BMRB: 25150

Solution structure of the human ubiquitin conjugating enzyme Ube2w

Authors

Vittal, V., Shi, L., Wenzel, D.M., Brzovic, P.S., Klevit, R.E.

Assembly

human ubiquitin conjugating enzyme Ube2w

Entity

1. human ubiquitin conjugating enzyme Ube2w (polymer), 151 monomers, 17330.67 Da Detail

MASMQKRLQK ELLALQNDPP PGMTLNEKSV QNSITQWIVD MEGAPGTLYE GEKFQLLFKF SSRYPFDSPQ VMFTGENIPV HPHVYSNGHI CLSILTEDWS PALSVQSVCL SIISMLSSCK EKRRPPDNSF YVRTCNKNPK KTKWWYHDDT C

Formula weight

17330.67 Da

Source organism

Homo sapiens

Exptl. method

solution NMR

Data set

assigned_chemical_shifts

Chem. Shift Complete

Sequence coverage: 91.4 %, Completeness: 43.8 %, Completeness (bb): 78.6 % Detail

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Polymer type: polypeptide(L)

	Total	1H	13C	15N
All	43.8 % (795 of 1816)	30.4 % (290 of 953)	55.3 % (389 of 704)	73.0 % (116 of 159)
Backbone	78.6 % (693 of 882)	71.3 % (211 of 296)	82.6 % (369 of 447)	81.3 % (113 of 139)
Sidechain	21.3 % (230 of 1079)	12.2 % (80 of 657)	36.6 % (147 of 402)	15.0 % (3 of 20)
Aromatic	0.0 % (0 of 164)	0.0 % (0 of 82)	0.0 % (0 of 78)	0.0 % (0 of 4)
Methyl	19.9 % (27 of 136)	19.1 % (13 of 68)	20.6 % (14 of 68)

1. entity

MASMQKRLQK ELLALQNDPP PGMTLNEKSV QNSITQWIVD MEGAPGTLYE GEKFQLLFKF SSRYPFDSPQ VMFTGENIPV HPHVYSNGHI CLSILTEDWS PALSVQSVCL SIISMLSSCK EKRRPPDNSF YVRTCNKNPK KTKWWYHDDT C

Show sample condition

Sample

Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 273 K, pH 7.0

#	Name	Isotope labeling	Type	Concentration
1	Ube2w	[U-100% 13C; U-100% 15N]		200.0 ~ 400.0 uM
2	H20	natural abundance		90 %
3	D20	natural abundance		10 %

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LACS Plot; CA

Referencing offset: -0.31 ppm, Outliers: 1 Detail

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Chemical shift reference

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
13C	DSS	methyl protons	0.0 ppm	na	indirect	0.2514495
1H	DSS	methyl protons	0.0 ppm	internal	direct	1.0
15N	DSS	methyl protons	0.0 ppm	na	indirect	0.1013291

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LACS Plot; CB

Referencing offset: -0.31 ppm, Outliers: 1 Detail

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Chemical shift reference

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
13C	DSS	methyl protons	0.0 ppm	na	indirect	0.2514495
1H	DSS	methyl protons	0.0 ppm	internal	direct	1.0
15N	DSS	methyl protons	0.0 ppm	na	indirect	0.1013291

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LACS Plot; HA

Referencing offset: -0.04 ppm, Outliers: 1 Detail

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Chemical shift reference

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
13C	DSS	methyl protons	0.0 ppm	na	indirect	0.2514495
1H	DSS	methyl protons	0.0 ppm	internal	direct	1.0
15N	DSS	methyl protons	0.0 ppm	na	indirect	0.1013291

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LACS Plot; CO

Referencing offset: 0.02 ppm, Outliers: 1 Detail

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Chemical shift reference

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
13C	DSS	methyl protons	0.0 ppm	na	indirect	0.2514495
1H	DSS	methyl protons	0.0 ppm	internal	direct	1.0
15N	DSS	methyl protons	0.0 ppm	na	indirect	0.1013291

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Protein Blocks Logo

Calculated from 20 models in PDB: 2MT6, Strand ID: A Detail

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Release date

2016-09-27

Citation

Intrinsic disorder drives N-terminal ubiquitination by Ube2w
Vittal, V., Shi, L., Wenzel, D.M., Scaglione, K., Duncan, E.D., Basrur, V., Elenitoba-Johnson, K.S.J., Baker, D., Paulson, H.L., Brzovic, P.S., Klevit, R.E.
Nat. Chem. Biol. (2015), 11, 83-89, PubMed 25436519 , DOI 10.1038/nchembio.1700 , Abstract

Related entities 1. human ubiquitin conjugating enzyme Ube2w, : 1 : 3 : 3 : 3 : 43 entities Detail

Interaction partners 1. human ubiquitin conjugating enzyme Ube2w, : 70 interactors Detail

Experiments performed 6 experiments Detail