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BMRB: 25259

2MVB

NMR structure of hypothetical protein NP_344732.1 from Streptococcus pneumoniae TIGR4

Authors

Dutta, S.K., Serrano, P., Geralt, M., Wuthrich, K.

Assembly

hypothetical protein NP 344732.1 from Streptococcus pneumoniae TIGR4

Entity

1. hypothetical protein NP 344732.1 from Streptococcus pneumoniae TIGR4 (polymer, Thiol state: not present), 165 monomers, 18374.52 Da Detail

GPATKTEKDT LQSALPVIEN AEKNTVVTKT LVLPKSDDGS QQTQTITYKD KTFLSLAIQQ KRPVSDELKT YIDQHGVEET QKALLEAEEK DKSIIEARKL AGFKLETKLL SATELQTTTS FDFQVLDVKK ASQLEHLKNI GLENLLKNEP SKYISDRLAN GATEQ

Formula weight

18374.52 Da

Source organism

Streptococcus pneumoniae TIGR4

Exptl. method

solution NMR

Refine. method

molecular dynamics

Data set

assigned_chemical_shifts

Chem. Shift Complete

Sequence coverage: 100.0 %, Completeness: 90.1 %, Completeness (bb): 86.0 % Detail

Polymer type: polypeptide(L)

	Total	¹H	¹³C	¹⁵N
All	90.1 % (1750 of 1943)	96.3 % (977 of 1015)	79.7 % (598 of 750)	98.3 % (175 of 178)
Backbone	86.0 % (843 of 980)	99.7 % (330 of 331)	72.4 % (354 of 489)	99.4 % (159 of 160)
Sidechain	95.0 % (1066 of 1122)	94.6 % (647 of 684)	96.0 % (403 of 420)	88.9 % (16 of 18)
Aromatic	97.2 % (70 of 72)	97.2 % (35 of 36)	97.2 % (35 of 36)
Methyl	96.2 % (200 of 208)	97.1 % (101 of 104)	95.2 % (99 of 104)

1. entity

GPATKTEKDT LQSALPVIEN AEKNTVVTKT LVLPKSDDGS QQTQTITYKD KTFLSLAIQQ KRPVSDELKT YIDQHGVEET QKALLEAEEK DKSIIEARKL AGFKLETKLL SATELQTTTS FDFQVLDVKK ASQLEHLKNI GLENLLKNEP SKYISDRLAN GATEQ

Show sample condition

Sample

Solvent system 95% H2O/5% D2O, Pressure 1 atm, Temperature 298 K, pH 6.0

#	Name	Isotope labeling	Concentration
1	entity	[U-99% 13C; U-98% 15N]	1.2 mM
2	sodium phosphate	natural abundance	20 mM
3	sodium chloride	natural abundance	50 mM
4	sodium azide	natural abundance	5 mM
5	H2O	natural abundance	95 %
6	D2O	natural abundance	5 %

LACS Plot; CA

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹H	DSS	methyl protons	internal	direct	1.0
¹³C	DSS	methyl protons	na	indirect	0.2514495
¹⁵N	DSS	methyl protons	na	indirect	0.1013291

Referencing offset: 2.4 ppm, Outliers: 2 Detail

LACS Plot; CB

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹H	DSS	methyl protons	internal	direct	1.0
¹³C	DSS	methyl protons	na	indirect	0.2514495
¹⁵N	DSS	methyl protons	na	indirect	0.1013291

Referencing offset: 2.4 ppm, Outliers: 2 Detail

LACS Plot; HA

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹H	DSS	methyl protons	internal	direct	1.0
¹³C	DSS	methyl protons	na	indirect	0.2514495
¹⁵N	DSS	methyl protons	na	indirect	0.1013291

Referencing offset: -0.12 ppm, Outliers: 1 Detail

Protein Blocks Logo

Calculated from 20 models in PDB: 2MVB, Strand ID: A Detail

Release date

2014-11-02

Citation

NMR structure of hypothetical protein NP_344732.1 from Streptococcus pneumoniae TIGR4
Dutta, S.K., Serrano, P., Geralt, M., Wuthrich, K.
Not known

Related entities 1. hypothetical protein NP 344732.1 from Streptococcus pneumoniae TIGR4, : 1 entities Detail

Experiments performed 8 experiments Detail

1 APSY 4D-HACANH