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BMRB: 25155

2MTB

apo_FldB

Authors

Jin, C., Fu, W., Ye, Q.

Assembly

apo FldB

Entity

1. apo FldB (polymer), 173 monomers, 19699.95 Da Detail

MNMGLFYGSS TCYTEMAAEK IRDIIGPELV TLHNLKDDSP KLMEQYDVLI LGIPTWDFGE IQEDWEAVWD QLDDLNLEGK IVALYGLGDQ LGYGEWFLDA LGMLHDKLST KGVKFVGYWP TEGYEFTSPK PVIADGQLFV GLALDETNQY DLSDERIQSW CEQILNEMAE HYA

Formula weight

19699.95 Da

Source organism

Escherichia coli

Exptl. method

solution NMR

Refine. method

simulated annealing

Data set

assigned_chemical_shifts

Chem. Shift Complete

Sequence coverage: 95.4 %, Completeness: 79.2 %, Completeness (bb): 92.0 % Detail

Polymer type: polypeptide(L)

	Total	¹H	¹³C	¹⁵N
All	79.2 % (1611 of 2034)	77.9 % (816 of 1047)	79.0 % (633 of 801)	87.1 % (162 of 186)
Backbone	92.0 % (944 of 1026)	92.4 % (329 of 356)	92.6 % (466 of 503)	89.2 % (149 of 167)
Sidechain	69.8 % (813 of 1165)	70.5 % (487 of 691)	68.8 % (313 of 455)	68.4 % (13 of 19)
Aromatic	5.1 % (11 of 216)	8.3 % (9 of 108)	2.0 % (2 of 102)	0.0 % (0 of 6)
Methyl	86.1 % (167 of 194)	85.6 % (83 of 97)	86.6 % (84 of 97)

1. entity

MNMGLFYGSS TCYTEMAAEK IRDIIGPELV TLHNLKDDSP KLMEQYDVLI LGIPTWDFGE IQEDWEAVWD QLDDLNLEGK IVALYGLGDQ LGYGEWFLDA LGMLHDKLST KGVKFVGYWP TEGYEFTSPK PVIADGQLFV GLALDETNQY DLSDERIQSW CEQILNEMAE HYA

Show sample condition

Sample #1

Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 298 K, pH 7

#	Name	Isotope labeling	Type	Concentration
1	entity	[U-15N]		1 mM
2	sodium phosphate	natural abundance		30 mM

Sample #2

Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 298 K, pH 7

#	Name	Isotope labeling	Type	Concentration
3	entity	[U-13C; U-15N]		1 mM
4	sodium phosphate	natural abundance		30 mM

LACS Plot; CA

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹³C	DSS	methyl protons	na	indirect	0.2514495
¹H	DSS	methyl protons	internal	direct	1.0
¹⁵N	DSS	methyl protons	na	indirect	0.1013291

Referencing offset: -0.17 ppm, Outliers: 1 Detail

LACS Plot; CB

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹³C	DSS	methyl protons	na	indirect	0.2514495
¹H	DSS	methyl protons	internal	direct	1.0
¹⁵N	DSS	methyl protons	na	indirect	0.1013291

Referencing offset: -0.17 ppm, Outliers: 1 Detail

LACS Plot; HA

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹³C	DSS	methyl protons	na	indirect	0.2514495
¹H	DSS	methyl protons	internal	direct	1.0
¹⁵N	DSS	methyl protons	na	indirect	0.1013291

Referencing offset: -0.07 ppm, Outliers: 3 Detail

LACS Plot; CO

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹³C	DSS	methyl protons	na	indirect	0.2514495
¹H	DSS	methyl protons	internal	direct	1.0
¹⁵N	DSS	methyl protons	na	indirect	0.1013291

Referencing offset: -0.25 ppm, Outliers: 2 Detail

Protein Blocks Logo

Calculated from 20 models in PDB: 2MTB, Strand ID: A Detail

Release date

2016-03-06

Citation

Long-chain flavodoxin FldB from Escherichia coli
Ye, Q., Fu, W., Hu, Y., Jin, C.
J. Biomol. NMR (2014), 60, 283-288, PubMed 25380767 , DOI 10.1007/s10858-014-9874-7 , Abstract

Related entities 1. apo FldB, : 1 : 3 : 70 entities Detail

Experiments performed 9 experiments Detail

1 2D 1H-15N HSQC