Solution NMR structure of tRNApro:MLV Nucleocapsid Protein (1:1) Complex
Polymer type: polypeptide(L) polyribonucleotide
Total | 1H | 13C | |
---|---|---|---|
All | 5.9 % (99 of 1674) | 10.0 % (97 of 969) | 0.3 % (2 of 705) |
Backbone | 3.6 % (38 of 1051) | 6.7 % (36 of 537) | 0.4 % (2 of 514) |
Sidechain | 9.1 % (61 of 672) | 14.1 % (61 of 432) | 0.0 % (0 of 240) |
Aromatic | 8.1 % (24 of 298) | 14.0 % (24 of 171) | 0.0 % (0 of 127) |
Methyl | 0.0 % (0 of 26) | 0.0 % (0 of 13) | 0.0 % (0 of 13) |
1. MLV Nucleocapsid
ATVVSGQKQD RQGGERRRSQ LDRDQCAYCK EKGHWAKDCP KKPRGPRGPR PQTSL2. tRNApro
GGCUCGUUGG UCUAGGGGUA UGAUUCUCGC UUAGGGUGCG AGAGGUCCCG GGUUCAAAUC CCGGACGAGC CSolvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 311 K, pH 7.2
# | Name | Isotope labeling | Type | Concentration |
---|---|---|---|---|
1 | NC-tRNApro (1:1) | natural abundance | 0.5 mM | |
2 | MgCl2 | natural abundance | 1 mM | |
3 | NaCl | natural abundance | 10 mM | |
4 | Tris | natural abundance | 10 mM | |
5 | H2O | natural abundance | 90 % | |
6 | D2O | natural abundance | 10 % |
Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 311 K, pH 7.2
# | Name | Isotope labeling | Type | Concentration |
---|---|---|---|---|
7 | NC-tRNApro (1:1) | 13C, 15N G-lab tRNA-pro | 0.5 mM | |
8 | MgCl2 | natural abundance | 1 mM | |
9 | NaCl | natural abundance | 10 mM | |
10 | Tris | natural abundance | 10 mM | |
11 | H2O | natural abundance | 90 % | |
12 | D2O | natural abundance | 10 % |
Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 311 K, pH 7.2
# | Name | Isotope labeling | Type | Concentration |
---|---|---|---|---|
13 | NC-tRNApro (1:1) | 13C, 15N G-lab tRNA-pro13C | 0.5 mM | |
14 | MgCl2 | natural abundance | 1 mM | |
15 | NaCl | natural abundance | 10 mM | |
16 | Tris | natural abundance | 10 mM | |
17 | H2O | natural abundance | 90 % | |
18 | D2O | natural abundance | 10 % |
Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 311 K, pH 7.2
# | Name | Isotope labeling | Type | Concentration |
---|---|---|---|---|
19 | NC-tRNApro (1:1) | 13C, 15N G-lab tRNA-pro | 0.5 mM | |
20 | MgCl2 | natural abundance | 1 mM | |
21 | NaCl | natural abundance | 10 mM | |
22 | Tris | natural abundance | 10 mM | |
23 | H2O | natural abundance | 90 % | |
24 | D2O | natural abundance | 10 % |
Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 311 K, pH 7.2
# | Name | Isotope labeling | Type | Concentration |
---|---|---|---|---|
25 | NC-tRNApro (1:1) | 13C, 15N G-lab tRNA-13C | 0.5 mM | |
26 | MgCl2 | natural abundance | 1 mM | |
27 | NaCl | natural abundance | 10 mM | |
28 | Tris | natural abundance | 10 mM | |
29 | H2O | natural abundance | 90 % | |
30 | D2O | natural abundance | 10 % |
Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 311 K, pH 7.2
# | Name | Isotope labeling | Type | Concentration |
---|---|---|---|---|
31 | NC-tRNApro (1:1) | 13C, 15N G-lab tRNA-pro | 0.5 mM | |
32 | MgCl2 | natural abundance | 1 mM | |
33 | NaCl | natural abundance | 10 mM | |
34 | Tris | natural abundance | 10 mM | |
35 | H2O | natural abundance | 90 % | |
36 | D2O | natural abundance | 10 % |
Polymer type: polypeptide(L) polyribonucleotide
Total | 1H | 13C | |
---|---|---|---|
All | 7.0 % (236 of 3348) | 11.9 % (230 of 1938) | 0.4 % (6 of 1410) |
Backbone | 4.5 % (94 of 2102) | 8.2 % (88 of 1074) | 0.6 % (6 of 1028) |
Sidechain | 10.7 % (144 of 1344) | 16.6 % (143 of 864) | 0.2 % (1 of 480) |
Aromatic | 17.3 % (103 of 596) | 30.1 % (103 of 342) | 0.0 % (0 of 254) |
Methyl | 0.0 % (0 of 52) | 0.0 % (0 of 26) | 0.0 % (0 of 26) |
1. MLV Nucleocapsid
ATVVSGQKQD RQGGERRRSQ LDRDQCAYCK EKGHWAKDCP KKPRGPRGPR PQTSL2. tRNApro
GGCUCGUUGG UCUAGGGGUA UGAUUCUCGC UUAGGGUGCG AGAGGUCCCG GGUUCAAAUC CCGGACGAGC CSolvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 311 K, pH 7.2
# | Name | Isotope labeling | Type | Concentration |
---|---|---|---|---|
1 | NC-tRNApro (1:1) | natural abundance | 0.5 mM | |
2 | MgCl2 | natural abundance | 1 mM | |
3 | NaCl | natural abundance | 10 mM | |
4 | Tris | natural abundance | 10 mM | |
5 | H2O | natural abundance | 90 % | |
6 | D2O | natural abundance | 10 % |
Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 311 K, pH 7.2
# | Name | Isotope labeling | Type | Concentration |
---|---|---|---|---|
7 | NC-tRNApro (1:1) | 13C, 15N G-lab tRNA-pro | 0.5 mM | |
8 | MgCl2 | natural abundance | 1 mM | |
9 | NaCl | natural abundance | 10 mM | |
10 | Tris | natural abundance | 10 mM | |
11 | H2O | natural abundance | 90 % | |
12 | D2O | natural abundance | 10 % |
Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 311 K, pH 7.2
# | Name | Isotope labeling | Type | Concentration |
---|---|---|---|---|
13 | NC-tRNApro (1:1) | 13C, 15N G-lab tRNA-pro13C | 0.5 mM | |
14 | MgCl2 | natural abundance | 1 mM | |
15 | NaCl | natural abundance | 10 mM | |
16 | Tris | natural abundance | 10 mM | |
17 | H2O | natural abundance | 90 % | |
18 | D2O | natural abundance | 10 % |
Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 311 K, pH 7.2
# | Name | Isotope labeling | Type | Concentration |
---|---|---|---|---|
19 | NC-tRNApro (1:1) | 13C, 15N G-lab tRNA-pro | 0.5 mM | |
20 | MgCl2 | natural abundance | 1 mM | |
21 | NaCl | natural abundance | 10 mM | |
22 | Tris | natural abundance | 10 mM | |
23 | H2O | natural abundance | 90 % | |
24 | D2O | natural abundance | 10 % |
Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 311 K, pH 7.2
# | Name | Isotope labeling | Type | Concentration |
---|---|---|---|---|
25 | NC-tRNApro (1:1) | 13C, 15N G-lab tRNA-13C | 0.5 mM | |
26 | MgCl2 | natural abundance | 1 mM | |
27 | NaCl | natural abundance | 10 mM | |
28 | Tris | natural abundance | 10 mM | |
29 | H2O | natural abundance | 90 % | |
30 | D2O | natural abundance | 10 % |
Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 311 K, pH 7.2
# | Name | Isotope labeling | Type | Concentration |
---|---|---|---|---|
31 | NC-tRNApro (1:1) | 13C, 15N G-lab tRNA-pro | 0.5 mM | |
32 | MgCl2 | natural abundance | 1 mM | |
33 | NaCl | natural abundance | 10 mM | |
34 | Tris | natural abundance | 10 mM | |
35 | H2O | natural abundance | 90 % | |
36 | D2O | natural abundance | 10 % |
Polymer type: polypeptide(L) polyribonucleotide
Total | 1H | 13C | |
---|---|---|---|
All | 5.7 % (284 of 5022) | 8.2 % (237 of 2907) | 2.2 % (47 of 2115) |
Backbone | 3.7 % (117 of 3153) | 5.6 % (91 of 1611) | 1.7 % (26 of 1542) |
Sidechain | 8.5 % (171 of 2016) | 11.3 % (147 of 1296) | 3.3 % (24 of 720) |
Aromatic | 13.6 % (122 of 894) | 20.1 % (103 of 513) | 5.0 % (19 of 381) |
Methyl | 0.0 % (0 of 78) | 0.0 % (0 of 39) | 0.0 % (0 of 39) |
1. MLV Nucleocapsid
ATVVSGQKQD RQGGERRRSQ LDRDQCAYCK EKGHWAKDCP KKPRGPRGPR PQTSL2. tRNApro
GGCUCGUUGG UCUAGGGGUA UGAUUCUCGC UUAGGGUGCG AGAGGUCCCG GGUUCAAAUC CCGGACGAGC CSolvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 311 K, pH 7.2
# | Name | Isotope labeling | Type | Concentration |
---|---|---|---|---|
1 | NC-tRNApro (1:1) | natural abundance | 0.5 mM | |
2 | MgCl2 | natural abundance | 1 mM | |
3 | NaCl | natural abundance | 10 mM | |
4 | Tris | natural abundance | 10 mM | |
5 | H2O | natural abundance | 90 % | |
6 | D2O | natural abundance | 10 % |
Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 311 K, pH 7.2
# | Name | Isotope labeling | Type | Concentration |
---|---|---|---|---|
7 | NC-tRNApro (1:1) | 13C, 15N G-lab tRNA-pro | 0.5 mM | |
8 | MgCl2 | natural abundance | 1 mM | |
9 | NaCl | natural abundance | 10 mM | |
10 | Tris | natural abundance | 10 mM | |
11 | H2O | natural abundance | 90 % | |
12 | D2O | natural abundance | 10 % |
Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 311 K, pH 7.2
# | Name | Isotope labeling | Type | Concentration |
---|---|---|---|---|
13 | NC-tRNApro (1:1) | 13C, 15N G-lab tRNA-pro13C | 0.5 mM | |
14 | MgCl2 | natural abundance | 1 mM | |
15 | NaCl | natural abundance | 10 mM | |
16 | Tris | natural abundance | 10 mM | |
17 | H2O | natural abundance | 90 % | |
18 | D2O | natural abundance | 10 % |
Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 311 K, pH 7.2
# | Name | Isotope labeling | Type | Concentration |
---|---|---|---|---|
19 | NC-tRNApro (1:1) | 13C, 15N G-lab tRNA-pro | 0.5 mM | |
20 | MgCl2 | natural abundance | 1 mM | |
21 | NaCl | natural abundance | 10 mM | |
22 | Tris | natural abundance | 10 mM | |
23 | H2O | natural abundance | 90 % | |
24 | D2O | natural abundance | 10 % |
Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 311 K, pH 7.2
# | Name | Isotope labeling | Type | Concentration |
---|---|---|---|---|
25 | NC-tRNApro (1:1) | 13C, 15N G-lab tRNA-13C | 0.5 mM | |
26 | MgCl2 | natural abundance | 1 mM | |
27 | NaCl | natural abundance | 10 mM | |
28 | Tris | natural abundance | 10 mM | |
29 | H2O | natural abundance | 90 % | |
30 | D2O | natural abundance | 10 % |
Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 311 K, pH 7.2
# | Name | Isotope labeling | Type | Concentration |
---|---|---|---|---|
31 | NC-tRNApro (1:1) | 13C, 15N G-lab tRNA-pro | 0.5 mM | |
32 | MgCl2 | natural abundance | 1 mM | |
33 | NaCl | natural abundance | 10 mM | |
34 | Tris | natural abundance | 10 mM | |
35 | H2O | natural abundance | 90 % | |
36 | D2O | natural abundance | 10 % |
Polymer type: polypeptide(L) polyribonucleotide
Total | 1H | 13C | |
---|---|---|---|
All | 4.8 % (319 of 6696) | 6.9 % (267 of 3876) | 1.8 % (52 of 2820) |
Backbone | 3.5 % (146 of 4204) | 5.4 % (115 of 2148) | 1.5 % (31 of 2056) |
Sidechain | 6.7 % (179 of 2688) | 8.9 % (153 of 1728) | 2.7 % (26 of 960) |
Aromatic | 10.7 % (128 of 1192) | 15.9 % (109 of 684) | 3.7 % (19 of 508) |
Methyl | 0.0 % (0 of 104) | 0.0 % (0 of 52) | 0.0 % (0 of 52) |
1. MLV Nucleocapsid
ATVVSGQKQD RQGGERRRSQ LDRDQCAYCK EKGHWAKDCP KKPRGPRGPR PQTSL2. tRNApro
GGCUCGUUGG UCUAGGGGUA UGAUUCUCGC UUAGGGUGCG AGAGGUCCCG GGUUCAAAUC CCGGACGAGC CSolvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 311 K, pH 7.2
# | Name | Isotope labeling | Type | Concentration |
---|---|---|---|---|
1 | NC-tRNApro (1:1) | natural abundance | 0.5 mM | |
2 | MgCl2 | natural abundance | 1 mM | |
3 | NaCl | natural abundance | 10 mM | |
4 | Tris | natural abundance | 10 mM | |
5 | H2O | natural abundance | 90 % | |
6 | D2O | natural abundance | 10 % |
Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 311 K, pH 7.2
# | Name | Isotope labeling | Type | Concentration |
---|---|---|---|---|
7 | NC-tRNApro (1:1) | 13C, 15N G-lab tRNA-pro | 0.5 mM | |
8 | MgCl2 | natural abundance | 1 mM | |
9 | NaCl | natural abundance | 10 mM | |
10 | Tris | natural abundance | 10 mM | |
11 | H2O | natural abundance | 90 % | |
12 | D2O | natural abundance | 10 % |
Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 311 K, pH 7.2
# | Name | Isotope labeling | Type | Concentration |
---|---|---|---|---|
13 | NC-tRNApro (1:1) | 13C, 15N G-lab tRNA-pro13C | 0.5 mM | |
14 | MgCl2 | natural abundance | 1 mM | |
15 | NaCl | natural abundance | 10 mM | |
16 | Tris | natural abundance | 10 mM | |
17 | H2O | natural abundance | 90 % | |
18 | D2O | natural abundance | 10 % |
Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 311 K, pH 7.2
# | Name | Isotope labeling | Type | Concentration |
---|---|---|---|---|
19 | NC-tRNApro (1:1) | 13C, 15N G-lab tRNA-pro | 0.5 mM | |
20 | MgCl2 | natural abundance | 1 mM | |
21 | NaCl | natural abundance | 10 mM | |
22 | Tris | natural abundance | 10 mM | |
23 | H2O | natural abundance | 90 % | |
24 | D2O | natural abundance | 10 % |
Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 311 K, pH 7.2
# | Name | Isotope labeling | Type | Concentration |
---|---|---|---|---|
25 | NC-tRNApro (1:1) | 13C, 15N G-lab tRNA-13C | 0.5 mM | |
26 | MgCl2 | natural abundance | 1 mM | |
27 | NaCl | natural abundance | 10 mM | |
28 | Tris | natural abundance | 10 mM | |
29 | H2O | natural abundance | 90 % | |
30 | D2O | natural abundance | 10 % |
Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 311 K, pH 7.2
# | Name | Isotope labeling | Type | Concentration |
---|---|---|---|---|
31 | NC-tRNApro (1:1) | 13C, 15N G-lab tRNA-pro | 0.5 mM | |
32 | MgCl2 | natural abundance | 1 mM | |
33 | NaCl | natural abundance | 10 mM | |
34 | Tris | natural abundance | 10 mM | |
35 | H2O | natural abundance | 90 % | |
36 | D2O | natural abundance | 10 % |