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BMRB: 25265

2MVF

Structural insight into an essential assembly factor network on the pre-ribosome

Authors

Lee, W., BaBler, J., Paternoga, H., Holdermann, I., Thomas, M., Granneman, S., Barrio-Garcia, C., Nyarko, A., Stier, G., Clark, S.A., Schraivogel, D., Kallas, M., Beckmann, R., Tollervey, D., Barbar, E., Sinning, I., Hurt, E.

Assembly

Uncharacterized protein

Entity

1. Uncharacterized protein (polymer, Thiol state: all free), 188 monomers, 20595.73 Da Detail

YERFIRPMGL RYKKANVTHP TLNVTVQLPI LSVKKNPSNP LYTQLGVLTK GTIIEVNVSD LGIVTASGKI AWGRYAQITN NPENDGCVNA VLLVYERFIR PMGLRYKKAN VTHPTLNVTV QLPILSVKKN PSNPLYTQLG VLTKGTIIEV NVSDLGIVTA SGKIAWGRYA QITNNPENDG CVNAVLLV

Formula weight

20595.73 Da

Source organism

Chaetomium thermophilum

Exptl. method

solution NMR

Refine. method

simulated annealing

Data set

assigned_chemical_shifts

Chem. Shift Complete

Sequence coverage: 59.6 %, Completeness: 49.5 %, Completeness (bb): 56.1 % Detail

Polymer type: polypeptide(L)

	Total	¹H	¹³C	¹⁵N
All	49.5 % (1089 of 2198)	49.2 % (559 of 1136)	49.5 % (426 of 860)	51.5 % (104 of 202)
Backbone	56.1 % (619 of 1104)	57.4 % (217 of 378)	54.5 % (300 of 550)	58.0 % (102 of 176)
Sidechain	44.6 % (566 of 1268)	45.1 % (342 of 758)	45.9 % (222 of 484)	7.7 % (2 of 26)
Aromatic	21.6 % (25 of 116)	24.1 % (14 of 58)	19.6 % (11 of 56)	0.0 % (0 of 2)
Methyl	48.9 % (135 of 276)	48.6 % (67 of 138)	49.3 % (68 of 138)

1. entity

Show sample condition

Sample

Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 280 K, pH 7.4

#	Name	Isotope labeling	Concentration
1	D2O	natural abundance	10 %
2	TRIS	natural abundance	10 mM
3	H2O	natural abundance	90 %
4	sodium chloride	natural abundance	5 mM
5	DSS	natural abundance	1 uM
6	TCEP	natural abundance	2 mM
7	entity	[U-99% 13C; U-99% 15N]	450 mM

LACS Plot; CA

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹³C	DSS	methyl protons	na	indirect	0.2514495
¹H	DSS	methyl protons	internal	direct	1.0
¹⁵N	DSS	methyl protons	na	indirect	0.1013291

Referencing offset: -0.18 ppm, Outliers: 2 Detail

LACS Plot; CB

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹³C	DSS	methyl protons	na	indirect	0.2514495
¹H	DSS	methyl protons	internal	direct	1.0
¹⁵N	DSS	methyl protons	na	indirect	0.1013291

Referencing offset: -0.18 ppm, Outliers: 2 Detail

LACS Plot; HA

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹³C	DSS	methyl protons	na	indirect	0.2514495
¹H	DSS	methyl protons	internal	direct	1.0
¹⁵N	DSS	methyl protons	na	indirect	0.1013291

Referencing offset: -0.08 ppm, Outliers: 2 Detail

LACS Plot; CO

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹³C	DSS	methyl protons	na	indirect	0.2514495
¹H	DSS	methyl protons	internal	direct	1.0
¹⁵N	DSS	methyl protons	na	indirect	0.1013291

Referencing offset: 0.31 ppm, Outliers: 1 Detail

Protein Blocks Logo

Calculated from 20 models in PDB: 2MVF, Strand ID: A Detail

Release date

2015-08-24

Citation

A network of assembly factors is involved in remodeling rRNA elements during preribosome maturation
Lee, W., BaBler, J., Paternoga, H., Holdermann, I., Thomas, M., Granneman, S., Barrio-Garcia, C., Nyarko, A., Stier, G., Clark, S.A., Schraivogel, D., Kallas, M., Beckmann, R., Tollervey, D., Barbar, E., Sinning, I., Hurt, E.
J. Cell Biol. (2014), 207, 481-498, PubMed 25404745 , DOI 10.1083/jcb.201408111 , Abstract

Experiments performed 11 experiments Detail

1 2D 1H-15N HSQC