BMRBj - BMREntryMaster

	Total	¹H	¹³C	¹⁵N
All	83.0 % (1095 of 1320)	79.4 % (546 of 688)	85.0 % (438 of 515)	94.9 % (111 of 117)
Backbone	94.9 % (634 of 668)	93.1 % (216 of 232)	96.3 % (316 of 328)	94.4 % (102 of 108)
Sidechain	74.4 % (561 of 754)	72.4 % (330 of 456)	76.8 % (222 of 289)	100.0 % (9 of 9)
Aromatic	39.2 % (51 of 130)	44.6 % (29 of 65)	30.6 % (19 of 62)	100.0 % (3 of 3)
Methyl	86.5 % (83 of 96)	85.4 % (41 of 48)	87.5 % (42 of 48)

1. Aux-IAA17

GGPEAAAFVK VSMDGAPYLR KIDLRMYKSY DELSNALSNM FSSFTMGKHG GEEGMIDFMN ERKLMDLVNS WDYVPSYENK DGNWMLVGDV PWPMFVDTCK RLRLMKGSDA IGL

Show sample condition

Sample

Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 298 K, pH 7.4

#	Name	Isotope labeling	Concentration
1	Aux-IAA17	[U-99% 13C; U-99% 15N]	1 mM
2	sodium phosphate	natural abundance	10 mM
3	DTT	natural abundance	10 mM
4	H2O	natural abundance	90 %
5	D2O	natural abundance	10 %

LACS Plot; CA

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
¹H	water	protons	4.773 ppm	internal	direct	1.0
¹³C	water	protons	4.773 ppm	internal	indirect	0.2514495
¹⁵N	water	protons	4.773 ppm	internal	indirect	0.1013291

Referencing offset: -0.14 ppm, Outliers: 1 Detail

LACS Plot; CB

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
¹H	water	protons	4.773 ppm	internal	direct	1.0
¹³C	water	protons	4.773 ppm	internal	indirect	0.2514495
¹⁵N	water	protons	4.773 ppm	internal	indirect	0.1013291

Referencing offset: -0.14 ppm, Outliers: 1 Detail

LACS Plot; HA

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
¹H	water	protons	4.773 ppm	internal	direct	1.0
¹³C	water	protons	4.773 ppm	internal	indirect	0.2514495
¹⁵N	water	protons	4.773 ppm	internal	indirect	0.1013291

Referencing offset: -0.01 ppm, Outliers: 1 Detail

LACS Plot; CO

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
¹H	water	protons	4.773 ppm	internal	direct	1.0
¹³C	water	protons	4.773 ppm	internal	indirect	0.2514495
¹⁵N	water	protons	4.773 ppm	internal	indirect	0.1013291

Referencing offset: 0.19 ppm, Outliers: 1 Detail

Protein Blocks Logo

Calculated from 20 models in PDB: 2MUK, Strand ID: X Detail

Release date

2014-12-14

Citation

Structural basis for the auxin-induced transcriptional regulation by Aux/IAA17
Han, M., Park, Y., Kim, I., Kim, E., Yu, T., Rhee, S., Suh, J.
Proc. Natl. Acad. Sci. U. S. A. (2014), 111, 18613-18618, PubMed 25512488 , DOI 10.1073/pnas.1419525112 , Abstract

Related entities 1. AUX IAA17, : 1 : 2 : 1 : 200 entities Detail

Interaction partners 1. AUX IAA17, : 70 interactors Detail

More details for 70 interactors identified by 3 citations

Experiments performed 3 experiments Detail

NMR combined restraints 5 contents Detail

Properties

Input source #1: NMR data (NEF) - Assigned chemical shifts, Distance restraints, Dihedral angle restraints, RDC restraints	combined_25217_2muk.nef
Input source #2: Coordindates	2muk.cif
Diamagnetism of the molecular assembly	True (excluding Oxygen atoms)
Whether the assembly has a disulfide bond	None
Whether the assembly has a other bond	None
Whether the assembly contains a cyclic polymer	None
Overall data processing status	Warning

Disulfide bonds

None

Other bonds (neither disulfide, covalent nor hydrogen bonds, e.g. Zinc–sulphur bond)

None

Non-standard residues

None

Sequence alignments

Entity_assembly_ID (NMR): X vs Auth_asym_ID (model): X

Chain assignments

Entity_assembly_ID (NMR)	Auth_asym_ID (model)	Length	Unmapped	Conflict	Sequence coverage (%)
X	X	113	0	0	100.0

Content subtype: combined_25217_2muk.nef

#	Content subtype	Saveframe	Status	# of rows (sets)	Experiment type	Sequence coverage (%)
1	Assigned chemical shifts	assigned_chem_shift_list_1	Warning	1056		97.3 (chain: X, length: 113)
1	Distance restraints	CNS/XPLOR_distance_constraints_2	Warning	1861 (1)	noe	97.3 (chain: X, length: 113)
1	Dihedral angle restraints	CNS/XPLOR_dihedral_3	OK	172 (172)	.	94.7 (chain: X, length: 113)
1	RDC restraints	CNS/XPLOR_dipolar_coupling_4	OK	51 (51)	.	45.1 (chain: X, length: 113)

Assigned chemical shifts

Saveframe: assigned_chem_shift_list_1
- Status: Warning
- Sequence coverage of experimental data
  - Entity_assembly_ID: X, Chain length: 113, Sequence coverage: 97.3%
- Total number of assignments
  - ¹H chemical shifts: 520
  - ¹³C chemical shifts: 428
  - ¹⁵N chemical shifts: 108
- Completeness of assignments
  - Entity_assemble_ID: X
- Redox state of CYS
- Peptide bond of PRO
- Rotameric state of ILE , LEU, and VAL
- Histogram of normalized assigned chemical shifts
- Random coil index and derived parameters (S² and NMR RMSD)
  - Chain_ID: X

Atom group	# of target shifts	# of assigned shifts	Completeness (%)
¹H chemical shifts	688	520	75.6
¹³C chemical shifts	515	428	83.1
¹⁵N chemical shifts	122	108	88.5

Atom group	# of target shifts	# of assigned shifts	Completeness (%)
¹H chemical shifts	232	211	90.9
¹³C chemical shifts	226	216	95.6
¹⁵N chemical shifts	108	99	91.7

Atom group	# of target shifts	# of assigned shifts	Completeness (%)
¹H chemical shifts	456	309	67.8
¹³C chemical shifts	289	212	73.4
¹⁵N chemical shifts	14	9	64.3

Atom group	# of target shifts	# of assigned shifts	Completeness (%)
¹H chemical shifts	58	41	70.7
¹³C chemical shifts	58	41	70.7

Atom group	# of target shifts	# of assigned shifts	Completeness (%)
¹H chemical shifts	65	26	40.0
¹³C chemical shifts	62	16	25.8
¹⁵N chemical shifts	3	3	100.0

Distance restraints

Saveframe: CNS/XPLOR_distance_constraints_2
- Status: Warning
- Experiment type: noe
- Sequence coverage of experimental data
  - Entity_assembly_ID: X, Chain length: 113, Sequence coverage: 97.3%
- Total number of restraints: 1861
- Weight of restraints: 1.0 (1861)
- Potential type of restraints: square-well-parabolic (1861)
- Range of distance target values: 2.5, 4.15 (Å)
- Histogram of distance target values
- Histogram of discrepancy in distance target values
- Distance restraints per residue
  - Chain_ID: X
- Distance restraints on contact map

Dihedral angle restraints

Saveframe: CNS/XPLOR_dihedral_3
- Status: OK
- Experiment type: .
- Sequence coverage of experimental data
  - Entity_assembly_ID: X, Chain length: 113, Sequence coverage: 94.7%
- Total number of restraints: 172
- Total number of restraints per polymer type: 172
- Weight of restraints: 1.0 (172)
- Potential type of restraints: square-well-parabolic (172)
- Plot of Phi-Psi angle restraints
- Dihedral angle restraints per residue
  - Chain_ID: X

RDC restraints

Saveframe: CNS/XPLOR_dipolar_coupling_4
- Status: OK
- Experiment type: .
- Sequence coverage of experimental data
  - Entity_assembly_ID: X, Chain length: 113, Sequence coverage: 45.1%
- Total number of restraints: 51
- Potential type of restraints: undefined (51)
- Range of observed RDC values: 100.0, -100.0 (Hz)
- RDC restraints per residue

Keywords Aux/IAA

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Found 1 Document (1.1 seconds)

BMRB: 25217

Sample

Chemical shift reference

Chemical shift reference

Chemical shift reference

Chemical shift reference

Related entities 1. AUX IAA17, : 1 : 2 : 1 : 200 entities Detail

Interaction partners 1. AUX IAA17, : 70 interactors Detail

Experiments performed 3 experiments Detail

Instrument

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Instrument

Sample

Instrument

Sample

NMR combined restraints 5 contents Detail