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BMRB: 25263

6MNT

NMR assignments of the CUS-3 phage coat protein insertion domain.

Authors

Tripler, T.N., Maciejewski, M.W., Teschke, C.M., Alexandrescu, A.T.

Assembly

CUS-3id

Entity

1. CUS-3id (polymer, Thiol state: not present), 126 monomers, 13255.59 × 2 Da Detail

MASGSTESLT VSGQPEHKVE AKDSNGMPVD NRQGTITVSA SGLQVGDAFT IAGVNSVHQI TKDTTGQPQV FRVLAVSGTT VTISPKILPV ENTDVASRPY ANVDAKPAES AAITILNKLE HHHHHH

Total weight

26511.18 Da

Max. entity weight

13255.59 Da

Source organism

Bacteriophage sp.

Exptl. method

solution NMR

Refine. method

molecular dynamics

Data set

assigned_chemical_shifts

Chem. Shift Complete

Sequence coverage: 92.9 %, Completeness: 88.6 %, Completeness (bb): 91.5 % Detail

Polymer type: polypeptide(L)

	Total	¹H	¹³C	¹⁵N
All	88.6 % (1201 of 1356)	88.6 % (613 of 692)	87.6 % (467 of 533)	92.4 % (121 of 131)
Backbone	91.5 % (679 of 742)	90.9 % (231 of 254)	91.9 % (339 of 369)	91.6 % (109 of 119)
Sidechain	86.0 % (629 of 731)	87.2 % (382 of 438)	83.6 % (235 of 281)	100.0 % (12 of 12)
Aromatic	30.0 % (18 of 60)	60.0 % (18 of 30)	0.0 % (0 of 30)
Methyl	89.9 % (142 of 158)	84.8 % (67 of 79)	94.9 % (75 of 79)

1. CUS-3id

MASGSTESLT VSGQPEHKVE AKDSNGMPVD NRQGTITVSA SGLQVGDAFT IAGVNSVHQI TKDTTGQPQV FRVLAVSGTT VTISPKILPV ENTDVASRPY ANVDAKPAES AAITILNKLE HHHHHH

Show sample condition

Sample #1

Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 273 K, pH 6

#	Name	Isotope labeling	Concentration
1	insertion domain	[U-100% 15N]	1.9 mM
2	insertion domain	[U-100% 13C; U-100% 15N]	1.9 mM
3	H2O	natural abundance	90 %
4	D2O	natural abundance	10 %

Sample #2

Solvent system 100% D2O, Pressure 1 atm, Temperature 273 K, pH 6

#	Name	Isotope labeling	Type	Concentration
5	insertion domain	[U-100% 13C; U-100% 15N]		1.9 mM
6	D2O	natural abundance		100 %

LACS Plot; CA

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹³C	DSS	methyl protons	internal	indirect	0.2514495
¹H	DSS	methyl protons	internal	direct	1.0
¹⁵N	DSS	methyl protons	internal	indirect	0.1013291

Referencing offset: 0.34 ppm, Outliers: 3 Detail

LACS Plot; CB

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹³C	DSS	methyl protons	internal	indirect	0.2514495
¹H	DSS	methyl protons	internal	direct	1.0
¹⁵N	DSS	methyl protons	internal	indirect	0.1013291

Referencing offset: 0.34 ppm, Outliers: 3 Detail

LACS Plot; HA

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹³C	DSS	methyl protons	internal	indirect	0.2514495
¹H	DSS	methyl protons	internal	direct	1.0
¹⁵N	DSS	methyl protons	internal	indirect	0.1013291

Referencing offset: -0.1 ppm, Outliers: 4 Detail

LACS Plot; CO

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹³C	DSS	methyl protons	internal	indirect	0.2514495
¹H	DSS	methyl protons	internal	direct	1.0
¹⁵N	DSS	methyl protons	internal	indirect	0.1013291

Referencing offset: 2.35 ppm, Outliers: 3 Detail

Protein Blocks Logo

Calculated from 25 models in PDB: 6MNT, Strand ID: A Detail

Release date

2015-08-06

Citation

NMR assignments for the insertion domain of bacteriophage CUS-3 coat protein
Tripler, T.N., Maciejewski, M.W., Teschke, C.M., Alexandrescu, A.T.
Biomol. NMR Assign. (2015), 9, 333-336, PubMed 25694158 , DOI 10.1007/s12104-015-9604-4 , Abstract

Related entities 1. CUS-3id, : 1 : 8 entities Detail

Experiments performed 12 experiments Detail

1 2D 1H-15N HSQC