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Resonance assignments of myristoylated Y28F/Y67F mutant of the Mason-Pfizer monkey virus matrix protein
Authors
Dolezal, M., Hrabal, R.
Assembly
MPMV MA Y28F Y67F
Entity
1. MPMV MA Y28F Y67F (polymer, Thiol state: all free), 125 monomers, 14867.79 Da Detail

XGQELSQHER YVEQLKQALK TRGVKVKFAD LLKFFDFVKD TCPWFPQEGT IDIKRWRRVG DCFQDYFNTF GPEKVPVTAF SYWNLIKELI DKKEVNPQVM AAVAQTEEIL KSNSQTDLEH HHHHH


Formula weight
14867.79 Da
Source organism
Mason-Pfizer monkey virus
Exptl. method
solution NMR
Refine. method
torsion angle dynamics (Cyana 2.1), distance geometry dynamics, explicit water (YASARA 13.3.26)
Data set
assigned_chemical_shifts
Chem. Shift Complete
Sequence coverage: 96.0 %, Completeness: 91.1 %, Completeness (bb): 94.3 % Detail

Polymer type: polypeptide(L)

Total1H13C15N
All91.1 % (1402 of 1539)91.6 % (737 of 805)89.1 % (534 of 599)97.0 % (131 of 135)
Backbone94.3 % (692 of 734)92.7 % (230 of 248)94.6 % (347 of 367)96.6 % (115 of 119)
Sidechain89.1 % (823 of 924)91.0 % (507 of 557)85.5 % (300 of 351)100.0 % (16 of 16)
Aromatic69.7 % (124 of 178)83.1 % (74 of 89)54.7 % (47 of 86)100.0 % (3 of 3)
Methyl95.9 % (117 of 122)93.4 % (57 of 61)98.4 % (60 of 61)

1. MPMV MA Y28F Y67F

XGQELSQHER YVEQLKQALK TRGVKVKFAD LLKFFDFVKD TCPWFPQEGT IDIKRWRRVG DCFQDYFNTF GPEKVPVTAF SYWNLIKELI DKKEVNPQVM AAVAQTEEIL KSNSQTDLEH HHHHH

Sample #1

Solvent system 95% H2O/5% D2O, Pressure 1.000 atm, Temperature 298.000 K, pH 6.000, Details the myristoyl residue is reverse-labeled


#NameIsotope labelingTypeConcentration
1MPMV_MA_Y28F_Y67F[U-99% 13C; U-99% 15N; NA-MYR]1.0 mM
2NaClnatural abundance300.0 mM
3TCEPnatural abundance2.5 mM
4sodium phosphatenatural abundance50.0 mM
5H2Onatural abundance95 %
6D2Onatural abundance5 %
Sample #2

Solvent system 95% H2O/5% D2O, Pressure 1.000 atm, Temperature 298.000 K, pH 6.000, Details the myristoyl residue and all histidine residues are reverse-labeled


#NameIsotope labelingTypeConcentration
7MPMV_MA_Y28F_Y67F[U-99% 13C; U-99% 15N; NA-MYR,H]1.0 mM
8NaClnatural abundance300.0 mM
9TCEPnatural abundance2.5 mM
10sodium phosphatenatural abundance50.0 mM
11H2Onatural abundance95 %
12D2Onatural abundance5 %
Sample #3

Solvent system 95% H2O/5% D2O, Pressure 1.000 atm, Temperature 298.000 K, pH 6.000


#NameIsotope labelingTypeConcentration
13MPMV_MA_Y28F_Y67F[U-99% 13C; U-99% 15N]1.0 mM
14NaClnatural abundance300.0 mM
15TCEPnatural abundance2.5 mM
16sodium phosphatenatural abundance50.0 mM
17H2Onatural abundance95 %
18D2Onatural abundance5 %
Sample #4

Solvent system 95% H2O/5% D2O, Pressure 1.000 atm, Temperature 298.000 K, pH 6.000, Details aminoacid residues are partially reverse-labeled


#NameIsotope labelingTypeConcentration
19MPMV_MA_Y28F_Y67F[U-99% 13C]-MYR1.0 mM
20NaClnatural abundance300.0 mM
21TCEPnatural abundance2.5 mM
22sodium phosphatenatural abundance50.0 mM
23H2Onatural abundance95 %
24D2Onatural abundance5 %

LACS Plot; CA
Referencing offset: -0.05 ppm, Outliers: 4 Detail
LACS Plot; CB
Referencing offset: -0.05 ppm, Outliers: 4 Detail
LACS Plot; HA
Referencing offset: 0.01 ppm, Outliers: 2 Detail
LACS Plot; CO
Referencing offset: -0.32 ppm, Outliers: 3 Detail
Protein Blocks Logo
Calculated from 40 models in PDB: 2MV4, Strand ID: A Detail


Release date
2015-03-23
Citation
Resonance assignments of the myristoylated Y28F/Y67F mutant of the Mason-Pfizer monkey virus matrix protein
Dolezal, M., Hrabal, R., Ruml, T., Rumlova, M.
Biomol. NMR Assign. (2015), 9, 229-233, PubMed 25773138 , DOI 10.1007/s12104-014-9580-0 ,
Related entities 1. MPMV MA Y28F Y67F, : 1 : 23 entities Detail
Interaction partners 1. MPMV MA Y28F Y67F, : 1 interactors Detail
Experiments performed 27 experiments Detail
nullKeywords isotopic labeling, matrix protein, M-PMV, Mason-Pfizer monkey virus, myristoylation, resonance assignment, reverse labeling