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BMRB: 25242


25242
4V10
SKELEMIN ASSOCIATION WITH ALFA2B,BETTA3 INTEGRIN: A STRUCTURAL MODEL
Authors
Gorbatyuk, V., Deshmukh, L., Nguyen, K., Vinogradova, O.
Assembly
Sk45mNoTag
Entity
1. Sk45mNoTag (polymer, Thiol state: all free), 231 monomers, 27049.55 Da Detail

GSHMEEEMKR LLALSQEHKF PTVPTKSELA VEILEKGQVR FWMQAEKLSS NAKVSYIFNE KEIFEGPKYK MHIDRNTGII EMFMEKLQDE DEGTYTFQIQ DGKATGHSTL VLIGDVYKKL QKEAEFQRQE WIRKQGPHFA EYLSWEVTGE SNVLLKCKVA NIKKETHIVW YKDEREISVD EKHDFKDGIC TLLITEFSKK DAGFYEVILK DDRGKDKSRL KLVDEAFQDL M


Formula weight
27049.55 Da
Source organism
Mus musculus
Exptl. method
solution NMR
Refine. method
ARIA, XPLOR-NIH
Data set
assigned_chemical_shifts
Chem. Shift Complete1
Sequence coverage: 95.7 %, Completeness: 72.1 %, Completeness (bb): 69.4 % Detail
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Polymer type: polypeptide(L)

Total1H13C15N
All72.1 % (2049 of 2842)82.9 % (1238 of 1493)52.8 % (582 of 1102)92.7 % (229 of 247)
Backbone69.4 % (956 of 1378)89.2 % (420 of 471)48.2 % (328 of 680)91.6 % (208 of 227)
Sidechain71.8 % (1207 of 1682)79.1 % (808 of 1022)59.2 % (379 of 640)100.0 % (20 of 20)
Aromatic57.1 % (144 of 252)75.4 % (95 of 126)36.9 % (45 of 122)100.0 % (4 of 4)
Methyl98.7 % (227 of 230)98.3 % (113 of 115)99.1 % (114 of 115)

1. Sk45

GSHMEEEMKR LLALSQEHKF PTVPTKSELA VEILEKGQVR FWMQAEKLSS NAKVSYIFNE KEIFEGPKYK MHIDRNTGII EMFMEKLQDE DEGTYTFQIQ DGKATGHSTL VLIGDVYKKL QKEAEFQRQE WIRKQGPHFA EYLSWEVTGE SNVLLKCKVA NIKKETHIVW YKDEREISVD EKHDFKDGIC TLLITEFSKK DAGFYEVILK DDRGKDKSRL KLVDEAFQDL M

Show sample condition
Sample

Solvent system 90% WATER/10% D2O, Pressure 1.000 atm, Temperature 298.000 K, pH 6.800, Details 0.5 mM


#NameIsotope labelingTypeConcentration
1Sk45[U-13C; U-15N]0.5 mM
2potassium phosphatenatural abundance20 mM

Chem. Shift Complete2
Sequence coverage: 97.4 %, Completeness: 73.5 %, Completeness (bb): 72.0 % Detail
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Polymer type: polypeptide(L)

Total1H13C15N
All73.5 % (4175 of 5684)83.3 % (2486 of 2986)55.6 % (1225 of 2204)93.9 % (464 of 494)
Backbone72.0 % (1984 of 2756)90.8 % (855 of 942)52.0 % (707 of 1360)93.0 % (422 of 454)
Sidechain72.5 % (2439 of 3364)79.1 % (1616 of 2044)61.2 % (783 of 1280)100.0 % (40 of 40)
Aromatic60.3 % (304 of 504)75.0 % (189 of 252)43.9 % (107 of 244)100.0 % (8 of 8)
Methyl98.0 % (451 of 460)97.4 % (224 of 230)98.7 % (227 of 230)

1. Sk45

GSHMEEEMKR LLALSQEHKF PTVPTKSELA VEILEKGQVR FWMQAEKLSS NAKVSYIFNE KEIFEGPKYK MHIDRNTGII EMFMEKLQDE DEGTYTFQIQ DGKATGHSTL VLIGDVYKKL QKEAEFQRQE WIRKQGPHFA EYLSWEVTGE SNVLLKCKVA NIKKETHIVW YKDEREISVD EKHDFKDGIC TLLITEFSKK DAGFYEVILK DDRGKDKSRL KLVDEAFQDL M

Show sample condition
Sample

Solvent system 90% WATER/10% D2O, Pressure 1.000 atm, Temperature 298.000 K, pH 6.800, Details 0.5 mM


#NameIsotope labelingTypeConcentration
1Sk45[U-13C; U-15N]0.5 mM
2potassium phosphatenatural abundance20 mM

LACS Plot; CA
Referencing offset: -0.12 ppm, Outliers: 1 Detail
LACS Plot; CB
Referencing offset: -0.12 ppm, Outliers: 1 Detail
LACS Plot; HA
Referencing offset: -0.06 ppm, Outliers: 3 Detail
Protein Blocks Logo
Calculated from 20 models in PDB: 4V10, Strand ID: A Detail

Release date
2016-09-21
Citation
Skelemin association with αIIbβ3 integrin: a structural model
Gorbatyuk, V., Nguyen, K., Podolnikova, N.P., Deshmukh, L., Lin, X., Ugarova, T.P., Vinogradova, O.
Biochemistry (2014), 53, 6766-6775, PubMed 25224262 , DOI 10.1021/bi500680s ,
Related entities 1. Sk45mNoTag, : 1 : 2 : 44 entities Detail
More details for 47 related entities
Interaction partners 1. Sk45mNoTag, : 6 interactors Detail
More details for 6 interactors identified by 2 citations
Experiments performed 3 experiments Detail
nullKeywords STRUCTURAL PROTEIN, imminoglobulin C2-like domain, integrin, myomesin, skelemin