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Found 1 Document (1.249 seconds)

BMRB: 25237

2N45

Solution NMR Structure of Maltose-binding protein from Escherichia coli, Northeast Structural Genomics Consortium (NESG) Target ER690

Authors

Rossi, P., Lange, O.F., Sgourakis, N.G., Song, Y., Lee, H., Aramini, J.M., Ertekin, A., Xiao, R., Acton, T.B., Baker, D., Montelione, G.T.

Assembly

MBP

Entity

1. MBP (polymer, Thiol state: not present), 370 monomers, 40676.74 Da Detail

KIEEGKLVIW INGDKGYNGL AEVGKKFEKD TGIKVTVEHP DKLEEKFPQV AATGDGPDII FWAHDRFGGY AQSGLLAEIT PDKAFQDKLY PFTWDAVRYN GKLIAYPIAV EALSLIYNKD LLPNPPKTWE EIPALDKELK AKGKSALMFN LQEPYFTWPL IAADGGYAFK YENGKYDIKD VGVDNAGAKA GLTFLVDLIK NKHMNADTDY SIAEAAFNKG ETAMTINGPR AWSNIDTSKV NYGVTVLPTF KGQPSKPFVG VLSAGINAAS PNKELAKEFL ENYLLTDEGL EAVNKDKPLG AVALKSYEEE LAKDPRIAAT MENAQKGEIM PNIPQMSAFW YAVRTAVINA ASGRQTVDEA LKDAQTRITK

Formula weight

40676.74 Da

Source organism

Escherichia coli

Exptl. method

Refine. method

simulated annealing, molecular dynamics

Data set

assigned_chemical_shifts

Chem. Shift Complete

Sequence coverage: 98.1 %, Completeness: 55.6 %, Completeness (bb): 82.6 % Detail

Polymer type: polypeptide(L)

	Total	¹H	¹³C	¹⁵N
All	55.6 % (2399 of 4316)	36.8 % (825 of 2239)	72.5 % (1226 of 1691)	90.2 % (348 of 386)
Backbone	82.6 % (1800 of 2178)	58.3 % (436 of 748)	95.0 % (1027 of 1081)	96.6 % (337 of 349)
Sidechain	37.6 % (932 of 2479)	26.2 % (390 of 1491)	55.8 % (531 of 951)	29.7 % (11 of 37)
Aromatic	14.2 % (52 of 366)	17.5 % (32 of 183)	7.4 % (13 of 176)	100.0 % (7 of 7)
Methyl	76.0 % (319 of 420)	68.1 % (143 of 210)	83.8 % (176 of 210)

1. ER690

KIEEGKLVIW INGDKGYNGL AEVGKKFEKD TGIKVTVEHP DKLEEKFPQV AATGDGPDII FWAHDRFGGY AQSGLLAEIT PDKAFQDKLY PFTWDAVRYN GKLIAYPIAV EALSLIYNKD LLPNPPKTWE EIPALDKELK AKGKSALMFN LQEPYFTWPL IAADGGYAFK YENGKYDIKD VGVDNAGAKA GLTFLVDLIK NKHMNADTDY SIAEAAFNKG ETAMTINGPR AWSNIDTSKV NYGVTVLPTF KGQPSKPFVG VLSAGINAAS PNKELAKEFL ENYLLTDEGL EAVNKDKPLG AVALKSYEEE LAKDPRIAAT MENAQKGEIM PNIPQMSAFW YAVRTAVINA ASGRQTVDEA LKDAQTRITK

Show sample condition

Sample

Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 310 K, pH 7.2, Details 1.05 mM ER690.005, 90% H2O/10% D2O

#	Name	Isotope labeling	Type	Concentration
1	ER690	natural abundance		1.05 mM
2	H20	natural abundance		90 %
3	D2O	natural abundance		10 %

Protein Blocks Logo

Calculated from 10 models in PDB: 2MV0, Strand ID: A Detail

Release date

2014-12-07

Related entities 1. MBP, : 1 : 3 : 98 : 12 entities Detail

Interaction partners 1. MBP, : 1 interactors Detail

More details for 1 interactors identified by 1 citations

Experiments performed 13 experiments Detail

1 2D 1H-15N HSQC

Instrument

Bruker Avance - 800 MHz

Sample

State isotropic, Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 310 K, pH 7.2, Details 1.05 mM ER690.005, 90% H2O/10% D2O

#	Name	Isotope labeling	Type	Concentration
1	ER690	natural abundance		1.05 mM
2	H20	natural abundance		90 %
3	D2O	natural abundance		10 %

2 2D 1H-13C HSQC

Instrument

Bruker Avance - 800 MHz

Sample

State isotropic, Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 310 K, pH 7.2, Details 1.05 mM ER690.005, 90% H2O/10% D2O

#	Name	Isotope labeling	Type	Concentration
1	ER690	natural abundance		1.05 mM
2	H20	natural abundance		90 %
3	D2O	natural abundance		10 %

3 3D HNCO

Instrument

Bruker Avance - 800 MHz

Sample

State isotropic, Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 310 K, pH 7.2, Details 1.05 mM ER690.005, 90% H2O/10% D2O

#	Name	Isotope labeling	Type	Concentration
1	ER690	natural abundance		1.05 mM
2	H20	natural abundance		90 %
3	D2O	natural abundance		10 %

4 3D CBCA(CO)NH

Instrument

Bruker Avance - 800 MHz

Sample

State isotropic, Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 310 K, pH 7.2, Details 1.05 mM ER690.005, 90% H2O/10% D2O

#	Name	Isotope labeling	Type	Concentration
1	ER690	natural abundance		1.05 mM
2	H20	natural abundance		90 %
3	D2O	natural abundance		10 %

5 3D HNCACB

Instrument

Bruker Avance - 800 MHz

Sample

State isotropic, Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 310 K, pH 7.2, Details 1.05 mM ER690.005, 90% H2O/10% D2O

#	Name	Isotope labeling	Type	Concentration
1	ER690	natural abundance		1.05 mM
2	H20	natural abundance		90 %
3	D2O	natural abundance		10 %

6 3D 1H-13C NOESY

Instrument

Bruker Avance - 800 MHz

Sample

State isotropic, Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 310 K, pH 7.2, Details 1.05 mM ER690.005, 90% H2O/10% D2O

#	Name	Isotope labeling	Type	Concentration
1	ER690	natural abundance		1.05 mM
2	H20	natural abundance		90 %
3	D2O	natural abundance		10 %

7 3D 1H-15N NOESY

Instrument

Bruker Avance - 800 MHz

Sample

State isotropic, Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 310 K, pH 7.2, Details 1.05 mM ER690.005, 90% H2O/10% D2O

#	Name	Isotope labeling	Type	Concentration
1	ER690	natural abundance		1.05 mM
2	H20	natural abundance		90 %
3	D2O	natural abundance		10 %

8 3D (h)CCH-TOCSY

Instrument

Bruker Avance - 800 MHz

Sample

State isotropic, Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 310 K, pH 7.2, Details 1.05 mM ER690.005, 90% H2O/10% D2O

#	Name	Isotope labeling	Type	Concentration
1	ER690	natural abundance		1.05 mM
2	H20	natural abundance		90 %
3	D2O	natural abundance		10 %

9 3D (h)NNH HSQC-NOESY-HSQC

Instrument

Bruker Avance - 800 MHz

Sample

State isotropic, Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 310 K, pH 7.2, Details 1.05 mM ER690.005, 90% H2O/10% D2O

#	Name	Isotope labeling	Type	Concentration
1	ER690	natural abundance		1.05 mM
2	H20	natural abundance		90 %
3	D2O	natural abundance		10 %

10 3D (h)CCH HSQC-NOESY-HSQC

Instrument

Bruker Avance - 800 MHz

Sample

State isotropic, Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 310 K, pH 7.2, Details 1.05 mM ER690.005, 90% H2O/10% D2O

#	Name	Isotope labeling	Type	Concentration
1	ER690	natural abundance		1.05 mM
2	H20	natural abundance		90 %
3	D2O	natural abundance		10 %

11 3D (h)CNH HSQC-NOESY-HSQC

Instrument

Bruker Avance - 800 MHz

Sample

State isotropic, Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 310 K, pH 7.2, Details 1.05 mM ER690.005, 90% H2O/10% D2O

#	Name	Isotope labeling	Type	Concentration
1	ER690	natural abundance		1.05 mM
2	H20	natural abundance		90 %
3	D2O	natural abundance		10 %

12 3D (h)NCH HSQC-NOESY-HSQC

Instrument

Bruker Avance - 800 MHz

Sample

State isotropic, Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 310 K, pH 7.2, Details 1.05 mM ER690.005, 90% H2O/10% D2O

#	Name	Isotope labeling	Type	Concentration
1	ER690	natural abundance		1.05 mM
2	H20	natural abundance		90 %
3	D2O	natural abundance		10 %

13 2D 1H-13C HSQC

Instrument

Bruker Avance - 800 MHz

Sample

State isotropic, Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 310 K, pH 7.2, Details 1.05 mM ER690.005, 90% H2O/10% D2O

#	Name	Isotope labeling	Type	Concentration
1	ER690	natural abundance		1.05 mM
2	H20	natural abundance		90 %
3	D2O	natural abundance		10 %

nullKeywords Structural Genomics, Protein NMR, NORTHEAST STRUCTURAL GENOMICS CONSORTIUM (NESG), Target ER690, PSI-Biology, Protein Structure Initiative