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BMRB: 25070

2MRE

NMR structure of the Rad18-UBZ/ubiquitin complex

Authors

Rizzo, A.A., Salerno, P.E., Bezsonova, I., Korzhnev, D.M.

Assembly

Rad18-UBZ/ubiquitin complex

Entity

1. Ubiquitin (polymer, Thiol state: not present), 79 monomers, 8845.999 Da Detail

GSHMQIFVKT LTGKTITLEV EPSDTIENVK AKIQDKEGIP PDQQRLIFAG KQLEDGRTLS DYNIQKESTL HLVLRLRGG

2. Ubiquitin-binding zinc finger (UBZ) domain from human Rad18 (polymer, Thiol state: all other bound), 33 monomers, 3622.074 Da Detail

GSRQVTKVDC PVCGVNIPES HINKHLDSCL SRE

3. ZINC ION (non-polymer), 65.409 Da

Total weight

12533.482 Da

Max. entity weight

8845.999 Da

Source organism

Homo sapiens

Exptl. method

solution NMR

Refine. method

simulated annealing

Data set

assigned_chemical_shifts

Chem. Shift Complete

Sequence coverage: 93.8 %, Completeness: 88.1 %, Completeness (bb): 90.5 % Detail

Polymer type: polypeptide(L)

	Total	¹H	¹³C	¹⁵N
All	88.1 % (1144 of 1298)	87.7 % (597 of 681)	88.2 % (440 of 499)	90.7 % (107 of 118)
Backbone	90.5 % (599 of 662)	89.9 % (205 of 228)	90.5 % (296 of 327)	91.6 % (98 of 107)
Sidechain	86.9 % (642 of 739)	86.5 % (392 of 453)	87.6 % (241 of 275)	81.8 % (9 of 11)
Aromatic	4.5 % (2 of 44)	4.5 % (1 of 22)	4.5 % (1 of 22)
Methyl	99.2 % (131 of 132)	100.0 % (66 of 66)	98.5 % (65 of 66)

1. Ubiquitin

GSHMQIFVKT LTGKTITLEV EPSDTIENVK AKIQDKEGIP PDQQRLIFAG KQLEDGRTLS DYNIQKESTL HLVLRLRGG

2. Ubiquitin-binding zinc finger (UBZ) domain from human Rad18

GSRQVTKVDC PVCGVNIPES HINKHLDSCL SRE

Show sample condition

Sample #1

Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 273 K, pH 6.5, Details UBZ concentrations are inexact since it has no aromatics

#	Name	Isotope labeling	Concentration
1	Ubiquitin-binding zinc finger (UBZ) domain from human Rad18	[U-13C; U-15N]	0.5 mM
2	Ubiquitin	natural abundance	5 mM
3	MOPS	natural abundance	25 mM
4	NaCl	natural abundance	50 mM
5	ZnCl2	natural abundance	0.05 mM
6	H2O	natural abundance	90 %
7	D2O	natural abundance	10 %

Sample #2

Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 273 K, pH 6.5, Details UBZ concentrations are inexact since it has no aromatics

#	Name	Isotope labeling	Concentration
8	Ubiquitin	[U-13C; U-15N]	2 mM
9	Ubiquitin-binding zinc finger (UBZ) domain from human Rad18	natural abundance	7.0 ~ 10.0 mM
10	MOPS	natural abundance	25 mM
11	NaCl	natural abundance	50 mM
12	ZnCl2	natural abundance	0.05 mM
13	H2O	natural abundance	90 %
14	D2O	natural abundance	10 %

Protein Blocks Logo

Calculated from 20 models in PDB: 2MRE, Strand ID: A, B Detail

Release date

2014-09-18

Citation

NMR structure of the human Rad18 zinc finger in complex with ubiquitin defines a class of UBZ domains in proteins linked to the DNA damage response
Rizzo, A.A., Salerno, P.E., Bezsonova, I., Korzhnev, D.M.
Biochemistry (2014), 53, 5895-5906, PubMed 25162118 , DOI 10.1021/bi500823h , Abstract

Entries sharing articles BMRB: 1 entries Detail

BMRB: 25071 released on 2014-09-18
Title NMR structure of the ubiquitin-binding zinc finger (UBZ) domain from human Rad18

Related entities 1. Ubiquitin, : 138 : 188 entities Detail

Related entities 2. Ubiquitin-binding zinc finger (UBZ) domain from human Rad18, : 1 : 1 : 1 : 25 entities Detail

Interaction partners 2. Ubiquitin-binding zinc finger (UBZ) domain from human Rad18, : 28 interactors Detail

More details for 28 interactors identified by 13 citations

Experiments performed 16 experiments Detail

1 2D 1H-15N HSQC