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BMRB: 25230

2MUR

Solution Structure of the Human FAAP20 UBZ-Ubiquitin Complex

Authors

Wang, S., Wojtaszek, J.L., Zhou, P.

Assembly

Human FAAP20 UBZ-Ubiquitin Complex

Entity

1. UBZ (polymer, Thiol state: all other bound), 44 monomers, 4848.449 Da Detail

SHMGAAALRS CPMCQKEFAP RLTQLDVDSH LAQCLAESTE DVTW

2. Ubiquitin (polymer), 78 monomers, 8788.947 Da Detail

SHMQIFVKTL TGKTITLEVE PSDTIENVKA KIQDKEGIPP DQQRLIFAGK QLEDGRTLSD YNIQKESTLH LVLRLRGG

3. Human FAAP20 UBZ-Ubiquitin Complex, entity ZN (non-polymer), 65.409 Da

Total weight

13702.806 Da

Max. entity weight

8788.947 Da

Entity Connection

metal coordination 4 Detail

ID	Type	Value order	Atom ID 1	Atom ID 2
1	metal coordination	sing	1:CYS11:SG	3:ZN1:ZN
2	metal coordination	sing	1:CYS14:SG	3:ZN1:ZN
3	metal coordination	sing	1:CYS34:SG	3:ZN1:ZN
4	metal coordination	sing	1:HIS30:NE2	3:ZN1:ZN

Source organism

Homo sapiens

Exptl. method

solution NMR

Refine. method

torsion angle dynamics

Data set

assigned_chemical_shifts

Chem. Shift Complete

Sequence coverage: 99.2 %, Completeness: 96.1 %, Completeness (bb): 95.2 % Detail

Polymer type: polypeptide(L)

	Total	¹H	¹³C	¹⁵N
All	96.1 % (1355 of 1410)	96.3 % (708 of 735)	96.2 % (525 of 546)	94.6 % (122 of 129)
Backbone	95.2 % (687 of 722)	95.5 % (235 of 246)	95.3 % (342 of 359)	94.0 % (110 of 117)
Sidechain	97.1 % (780 of 803)	96.7 % (473 of 489)	97.7 % (295 of 302)	100.0 % (12 of 12)
Aromatic	87.9 % (58 of 66)	87.9 % (29 of 33)	87.5 % (28 of 32)	100.0 % (1 of 1)
Methyl	100.0 % (144 of 144)	100.0 % (72 of 72)	100.0 % (72 of 72)

1. UBZ

SHMGAAALRS CPMCQKEFAP RLTQLDVDSH LAQCLAESTE DVTW

2. Ubiquitin

SHMQIFVKTL TGKTITLEVE PSDTIENVKA KIQDKEGIPP DQQRLIFAGK QLEDGRTLSD YNIQKESTLH LVLRLRGG

Show sample condition

Sample #1

Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 298 K, pH 7

#	Name	Isotope labeling	Concentration
1	Ubiquitin	[U-100% 13C; U-100% 15N]	1 mM
2	sodium phosphate	natural abundance	25 mM
3	potassium chloride	natural abundance	100 mM
4	UBZ	natural abundance	2 mM

Sample #2

Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 298 K, pH 7

#	Name	Isotope labeling	Concentration
5	UBZ	[U-100% 13C; U-100% 15N]	0.8 mM
6	Ubiquitin	[U-100% 13C; U-100% 15N]	0.8 mM
7	sodium phosphate	natural abundance	25 mM
8	potassium chloride	natural abundance	100 mM

Sample #3

Solvent system 100% D2O, Pressure 1 atm, Temperature 298 K, pH 7

#	Name	Isotope labeling	Concentration
9	Ubiquitin	[U-100% 13C; U-100% 15N]	3 mM
10	sodium phosphate	natural abundance	25 mM
11	potassium chloride	natural abundance	100 mM
12	UBZ	[U-100% 13C; U-100% 15N]	3 mM

Sample #4

Solvent system 100% D2O, Pressure 1 atm, Temperature 298 K, pH 7

#	Name	Isotope labeling	Concentration
13	Ubiquitin	natural abundance	3 mM
14	sodium phosphate	natural abundance	25 mM
15	potassium chloride	natural abundance	100 mM
16	UBZ	[U-100% 13C; U-100% 15N]	3 mM

Sample #5

Solvent system 100% D2O, Pressure 1 atm, Temperature 298 K, pH 7

#	Name	Isotope labeling	Concentration
17	Ubiquitin	[U-100% 13C; U-100% 15N]	3 mM
18	sodium phosphate	natural abundance	25 mM
19	potassium chloride	natural abundance	100 mM
20	UBZ	natural abundance	3 mM

Sample #6

Solvent system 100% D2O, Pressure 1 atm, Temperature 298 K, pH 7

#	Name	Isotope labeling	Concentration
21	Ubiquitin	[U-100% 13C; U-100% 15N]	1 mM
22	sodium phosphate	natural abundance	25 mM
23	potassium chloride	natural abundance	100 mM
24	UBZ	natural abundance	2 mM

Sample #7

Solvent system 100% D2O, Pressure 1 atm, Temperature 298 K, pH 7

#	Name	Isotope labeling	Concentration
25	UBZ	[U-100% 13C; U-100% 15N]	0.8 mM
26	Ubiquitin	[U-100% 13C; U-100% 15N]	0.8 mM
27	sodium phosphate	natural abundance	25 mM
28	potassium chloride	natural abundance	100 mM

LACS Plot; CA

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹³C	DSS	methyl protons	na	indirect	0.2514495
¹H	DSS	methyl protons	internal	direct	1.0
¹⁵N	DSS	methyl protons	na	indirect	0.1013291

Referencing offset: 0.47 ppm, Outliers: 1 Detail

LACS Plot; CB

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹³C	DSS	methyl protons	na	indirect	0.2514495
¹H	DSS	methyl protons	internal	direct	1.0
¹⁵N	DSS	methyl protons	na	indirect	0.1013291

Referencing offset: 0.47 ppm, Outliers: 1 Detail

LACS Plot; HA

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹³C	DSS	methyl protons	na	indirect	0.2514495
¹H	DSS	methyl protons	internal	direct	1.0
¹⁵N	DSS	methyl protons	na	indirect	0.1013291

Referencing offset: -0.02 ppm, Outliers: 1 Detail

LACS Plot; CO

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹³C	DSS	methyl protons	na	indirect	0.2514495
¹H	DSS	methyl protons	internal	direct	1.0
¹⁵N	DSS	methyl protons	na	indirect	0.1013291

Referencing offset: 0.26 ppm, Outliers: 1 Detail

Protein Blocks Logo

Calculated from 10 models in PDB: 2MUR, Strand ID: A, B Detail

Release date

2016-09-21

Citation

Ubiquitin recognition by FAAP20 expands the complex interface beyond the canonical UBZ domain
Wojtaszek, J.L., Wang, S., Kim, H., Wu, Q., D'Andrea, A.D., Zhou, P.
Nucleic Acids Res. (2014), 42, 13997-14005, PubMed 25414354 , DOI 10.1093/nar/gku1153 , Abstract

Entries sharing articles BMRB: 1 entries Detail

BMRB: 25229 released on 2016-09-22
Title Solution Structure of the Human FAAP20 UBZ

Related entities 1. UBZ, : 1 : 1 : 2 : 10 entities Detail

Related entities 2. Ubiquitin, : 138 : 188 entities Detail

Interaction partners 1. UBZ, : 9 interactors Detail

More details for 9 interactors identified by 4 citations

Experiments performed 27 experiments Detail

1 2D 1H-15N HSQC

Instrument

Varian INOVA - 600 MHz

Sample

State isotropic, Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 298 K, pH 7

#	Name	Isotope labeling	Concentration
1	Ubiquitin	[U-100% 13C; U-100% 15N]	1 mM
2	sodium phosphate	natural abundance	25 mM
3	potassium chloride	natural abundance	100 mM
4	UBZ	natural abundance	2 mM

2 2D 1H-15N HSQC

Instrument

Varian INOVA - 600 MHz

Sample

State isotropic, Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 298 K, pH 7

#	Name	Isotope labeling	Concentration
5	UBZ	[U-100% 13C; U-100% 15N]	0.8 mM
6	Ubiquitin	[U-100% 13C; U-100% 15N]	0.8 mM
7	sodium phosphate	natural abundance	25 mM
8	potassium chloride	natural abundance	100 mM

3 4D sparse-sampled HCCH TOCSY

Instrument

Varian INOVA - 600 MHz

Sample

State isotropic, Solvent system 100% D2O, Pressure 1 atm, Temperature 298 K, pH 7

#	Name	Isotope labeling	Concentration
21	Ubiquitin	[U-100% 13C; U-100% 15N]	1 mM
22	sodium phosphate	natural abundance	25 mM
23	potassium chloride	natural abundance	100 mM
24	UBZ	natural abundance	2 mM

4 3D sparse-sampled HA(CA)NH

Instrument

Varian INOVA - 600 MHz

Sample

State isotropic, Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 298 K, pH 7

#	Name	Isotope labeling	Concentration
1	Ubiquitin	[U-100% 13C; U-100% 15N]	1 mM
2	sodium phosphate	natural abundance	25 mM
3	potassium chloride	natural abundance	100 mM
4	UBZ	natural abundance	2 mM

5 3D sparse-sampled HNCO

Instrument

Varian INOVA - 600 MHz

Sample

State isotropic, Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 298 K, pH 7

#	Name	Isotope labeling	Concentration
1	Ubiquitin	[U-100% 13C; U-100% 15N]	1 mM
2	sodium phosphate	natural abundance	25 mM
3	potassium chloride	natural abundance	100 mM
4	UBZ	natural abundance	2 mM

6 3D sparse-sampled HNCA

Instrument

Varian INOVA - 600 MHz

Sample

State isotropic, Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 298 K, pH 7

#	Name	Isotope labeling	Concentration
1	Ubiquitin	[U-100% 13C; U-100% 15N]	1 mM
2	sodium phosphate	natural abundance	25 mM
3	potassium chloride	natural abundance	100 mM
4	UBZ	natural abundance	2 mM

7 3D sparse-sampled HNCACB

Instrument

Varian INOVA - 600 MHz

Sample

State isotropic, Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 298 K, pH 7

#	Name	Isotope labeling	Concentration
1	Ubiquitin	[U-100% 13C; U-100% 15N]	1 mM
2	sodium phosphate	natural abundance	25 mM
3	potassium chloride	natural abundance	100 mM
4	UBZ	natural abundance	2 mM

8 3D sparse-sampled HN(CO)CA

Instrument

Varian INOVA - 600 MHz

Sample

State isotropic, Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 298 K, pH 7

#	Name	Isotope labeling	Concentration
1	Ubiquitin	[U-100% 13C; U-100% 15N]	1 mM
2	sodium phosphate	natural abundance	25 mM
3	potassium chloride	natural abundance	100 mM
4	UBZ	natural abundance	2 mM

9 3D sparse-sampled HN(COCA)CB

Instrument

Varian INOVA - 600 MHz

Sample

State isotropic, Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 298 K, pH 7

#	Name	Isotope labeling	Concentration
1	Ubiquitin	[U-100% 13C; U-100% 15N]	1 mM
2	sodium phosphate	natural abundance	25 mM
3	potassium chloride	natural abundance	100 mM
4	UBZ	natural abundance	2 mM

10 4D sparse-sampled HC(CO)NH-TOCSY

Instrument

Varian INOVA - 600 MHz

Sample

State isotropic, Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 298 K, pH 7

#	Name	Isotope labeling	Concentration
1	Ubiquitin	[U-100% 13C; U-100% 15N]	1 mM
2	sodium phosphate	natural abundance	25 mM
3	potassium chloride	natural abundance	100 mM
4	UBZ	natural abundance	2 mM

11 4D sparse-sampled CHNH NOESY

Instrument

Varian INOVA - 600 MHz

Sample

State isotropic, Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 298 K, pH 7

#	Name	Isotope labeling	Concentration
1	Ubiquitin	[U-100% 13C; U-100% 15N]	1 mM
2	sodium phosphate	natural abundance	25 mM
3	potassium chloride	natural abundance	100 mM
4	UBZ	natural abundance	2 mM

12 3D 1H-15N NOESY

Instrument

Varian INOVA - 600 MHz

Sample

State isotropic, Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 298 K, pH 7

#	Name	Isotope labeling	Concentration
1	Ubiquitin	[U-100% 13C; U-100% 15N]	1 mM
2	sodium phosphate	natural abundance	25 mM
3	potassium chloride	natural abundance	100 mM
4	UBZ	natural abundance	2 mM

13 3D sparse-sampled HA(CACO)NH

Instrument

Varian INOVA - 600 MHz

Sample

State isotropic, Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 298 K, pH 7

#	Name	Isotope labeling	Concentration
5	UBZ	[U-100% 13C; U-100% 15N]	0.8 mM
6	Ubiquitin	[U-100% 13C; U-100% 15N]	0.8 mM
7	sodium phosphate	natural abundance	25 mM
8	potassium chloride	natural abundance	100 mM

14 3D sparse-sampled HA(CA)NH

Instrument

Varian INOVA - 600 MHz

Sample

State isotropic, Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 298 K, pH 7

#	Name	Isotope labeling	Concentration
5	UBZ	[U-100% 13C; U-100% 15N]	0.8 mM
6	Ubiquitin	[U-100% 13C; U-100% 15N]	0.8 mM
7	sodium phosphate	natural abundance	25 mM
8	potassium chloride	natural abundance	100 mM

15 3D sparse-sampled HNCO

Instrument

Varian INOVA - 600 MHz

Sample

State isotropic, Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 298 K, pH 7

#	Name	Isotope labeling	Concentration
5	UBZ	[U-100% 13C; U-100% 15N]	0.8 mM
6	Ubiquitin	[U-100% 13C; U-100% 15N]	0.8 mM
7	sodium phosphate	natural abundance	25 mM
8	potassium chloride	natural abundance	100 mM

16 3D sparse-sampled HNCA

Instrument

Varian INOVA - 600 MHz

Sample

State isotropic, Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 298 K, pH 7

#	Name	Isotope labeling	Concentration
5	UBZ	[U-100% 13C; U-100% 15N]	0.8 mM
6	Ubiquitin	[U-100% 13C; U-100% 15N]	0.8 mM
7	sodium phosphate	natural abundance	25 mM
8	potassium chloride	natural abundance	100 mM

17 3D sparse-sampled HNCACB

Instrument

Varian INOVA - 600 MHz

Sample

State isotropic, Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 298 K, pH 7

#	Name	Isotope labeling	Concentration
5	UBZ	[U-100% 13C; U-100% 15N]	0.8 mM
6	Ubiquitin	[U-100% 13C; U-100% 15N]	0.8 mM
7	sodium phosphate	natural abundance	25 mM
8	potassium chloride	natural abundance	100 mM

18 3D sparse-sampled HN(CO)CA

Instrument

Varian INOVA - 600 MHz

Sample

State isotropic, Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 298 K, pH 7

#	Name	Isotope labeling	Concentration
5	UBZ	[U-100% 13C; U-100% 15N]	0.8 mM
6	Ubiquitin	[U-100% 13C; U-100% 15N]	0.8 mM
7	sodium phosphate	natural abundance	25 mM
8	potassium chloride	natural abundance	100 mM

19 4D sparse-sampled HC(CO)NH-TOCSY

Instrument

Varian INOVA - 600 MHz

Sample

State isotropic, Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 298 K, pH 7

#	Name	Isotope labeling	Concentration
5	UBZ	[U-100% 13C; U-100% 15N]	0.8 mM
6	Ubiquitin	[U-100% 13C; U-100% 15N]	0.8 mM
7	sodium phosphate	natural abundance	25 mM
8	potassium chloride	natural abundance	100 mM

20 4D sparse-sampled CHNH NOESY

Instrument

Varian INOVA - 600 MHz

Sample

State isotropic, Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 298 K, pH 7

#	Name	Isotope labeling	Concentration
5	UBZ	[U-100% 13C; U-100% 15N]	0.8 mM
6	Ubiquitin	[U-100% 13C; U-100% 15N]	0.8 mM
7	sodium phosphate	natural abundance	25 mM
8	potassium chloride	natural abundance	100 mM

21 3D sparse-sampled HN(COCA)CB

Instrument

Varian INOVA - 600 MHz

Sample

State isotropic, Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 298 K, pH 7

#	Name	Isotope labeling	Concentration
5	UBZ	[U-100% 13C; U-100% 15N]	0.8 mM
6	Ubiquitin	[U-100% 13C; U-100% 15N]	0.8 mM
7	sodium phosphate	natural abundance	25 mM
8	potassium chloride	natural abundance	100 mM

22 3D 1H-15N NOESY

Instrument

Varian INOVA - 600 MHz

Sample

State isotropic, Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 298 K, pH 7

#	Name	Isotope labeling	Concentration
5	UBZ	[U-100% 13C; U-100% 15N]	0.8 mM
6	Ubiquitin	[U-100% 13C; U-100% 15N]	0.8 mM
7	sodium phosphate	natural abundance	25 mM
8	potassium chloride	natural abundance	100 mM

23 4D sparse-sampled CHCH NOESY

Instrument

Varian INOVA - 600 MHz

Sample

State isotropic, Solvent system 100% D2O, Pressure 1 atm, Temperature 298 K, pH 7

#	Name	Isotope labeling	Concentration
9	Ubiquitin	[U-100% 13C; U-100% 15N]	3 mM
10	sodium phosphate	natural abundance	25 mM
11	potassium chloride	natural abundance	100 mM
12	UBZ	[U-100% 13C; U-100% 15N]	3 mM

24 4D sparse-sampled CHCH NOESY

Instrument

Varian INOVA - 600 MHz

Sample

State isotropic, Solvent system 100% D2O, Pressure 1 atm, Temperature 298 K, pH 7

#	Name	Isotope labeling	Concentration
13	Ubiquitin	natural abundance	3 mM
14	sodium phosphate	natural abundance	25 mM
15	potassium chloride	natural abundance	100 mM
16	UBZ	[U-100% 13C; U-100% 15N]	3 mM

25 4D sparse-sampled CHCH NOESY

Instrument

Varian INOVA - 600 MHz

Sample

State isotropic, Solvent system 100% D2O, Pressure 1 atm, Temperature 298 K, pH 7

#	Name	Isotope labeling	Concentration
17	Ubiquitin	[U-100% 13C; U-100% 15N]	3 mM
18	sodium phosphate	natural abundance	25 mM
19	potassium chloride	natural abundance	100 mM
20	UBZ	natural abundance	3 mM

26 3D sparse-sampled HNCACO

Instrument

Varian INOVA - 600 MHz

Sample

State isotropic, Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 298 K, pH 7

#	Name	Isotope labeling	Concentration
5	UBZ	[U-100% 13C; U-100% 15N]	0.8 mM
6	Ubiquitin	[U-100% 13C; U-100% 15N]	0.8 mM
7	sodium phosphate	natural abundance	25 mM
8	potassium chloride	natural abundance	100 mM

27 4D sparse-sampled HCCH TOCSY

Instrument

Varian INOVA - 600 MHz

Sample

State isotropic, Solvent system 100% D2O, Pressure 1 atm, Temperature 298 K, pH 7

#	Name	Isotope labeling	Concentration
25	UBZ	[U-100% 13C; U-100% 15N]	0.8 mM
26	Ubiquitin	[U-100% 13C; U-100% 15N]	0.8 mM
27	sodium phosphate	natural abundance	25 mM
28	potassium chloride	natural abundance	100 mM

NMR combined restraints 5 contents Detail

Properties

Input source #1: NMR data (NEF) - Assigned chemical shifts, Distance restraints, Dihedral angle restraints	combined_25230_2mur.nef
Input source #2: Coordindates	2mur.cif
Diamagnetism of the molecular assembly	True (excluding Oxygen atoms)
Whether the assembly has a disulfide bond	None
Whether the assembly has a other bond	True (see coodinates for details)
Whether the assembly contains a cyclic polymer	None
Overall data processing status	Warning

Disulfide bonds

None

Other bonds (neither disulfide, covalent nor hydrogen bonds, e.g. Zinc–sulphur bond)

Ptnr_site_1	Ptnr_site_2	Redox_state_prediction_1	Redox_state_prediction_2	Distance (Å)
1:30:HIS:NE2	3:1:ZN:ZN	unknown	unknown	n/a
1:34:CYS:SG	3:1:ZN:ZN	unknown	unknown	n/a
1:14:CYS:SG	3:1:ZN:ZN	unknown	unknown	n/a
1:11:CYS:SG	3:1:ZN:ZN	unknown	unknown	n/a

Non-standard residues

Chain_ID	Seq_ID	Comp_ID	Chem_comp_name	Experimental evidences
C	1	ZN	ZINC ION	None

Sequence alignments

Entity_assembly_ID (NMR): A vs Auth_asym_ID (model): A

--------10--------20--------30--------40----
SHMGAAALRSCPMCQKEFAPRLTQLDVDSHLAQCLAESTEDVTW
||||||||||||||||||||||||||||||||||||||||||||
SHMGAAALRSCPMCQKEFAPRLTQLDVDSHLAQCLAESTEDVTW

Entity_assembly_ID (NMR): B vs Auth_asym_ID (model): B

-100-----110-------120-------130-------140-------150-------160-------170------
SHMQIFVKTLTGKTITLEVEPSDTIENVKAKIQDKEGIPPDQQRLIFAGKQLEDGRTLSDYNIQKESTLHLVLRLRGG
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
SHMQIFVKTLTGKTITLEVEPSDTIENVKAKIQDKEGIPPDQQRLIFAGKQLEDGRTLSDYNIQKESTLHLVLRLRGG
--------10--------20--------30--------40--------50--------60--------70--------

Chain assignments

Entity_assembly_ID (NMR)	Auth_asym_ID (model)	Length	Unmapped	Conflict	Sequence coverage (%)
A	A	44	0	0	100.0
B	B	78	0	0	100.0

Content subtype: combined_25230_2mur.nef

#	Content subtype	Saveframe	Status	# of rows (sets)	Experiment type	Sequence coverage (%)
1	Covalent bonds	assembly	OK	4		No information
1	Assigned chemical shifts	assigned_chem_shift_list_1	Warning	1354		97.7 (chain: A, length: 44), 100.0 (chain: B, length: 78)
1	Distance restraints	DYANA/DIANA_distance_constraints_2	OK	5659 (1)	noe	95.5 (chain: A, length: 44), 98.7 (chain: B, length: 78)
2	Distance restraints	DYANA/DIANA_distance_constraints_3	Warning	93 (1)	hbond	50.0 (chain: A, length: 44), 53.8 (chain: B, length: 78)
1	Dihedral angle restraints	DYANA/DIANA_dihedral_4	OK	186 (186)	.	79.5 (chain: A, length: 44), 94.9 (chain: B, length: 78)

Assigned chemical shifts

Saveframe: assigned_chem_shift_list_1
- Status: Warning
- Sequence coverage of experimental data
  - Entity_assembly_ID: A, Chain length: 44, Sequence coverage: 97.7%
  - Entity_assembly_ID: B, Chain length: 78, Sequence coverage: 100.0%
- Total number of assignments
  - ¹³C chemical shifts: 522
  - ¹H chemical shifts: 710
  - ¹⁵N chemical shifts: 122
- Completeness of assignments
  - Entity_assemble_ID: A
  - Entity_assemble_ID: B
- Redox state of CYS
- Peptide bond of PRO
- Tautomeric state of HIS
- Rotameric state of ILE , LEU, and VAL
- Histogram of normalized assigned chemical shifts
- Random coil index and derived parameters (S² and NMR RMSD)
  - Chain_ID: A
  - Chain_ID: B

Atom group	# of target shifts	# of assigned shifts	Completeness (%)
¹³C chemical shifts	188	176	93.6
¹H chemical shifts	247	234	94.7
¹⁵N chemical shifts	48	44	91.7

Atom group	# of target shifts	# of assigned shifts	Completeness (%)
¹³C chemical shifts	88	80	90.9
¹H chemical shifts	87	82	94.3
¹⁵N chemical shifts	42	40	95.2

Atom group	# of target shifts	# of assigned shifts	Completeness (%)
¹³C chemical shifts	100	96	96.0
¹H chemical shifts	160	152	95.0
¹⁵N chemical shifts	6	4	66.7

Atom group	# of target shifts	# of assigned shifts	Completeness (%)
¹³C chemical shifts	25	25	100.0
¹H chemical shifts	25	25	100.0

Atom group	# of target shifts	# of assigned shifts	Completeness (%)
¹³C chemical shifts	14	12	85.7
¹H chemical shifts	15	13	86.7
¹⁵N chemical shifts	1	1	100.0

Covalent bonds

Saveframe: assembly
- Status: OK

Distance restraints

Saveframe: DYANA/DIANA_distance_constraints_2
- Status: OK
- Experiment type: noe
- Sequence coverage of experimental data
  - Entity_assembly_ID: A, Chain length: 44, Sequence coverage: 95.5%
  - Entity_assembly_ID: B, Chain length: 78, Sequence coverage: 98.7%
- Total number of restraints: 5637
- Weight of restraints: 1.0 (5637)
- Potential type of restraints: upper-bound-parabolic (5637)
- Range of distance target values: 2.42, 7.0 (Å)
- Histogram of distance target values
- Histogram of discrepancy in distance target values
- Distance restraints per residue
  - Chain_ID: A
  - Chain_ID: B
  - Chain_ID: C
    None
- Distance restraints on contact map
- Saveframe: DYANA/DIANA_distance_constraints_3
  - Status: Warning
  - Experiment type: hbond
  - Sequence coverage of experimental data
    - Entity_assembly_ID: A, Chain length: 44, Sequence coverage: 50.0%
    - Entity_assembly_ID: B, Chain length: 78, Sequence coverage: 53.8%
  - Total number of restraints: 93
  - Weight of restraints: 1.0 (93)
  - Potential type of restraints: upper-bound-parabolic (93)
  - Range of distance target values: 2.5, 4.0 (Å)
  - Histogram of distance target values
  - Distance restraints per residue
    - Chain_ID: A
    - Chain_ID: B
    - Chain_ID: C
      None
  - Distance restraints on contact map

Dihedral angle restraints

Saveframe: DYANA/DIANA_dihedral_4
- Status: OK
- Experiment type: .
- Sequence coverage of experimental data
  - Entity_assembly_ID: A, Chain length: 44, Sequence coverage: 79.5%
  - Entity_assembly_ID: B, Chain length: 78, Sequence coverage: 94.9%
- Total number of restraints: 186
- Total number of restraints per polymer type: 186
- Weight of restraints: 1.0 (186)
- Potential type of restraints: square-well-parabolic (186)
- Plot of Phi-Psi angle restraints
- Plot of Chi1-Chi2 angle restraints
- Dihedral angle restraints per residue
  - Chain_ID: A
  - Chain_ID: B

Keywords FAAP20, Fanconi Anemia, UBZ, ubiquitin, zinc-finger

Atom group	# of target shifts	# of assigned shifts	Completeness (%)
¹³C chemical shifts	358	346	96.6
¹H chemical shifts	488	476	97.5
¹⁵N chemical shifts	87	78	89.7

Atom group	# of target shifts	# of assigned shifts	Completeness (%)
¹³C chemical shifts	156	147	94.2
¹H chemical shifts	159	155	97.5
¹⁵N chemical shifts	75	70	93.3

Atom group	# of target shifts	# of assigned shifts	Completeness (%)
¹³C chemical shifts	202	199	98.5
¹H chemical shifts	329	321	97.6
¹⁵N chemical shifts	12	8	66.7

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Found 1 Document (0.958 seconds)

BMRB: 25230

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Chemical shift reference

Chemical shift reference

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Chemical shift reference

Entries sharing articles BMRB: 1 entries Detail

Related entities 1. UBZ, : 1 : 1 : 2 : 10 entities Detail

Related entities 2. Ubiquitin, : 138 : 188 entities Detail

Interaction partners 1. UBZ, : 9 interactors Detail

Experiments performed 27 experiments Detail

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