BMRBj - BMREntryMaster

	Total	¹H	¹³C	¹⁵N
All	95.2 % (1141 of 1199)	97.1 % (602 of 620)	91.7 % (432 of 471)	99.1 % (107 of 108)
Backbone	95.8 % (577 of 602)	99.0 % (201 of 203)	92.7 % (280 of 302)	99.0 % (96 of 97)
Sidechain	95.3 % (662 of 695)	96.2 % (401 of 417)	93.6 % (250 of 267)	100.0 % (11 of 11)
Aromatic	77.8 % (98 of 126)	79.4 % (50 of 63)	75.4 % (46 of 61)	100.0 % (2 of 2)
Methyl	100.0 % (112 of 112)	100.0 % (56 of 56)	100.0 % (56 of 56)

1. MAVS CARD

GSMPFAEDKT YKYICRNFSN FCNVDVVEIL PYLPCLTARD QDRLRATCTL SGNRDTLWHL FNTLQRRPGW VEYFIAALRG CELVDLADEV ASVYQSYQPR TS

Show sample condition

Sample

Solvent system 93% H2O/7% D2O, Pressure 1 atm, Temperature 298 K, pH 3, Details 0.3 mM uniformly 13C, 15N-labled MAVS-CARD in 50 mM sodium phosphate buffer with 7% D2O and 1 mM DTT at pH 3.0

#	Name	Isotope labeling	Concentration
1	MAVS CARD	[U-99% 13C; U-99% 15N]	300 uM
2	sodium phosphate buffer	natural abundance	50 mM
3	DTT	natural abundance	1 mM

LACS Plot; CA

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹³C	DSS	methyl protons	internal	indirect	0.2514495
¹H	DSS	methyl protons	internal	direct	1.0
¹⁵N	DSS	methyl protons	internal	indirect	0.1013291

Referencing offset: -0.32 ppm, Outliers: 2 Detail

LACS Plot; CB

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹³C	DSS	methyl protons	internal	indirect	0.2514495
¹H	DSS	methyl protons	internal	direct	1.0
¹⁵N	DSS	methyl protons	internal	indirect	0.1013291

Referencing offset: -0.32 ppm, Outliers: 2 Detail

LACS Plot; HA

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹³C	DSS	methyl protons	internal	indirect	0.2514495
¹H	DSS	methyl protons	internal	direct	1.0
¹⁵N	DSS	methyl protons	internal	indirect	0.1013291

Referencing offset: 0.04 ppm, Outliers: 1 Detail

LACS Plot; CO

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹³C	DSS	methyl protons	internal	indirect	0.2514495
¹H	DSS	methyl protons	internal	direct	1.0
¹⁵N	DSS	methyl protons	internal	indirect	0.1013291

Referencing offset: -0.12 ppm, Outliers: 1 Detail

Protein Blocks Logo

Calculated from 20 models in PDB: 2MS8, Strand ID: A Detail

Release date

2015-08-30

Citation

High-resolution solid-state NMR structure of the helical signal transduction filament MAVS CARD
He, L., Bardiaux, B., Spehr, J., Luehrs, T., Ritter, C.
Not known

Related entities 1. MAVS CARD, : 1 : 1 : 6 : 9 entities Detail

Interaction partners 1. MAVS CARD, : 53 interactors Detail

More details for 53 interactors identified by 24 citations

Experiments performed 12 experiments Detail

1 2D 1H-15N HSQC

Instrument

Bruker Avance - 600 MHz

Sample

State isotropic, Solvent system 93% H2O/7% D2O, Pressure 1 atm, Temperature 298 K, pH 3, Details 0.3 mM uniformly 13C, 15N-labled MAVS-CARD in 50 mM sodium phosphate buffer with 7% D2O and 1 mM DTT at pH 3.0

#	Name	Isotope labeling	Concentration
1	MAVS CARD	[U-99% 13C; U-99% 15N]	300 uM
2	sodium phosphate buffer	natural abundance	50 mM
3	DTT	natural abundance	1 mM

2 2D 1H-13C HSQC aromatic

Instrument

Bruker Avance - 600 MHz

Sample

#	Name	Isotope labeling	Concentration
1	MAVS CARD	[U-99% 13C; U-99% 15N]	300 uM
2	sodium phosphate buffer	natural abundance	50 mM
3	DTT	natural abundance	1 mM

3 2D 1H-13C HSQC aliphatic

Instrument

Bruker Avance - 600 MHz

Sample

#	Name	Isotope labeling	Concentration
1	MAVS CARD	[U-99% 13C; U-99% 15N]	300 uM
2	sodium phosphate buffer	natural abundance	50 mM
3	DTT	natural abundance	1 mM

4 3D CBCA(CO)NH

Instrument

Bruker Avance - 600 MHz

Sample

#	Name	Isotope labeling	Concentration
1	MAVS CARD	[U-99% 13C; U-99% 15N]	300 uM
2	sodium phosphate buffer	natural abundance	50 mM
3	DTT	natural abundance	1 mM

5 3D HNCO

Instrument

Bruker Avance - 600 MHz

Sample

#	Name	Isotope labeling	Concentration
1	MAVS CARD	[U-99% 13C; U-99% 15N]	300 uM
2	sodium phosphate buffer	natural abundance	50 mM
3	DTT	natural abundance	1 mM

6 3D HN(CO)CA

Instrument

Bruker Avance - 600 MHz

Sample

#	Name	Isotope labeling	Concentration
1	MAVS CARD	[U-99% 13C; U-99% 15N]	300 uM
2	sodium phosphate buffer	natural abundance	50 mM
3	DTT	natural abundance	1 mM

7 3D HNCACB

Instrument

Bruker Avance - 600 MHz

Sample

#	Name	Isotope labeling	Concentration
1	MAVS CARD	[U-99% 13C; U-99% 15N]	300 uM
2	sodium phosphate buffer	natural abundance	50 mM
3	DTT	natural abundance	1 mM

8 3D 1H-15N TOCSY

Instrument

Bruker Avance - 600 MHz

Sample

#	Name	Isotope labeling	Concentration
1	MAVS CARD	[U-99% 13C; U-99% 15N]	300 uM
2	sodium phosphate buffer	natural abundance	50 mM
3	DTT	natural abundance	1 mM

9 3D 1H-15N NOESY

Instrument

Bruker Avance - 600 MHz

Sample

#	Name	Isotope labeling	Concentration
1	MAVS CARD	[U-99% 13C; U-99% 15N]	300 uM
2	sodium phosphate buffer	natural abundance	50 mM
3	DTT	natural abundance	1 mM

10 3D 1H-13C NOESY aliphatic

Instrument

Bruker Avance - 600 MHz

Sample

#	Name	Isotope labeling	Concentration
1	MAVS CARD	[U-99% 13C; U-99% 15N]	300 uM
2	sodium phosphate buffer	natural abundance	50 mM
3	DTT	natural abundance	1 mM

11 3D 1H-13C NOESY aromatic

Instrument

Bruker Avance - 600 MHz

Sample

#	Name	Isotope labeling	Concentration
1	MAVS CARD	[U-99% 13C; U-99% 15N]	300 uM
2	sodium phosphate buffer	natural abundance	50 mM
3	DTT	natural abundance	1 mM

12 3D HCCH-TOCSY

Instrument

Bruker Avance - 600 MHz

Sample

#	Name	Isotope labeling	Concentration
1	MAVS CARD	[U-99% 13C; U-99% 15N]	300 uM
2	sodium phosphate buffer	natural abundance	50 mM
3	DTT	natural abundance	1 mM

NMR combined restraints 4 contents Detail

Properties

Input source #1: NMR data (NEF) - Assigned chemical shifts, Distance restraints, Dihedral angle restraints	combined_25109_2ms8.nef
Input source #2: Coordindates	2ms8.cif
Diamagnetism of the molecular assembly	True (excluding Oxygen atoms)
Whether the assembly has a disulfide bond	None
Whether the assembly has a other bond	None
Whether the assembly contains a cyclic polymer	None
Overall data processing status	Warning

Disulfide bonds

None

Other bonds (neither disulfide, covalent nor hydrogen bonds, e.g. Zinc–sulphur bond)

None

Non-standard residues

None

Sequence alignments

Entity_assembly_ID (NMR): A vs Auth_asym_ID (model): A

-0--------10--------20--------30--------40--------50--------60--------70--------80--------90-------1
GSMPFAEDKTYKYICRNFSNFCNVDVVEILPYLPCLTARDQDRLRATCTLSGNRDTLWHLFNTLQRRPGWVEYFIAALRGCELVDLADEVASVYQSYQPR
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
GSMPFAEDKTYKYICRNFSNFCNVDVVEILPYLPCLTARDQDRLRATCTLSGNRDTLWHLFNTLQRRPGWVEYFIAALRGCELVDLADEVASVYQSYQPR
--------10--------20--------30--------40--------50--------60--------70--------80--------90-------100

00
TS
||
TS
--

Chain assignments

Entity_assembly_ID (NMR)	Auth_asym_ID (model)	Length	Unmapped	Conflict	Sequence coverage (%)
A	A	102	0	0	100.0

Content subtype: combined_25109_2ms8.nef

#	Content subtype	Saveframe	Status	# of rows (sets)	Experiment type	Sequence coverage (%)
1	Assigned chemical shifts	assigned_chem_shift_list_solution_NMR	Warning	1144		100.0 (chain: A, length: 102)
1	Distance restraints	CNS/XPLOR_distance_constraints_3	OK	1208 (1)	noe	99.0 (chain: A, length: 102)
1	Dihedral angle restraints	CNS/XPLOR_dihedral_2	OK	112 (112)	.	68.6 (chain: A, length: 102)

Assigned chemical shifts

Saveframe: assigned_chem_shift_list_solution_NMR
- Status: Warning
- Sequence coverage of experimental data
  - Entity_assembly_ID: A, Chain length: 102, Sequence coverage: 100.0%
- Total number of assignments
  - ¹H chemical shifts: 600
  - ¹³C chemical shifts: 428
  - ¹⁵N chemical shifts: 116
- Completeness of assignments
  - Entity_assemble_ID: A
- Redox state of CYS
- Peptide bond of PRO
- Tautomeric state of HIS
- Rotameric state of ILE , LEU, and VAL
- Histogram of normalized assigned chemical shifts
- Random coil index and derived parameters (S² and NMR RMSD)
  - Chain_ID: A

Atom group	# of target shifts	# of assigned shifts	Completeness (%)
¹H chemical shifts	620	599	96.6
¹³C chemical shifts	471	428	90.9
¹⁵N chemical shifts	117	116	99.1

Atom group	# of target shifts	# of assigned shifts	Completeness (%)
¹H chemical shifts	203	200	98.5
¹³C chemical shifts	204	178	87.3
¹⁵N chemical shifts	97	96	99.0

Atom group	# of target shifts	# of assigned shifts	Completeness (%)
¹H chemical shifts	417	399	95.7
¹³C chemical shifts	267	250	93.6
¹⁵N chemical shifts	20	20	100.0

Atom group	# of target shifts	# of assigned shifts	Completeness (%)
¹H chemical shifts	57	56	98.2
¹³C chemical shifts	57	56	98.2

Atom group	# of target shifts	# of assigned shifts	Completeness (%)
¹H chemical shifts	63	48	76.2
¹³C chemical shifts	61	46	75.4
¹⁵N chemical shifts	2	2	100.0

Distance restraints

Saveframe: CNS/XPLOR_distance_constraints_3
- Status: OK
- Experiment type: noe
- Sequence coverage of experimental data
  - Entity_assembly_ID: A, Chain length: 102, Sequence coverage: 99.0%
- Total number of restraints: 1207
- Weight of restraints: 1.0 (1207)
- Potential type of restraints: square-well-parabolic (1207)
- Range of distance target values: 2.28, 4.07 (Å)
- Histogram of distance target values
- Histogram of discrepancy in distance target values
- Distance restraints per residue
  - Chain_ID: A
- Distance restraints on contact map

Dihedral angle restraints

Saveframe: CNS/XPLOR_dihedral_2
- Status: OK
- Experiment type: .
- Sequence coverage of experimental data
  - Entity_assembly_ID: A, Chain length: 102, Sequence coverage: 68.6%
- Total number of restraints: 112
- Total number of restraints per polymer type: 112
- Weight of restraints: 1.0 (112)
- Potential type of restraints: square-well-parabolic (112)
- Plot of Phi-Psi angle restraints
- Dihedral angle restraints per residue
  - Chain_ID: A

Keywords MAVS CARD

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Link destinations for BMRB, PDB and EMDB

Found 1 Document (1.951 seconds)

BMRB: 25109

Sample

Chemical shift reference

Chemical shift reference

Chemical shift reference

Chemical shift reference

Related entities 1. MAVS CARD, : 1 : 1 : 6 : 9 entities Detail

Interaction partners 1. MAVS CARD, : 53 interactors Detail

Experiments performed 12 experiments Detail

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NMR combined restraints 4 contents Detail