falciparum 3D7

Exptl. method

solution NMR

Refine. method

torsion angle dynamics

Data set

assigned_chemical_shifts

Chem. Shift Complete

Sequence coverage: 87.0 %, Completeness: 74.7 %, Completeness (bb): 85.4 % Detail

Polymer type: polypeptide(L)

	Total	¹H	¹³C	¹⁵N
All	74.7 % (867 of 1160)	69.2 % (430 of 621)	79.8 % (355 of 445)	87.2 % (82 of 94)
Backbone	85.4 % (463 of 542)	84.6 % (154 of 182)	85.7 % (234 of 273)	86.2 % (75 of 87)
Sidechain	67.9 % (480 of 707)	62.9 % (276 of 439)	75.5 % (197 of 261)	100.0 % (7 of 7)
Aromatic	11.6 % (10 of 86)	11.6 % (5 of 43)	9.5 % (4 of 42)	100.0 % (1 of 1)
Methyl	96.2 % (77 of 80)	95.0 % (38 of 40)	97.5 % (39 of 40)

1. entity

MAHHHHHHMK KKDPLAPKRA LSAYMFYVKD KRLEIIKEKP ELAKDVAQVG KLIGEAWGQL SPAQKAPYEK KAQLDKVRYS KEIEEYRKKN QE

Show sample condition

Sample

Solvent system 93% H2O/7% D2O, Pressure 1 atm, Temperature 298 K, pH 7.0

#	Name	Isotope labeling	Concentration
1	High mobility group protein	[U-95% 13C; U-95% 15N]	0.9 mM
2	H2O	natural abundance	93 %
3	D20	natural abundance	7 %

LACS Plot; CA

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹H	DSS	methyl protons	internal	direct	1.0
¹³C	DSS	methyl protons	na	indirect	0.2514495
¹⁵N	DSS	methyl protons	na	indirect	0.1013291

Referencing offset: -1.02 ppm, Outliers: 2 Detail

LACS Plot; CB

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹H	DSS	methyl protons	internal	direct	1.0
¹³C	DSS	methyl protons	na	indirect	0.2514495
¹⁵N	DSS	methyl protons	na	indirect	0.1013291

Referencing offset: -1.02 ppm, Outliers: 2 Detail

LACS Plot; HA

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹H	DSS	methyl protons	internal	direct	1.0
¹³C	DSS	methyl protons	na	indirect	0.2514495
¹⁵N	DSS	methyl protons	na	indirect	0.1013291

Referencing offset: 0.15 ppm, Outliers: 1 Detail

LACS Plot; CO

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹H	DSS	methyl protons	internal	direct	1.0
¹³C	DSS	methyl protons	na	indirect	0.2514495
¹⁵N	DSS	methyl protons	na	indirect	0.1013291

Referencing offset: -0.31 ppm, Outliers: 2 Detail

Protein Blocks Logo

Calculated from 10 models in PDB: 2MRC, Strand ID: A Detail

Release date

2014-07-20

Related entities 1. High mobility group protein from Plasmodium falciparum 3D7, : 1 : 274 entities Detail

Experiments performed 12 experiments Detail

1 2D 1H-15N HSQC