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BMRB: 25153

2MT9

holo_FldB

Authors

Jin, C., Fu, W., Ye, Q.

Assembly

holo FldB

Entity

1. holo FldB, entity 1 (polymer), 173 monomers, 19699.95 Da Detail

MNMGLFYGSS TCYTEMAAEK IRDIIGPELV TLHNLKDDSP KLMEQYDVLI LGIPTWDFGE IQEDWEAVWD QLDDLNLEGK IVALYGLGDQ LGYGEWFLDA LGMLHDKLST KGVKFVGYWP TEGYEFTSPK PVIADGQLFV GLALDETNQY DLSDERIQSW CEQILNEMAE HYA

2. holo FldB, entity FMN (non-polymer), 456.344 Da

Total weight

20156.293 Da

Max. entity weight

19699.95 Da

Source organism

Escherichia coli

Exptl. method

solution NMR

Refine. method

simulated annealing

Data set

assigned_chemical_shifts

Chem. Shift Complete

Sequence coverage: 100.0 %, Completeness: 93.9 %, Completeness (bb): 99.7 % Detail

Polymer type: polypeptide(L)

	Total	¹H	¹³C	¹⁵N
All	93.9 % (1910 of 2034)	92.9 % (973 of 1047)	93.9 % (752 of 801)	99.5 % (185 of 186)
Backbone	99.7 % (1023 of 1026)	100.0 % (356 of 356)	99.4 % (500 of 503)	100.0 % (167 of 167)
Sidechain	89.6 % (1044 of 1165)	89.3 % (617 of 691)	89.9 % (409 of 455)	94.7 % (18 of 19)
Aromatic	63.9 % (138 of 216)	66.7 % (72 of 108)	58.8 % (60 of 102)	100.0 % (6 of 6)
Methyl	93.8 % (182 of 194)	93.8 % (91 of 97)	93.8 % (91 of 97)

1. entity 1

MNMGLFYGSS TCYTEMAAEK IRDIIGPELV TLHNLKDDSP KLMEQYDVLI LGIPTWDFGE IQEDWEAVWD QLDDLNLEGK IVALYGLGDQ LGYGEWFLDA LGMLHDKLST KGVKFVGYWP TEGYEFTSPK PVIADGQLFV GLALDETNQY DLSDERIQSW CEQILNEMAE HYA

Show sample condition

Sample #1

Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 298 K, pH 7

#	Name	Isotope labeling	Concentration
1	entity_1	[U-15N]	1 mM
2	sodium phosphate	natural abundance	30 mM
3	FLAVIN MONONUCLEOTIDE	natural abundance	2 mM

Sample #2

Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 298 K, pH 7

#	Name	Isotope labeling	Concentration
4	entity_1	[U-13C; U-15N]	1 mM
5	sodium phosphate	natural abundance	30 mM
6	FLAVIN MONONUCLEOTIDE	natural abundance	2 mM

LACS Plot; CA

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹³C	DSS	methyl protons	na	indirect	0.2514495
¹H	DSS	methyl protons	internal	direct	1.0
¹⁵N	DSS	methyl protons	na	indirect	0.1013291

Referencing offset: -0.03 ppm, Outliers: 1 Detail

LACS Plot; CB

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹³C	DSS	methyl protons	na	indirect	0.2514495
¹H	DSS	methyl protons	internal	direct	1.0
¹⁵N	DSS	methyl protons	na	indirect	0.1013291

Referencing offset: -0.03 ppm, Outliers: 1 Detail

LACS Plot; HA

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹³C	DSS	methyl protons	na	indirect	0.2514495
¹H	DSS	methyl protons	internal	direct	1.0
¹⁵N	DSS	methyl protons	na	indirect	0.1013291

Referencing offset: -0.08 ppm, Outliers: 1 Detail

LACS Plot; CO

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹³C	DSS	methyl protons	na	indirect	0.2514495
¹H	DSS	methyl protons	internal	direct	1.0
¹⁵N	DSS	methyl protons	na	indirect	0.1013291

Referencing offset: -0.13 ppm, Outliers: 1 Detail

Protein Blocks Logo

Calculated from 20 models in PDB: 2MT9, Strand ID: A Detail

Release date

2016-03-06

Citation

Long-chain flavodoxin FldB from Escherichia coli
Ye, Q., Fu, W., Hu, Y., Jin, C.
J. Biomol. NMR (2014), 60, 283-288, PubMed 25380767 , DOI 10.1007/s10858-014-9874-7 , Abstract

Related entities 1. holo FldB, entity 1, : 1 : 3 : 70 entities Detail

Experiments performed 8 experiments Detail

1 2D 1H-15N HSQC