BMRBj - BMREntryMaster

Found 1 Document (0.523 seconds)

BMRB: 25086

2MRN

truncated EcMazE

Authors

Zorzini, V., Buts, L., Loris, R., van Nuland, N.A.J.

Assembly

truncated EcMazE

Entity

1. truncated EcMazE (polymer, Thiol state: not present), 67 monomers, 7658.564 × 2 Da Detail

NHKVHHHHHH MSDDDDKGIH SSVKRWGNSP AVRIPATLMQ ALNLNIDDEV KIDLVDGKLI IEPVRKE

Total weight

15317.128 Da

Max. entity weight

7658.564 Da

Source organism

Escherichia coli

Exptl. method

solution NMR

Refine. method

simulated annealing

Data set

assigned_chemical_shifts

Chem. Shift Complete

Sequence coverage: 94.0 %, Completeness: 88.3 %, Completeness (bb): 89.6 % Detail

Polymer type: polypeptide(L)

	Total	¹H	¹³C	¹⁵N
All	88.3 % (689 of 780)	88.0 % (358 of 407)	88.4 % (268 of 303)	90.0 % (63 of 70)
Backbone	89.6 % (355 of 396)	88.8 % (119 of 134)	90.4 % (179 of 198)	89.1 % (57 of 64)
Sidechain	87.9 % (394 of 448)	87.5 % (239 of 273)	88.2 % (149 of 169)	100.0 % (6 of 6)
Aromatic	29.5 % (13 of 44)	31.8 % (7 of 22)	23.8 % (5 of 21)	100.0 % (1 of 1)
Methyl	98.7 % (75 of 76)	97.4 % (37 of 38)	100.0 % (38 of 38)

1. truncEcMazE

NHKVHHHHHH MSDDDDKGIH SSVKRWGNSP AVRIPATLMQ ALNLNIDDEV KIDLVDGKLI IEPVRKE

Show sample condition

Sample

Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 298 K, pH 6.5

#	Name	Isotope labeling	Concentration
1	truncEcMazE	[U-99% 13C; U-99% 15N]	1 mM
2	sodium phosphate	natural abundance	50 mM
3	sodium chloride	natural abundance	50 mM
4	H2O	natural abundance	90 %
5	D2O	natural abundance	10 %

LACS Plot; CA

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹H	DSS	methyl protons	internal	direct	1.0
¹³C	DSS	methyl protons	na	indirect	0.2514495
¹⁵N	DSS	methyl protons	na	indirect	0.1013291

Referencing offset: -0.18 ppm, Outliers: 1 Detail

LACS Plot; CB

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹H	DSS	methyl protons	internal	direct	1.0
¹³C	DSS	methyl protons	na	indirect	0.2514495
¹⁵N	DSS	methyl protons	na	indirect	0.1013291

Referencing offset: -0.18 ppm, Outliers: 1 Detail

LACS Plot; HA

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹H	DSS	methyl protons	internal	direct	1.0
¹³C	DSS	methyl protons	na	indirect	0.2514495
¹⁵N	DSS	methyl protons	na	indirect	0.1013291

Referencing offset: -0.05 ppm, Outliers: 5 Detail

LACS Plot; CO

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹H	DSS	methyl protons	internal	direct	1.0
¹³C	DSS	methyl protons	na	indirect	0.2514495
¹⁵N	DSS	methyl protons	na	indirect	0.1013291

Referencing offset: 0.28 ppm, Outliers: 2 Detail

Protein Blocks Logo

Calculated from 20 models in PDB: 2MRN, Strand ID: A, B Detail

Release date

2015-02-01

Citation

Escherichia coli antitoxin MazE as transcription factor: insights into MazE-DNA binding
Zorzini, V., Buts, L., Schrank, E., Sterckx, Y.G.J., Respondek, M., Engelberg-Kulka, H., Loris, R., Zangger, K., van Nuland, N.A.J.
Nucleic Acids Res. (2015), 43, 1241-1256, PubMed 25564525 , DOI 10.1093/nar/gku1352 , Abstract

Related entities 1. truncated EcMazE, : 1 : 1 entities Detail

Experiments performed 12 experiments Detail

1 2D 1H-15N HSQC