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BMRB: 25157

2MTD

Strcucture of Decorin Binding Protein A from strain PBr of Borrelia garinii

Authors

Wang, X., Morgan, A.

Assembly

Decorin Binding Protein A

Entity

1. Decorin Binding Protein A (polymer, Thiol state: not present), 164 monomers, 17868.40 Da Detail

GLTGETKIRL ESSAQEIKDE INKIKANAKK EGVKFEAFTN TQTGSKISEK PEFILKAKIK AIQVAERFVK AIKEEAEKLK KSGSSGAFSA MYDLMIDVSK PLEEIGIQKM TGTVKEAAQK TPATTADGII AIAQAMEDKL NNVNKKQHDA LKNLKEKAKT ATTT

Formula weight

17868.4 Da

Source organism

Borreliella garinii PBr

Exptl. method

solution NMR

Refine. method

simulated annealing

Data set

assigned_chemical_shifts

Chem. Shift Complete

Sequence coverage: 100.0 %, Completeness: 87.4 %, Completeness (bb): 97.1 % Detail

Polymer type: polypeptide(L)

	Total	¹H	¹³C	¹⁵N
All	87.4 % (1681 of 1923)	81.7 % (825 of 1010)	94.0 % (694 of 738)	92.6 % (162 of 175)
Backbone	97.1 % (950 of 978)	96.7 % (323 of 334)	96.9 % (468 of 483)	98.8 % (159 of 161)
Sidechain	80.5 % (886 of 1100)	74.4 % (503 of 676)	92.7 % (380 of 410)	21.4 % (3 of 14)
Aromatic	58.1 % (36 of 62)	58.1 % (18 of 31)	58.1 % (18 of 31)
Methyl	100.0 % (190 of 190)	100.0 % (95 of 95)	100.0 % (95 of 95)

1. entity

GLTGETKIRL ESSAQEIKDE INKIKANAKK EGVKFEAFTN TQTGSKISEK PEFILKAKIK AIQVAERFVK AIKEEAEKLK KSGSSGAFSA MYDLMIDVSK PLEEIGIQKM TGTVKEAAQK TPATTADGII AIAQAMEDKL NNVNKKQHDA LKNLKEKAKT ATTT

Show sample condition

Sample

Solvent system 95% H2O/5% D2O, Pressure 1 atm, Temperature 298 K, pH 5.0

#	Name	Isotope labeling	Concentration
1	PBr	[U-100% 13C; U-100% 15N]	0.8 mM
2	sodium phosphate	natural abundance	50 mM
3	sodium chloride	natural abundance	150 mM
4	H2O	natural abundance	95 %
5	D2O	natural abundance	5 %

LACS Plot; CA

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹³C	DSS	methyl protons	na	indirect	0.2514495
¹H	DSS	methyl protons	internal	direct	1.0
¹⁵N	DSS	methyl protons	na	indirect	0.1013291

Referencing offset: 2.6 ppm, Outliers: 2 Detail

LACS Plot; CB

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹³C	DSS	methyl protons	na	indirect	0.2514495
¹H	DSS	methyl protons	internal	direct	1.0
¹⁵N	DSS	methyl protons	na	indirect	0.1013291

Referencing offset: 2.6 ppm, Outliers: 2 Detail

LACS Plot; HA

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹³C	DSS	methyl protons	na	indirect	0.2514495
¹H	DSS	methyl protons	internal	direct	1.0
¹⁵N	DSS	methyl protons	na	indirect	0.1013291

Referencing offset: 0.06 ppm, Outliers: 1 Detail

LACS Plot; CO

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹³C	DSS	methyl protons	na	indirect	0.2514495
¹H	DSS	methyl protons	internal	direct	1.0
¹⁵N	DSS	methyl protons	na	indirect	0.1013291

Referencing offset: -2.09 ppm, Outliers: 1 Detail

Protein Blocks Logo

Calculated from 10 models in PDB: 2MTD, Strand ID: A Detail

Release date

2016-09-27

Citation

Structural mechanisms underlying sequence-dependent variations in GAG affinities of decorin binding protein A, a Borrelia burgdorferi adhesin
Wang, X., Morgan, A.
Biochem. J. (2015), 467, 439-451, PubMed 25695518 , DOI 10.1042/BJ20141201 , Abstract

Related entities 1. Decorin Binding Protein A, : 1 : 9 entities Detail

Experiments performed 9 experiments Detail

1 2D 1H-15N HSQC