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BMRB: 25156

2MTC

Structure of decorin binding protein A from strain N40 of Borrelia burgdorferi

Authors

Wang, X., Morgan, A.

Assembly

Decorin Binding Protein A

Entity

1. Decorin Binding Protein A (polymer, Thiol state: all disulfide bound), 166 monomers, 18263.63 Da Detail

GLKGETKIIL ERSAKDITDE INKIKKDAAD NNVNFAAFTD SETGSKVSEN SFILEAKVRA TTVAEKFVTA IEGEATKLKK TGSSGEFSAM YNMMLEVSGP LEELGVLRMT KTVTDAAEQH PTTTAEGILE IAKIMKTKLQ RVHTKNYCAL EKKKNPNFTD EKCKNN

Formula weight

18263.63 Da

Entity Connection

disulfide 1 Detail

ID	Type	Value order	Atom ID 1	Atom ID 2
1	disulfide	sing	1:CYS148:SG	1:CYS163:SG

Source organism

Borreliella burgdorferi N40

Exptl. method

solution NMR

Refine. method

simulated annealing

Data set

assigned_chemical_shifts

Chem. Shift Complete

Sequence coverage: 98.8 %, Completeness: 63.5 %, Completeness (bb): 82.4 % Detail

Polymer type: polypeptide(L)

	Total	¹H	¹³C	¹⁵N
All	63.5 % (1213 of 1911)	59.2 % (589 of 995)	63.0 % (466 of 740)	89.8 % (158 of 176)
Backbone	82.4 % (816 of 990)	94.4 % (319 of 338)	69.9 % (342 of 489)	95.1 % (155 of 163)
Sidechain	51.0 % (550 of 1078)	41.1 % (270 of 657)	67.9 % (277 of 408)	23.1 % (3 of 13)
Aromatic	11.9 % (10 of 84)	16.7 % (7 of 42)	7.1 % (3 of 42)
Methyl	80.9 % (152 of 188)	80.9 % (76 of 94)	80.9 % (76 of 94)

1. DBPA

GLKGETKIIL ERSAKDITDE INKIKKDAAD NNVNFAAFTD SETGSKVSEN SFILEAKVRA TTVAEKFVTA IEGEATKLKK TGSSGEFSAM YNMMLEVSGP LEELGVLRMT KTVTDAAEQH PTTTAEGILE IAKIMKTKLQ RVHTKNYCAL EKKKNPNFTD EKCKNN

Show sample condition

Sample

Solvent system 95% H2O/5% D2O, Pressure 1 atm, Temperature 298 K, pH 5.0

#	Name	Isotope labeling	Concentration
1	DBPA	[U-100% 13C; U-100% 15N]	0.8 mM
2	sodium phosphate	natural abundance	50 mM
3	sodium chloride	natural abundance	150 mM
4	H2O	natural abundance	95 %
5	D2O	natural abundance	5 %

LACS Plot; CA

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹³C	DSS	methyl protons	na	indirect	0.2514495
¹H	DSS	methyl protons	internal	direct	1.0
¹⁵N	DSS	methyl protons	na	indirect	0.1013291

Referencing offset: 0.31 ppm, Outliers: 1 Detail

LACS Plot; CB

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹³C	DSS	methyl protons	na	indirect	0.2514495
¹H	DSS	methyl protons	internal	direct	1.0
¹⁵N	DSS	methyl protons	na	indirect	0.1013291

Referencing offset: 0.31 ppm, Outliers: 1 Detail

LACS Plot; HA

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹³C	DSS	methyl protons	na	indirect	0.2514495
¹H	DSS	methyl protons	internal	direct	1.0
¹⁵N	DSS	methyl protons	na	indirect	0.1013291

Referencing offset: 0.1 ppm, Outliers: 1 Detail

Protein Blocks Logo

Calculated from 10 models in PDB: 2MTC, Strand ID: A Detail

Release date

2016-09-27

Citation

Structural mechanisms underlying sequence-dependent variations in GAG affinities of decorin binding protein A, a Borrelia burgdorferi adhesin
Wang, X., Morgan, A.
Biochem. J. (2015), 467, 439-451, PubMed 25695518 , DOI 10.1042/BJ20141201 , Abstract

Entries sharing articles BMRB: 1 entries Detail

BMRB: 25157 released on 2016-09-27
Title Strcucture of Decorin Binding Protein A from strain PBr of Borrelia garinii

Related entities 1. Decorin Binding Protein A, : 1 : 8 entities Detail

Experiments performed 9 experiments Detail

1 2D 1H-15N HSQC