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BMRB: 25266

2MVG

Solution structure of decorin binding protein B from Borrelia burgdorferi

Authors

Wang, X., Feng, W.

Assembly

decorin binding protein B

Entity

1. decorin binding protein B (polymer, Thiol state: not present), 167 monomers, 18180.91 Da Detail

SSIGLVERTN AALESSSKDL KNKILKIKKE ATGKGVLFEA FTGLKTGSKV TSGGLALREA KVQAIVETGK FLKIIEEEAL KLKETGNSGQ FLAMFDLMLE VVESLEDVGI IGLKARVLEE SKNNPINTAE RLLAAKAQIE NQLKVVKEKQ NIENGGEKKN NKSKKKK

Formula weight

18180.91 Da

Source organism

Borreliella burgdorferi

Exptl. method

solution NMR

Refine. method

simulated annealing

Data set

assigned_chemical_shifts

Chem. Shift Complete

Sequence coverage: 99.4 %, Completeness: 85.6 %, Completeness (bb): 96.6 % Detail

Polymer type: polypeptide(L)

	Total	¹H	¹³C	¹⁵N
All	85.6 % (1695 of 1979)	78.0 % (813 of 1042)	93.6 % (707 of 755)	96.2 % (175 of 182)
Backbone	96.6 % (966 of 1000)	95.1 % (330 of 347)	97.3 % (474 of 487)	97.6 % (162 of 166)
Sidechain	77.6 % (878 of 1132)	69.5 % (483 of 695)	90.7 % (382 of 421)	81.3 % (13 of 16)
Aromatic	16.0 % (8 of 50)	32.0 % (8 of 25)	0.0 % (0 of 25)
Methyl	97.6 % (207 of 212)	97.2 % (103 of 106)	98.1 % (104 of 106)

1. DBPB

SSIGLVERTN AALESSSKDL KNKILKIKKE ATGKGVLFEA FTGLKTGSKV TSGGLALREA KVQAIVETGK FLKIIEEEAL KLKETGNSGQ FLAMFDLMLE VVESLEDVGI IGLKARVLEE SKNNPINTAE RLLAAKAQIE NQLKVVKEKQ NIENGGEKKN NKSKKKK

Show sample condition

Sample

Solvent system 95% H2O/5% D2O, Pressure 1 atm, Temperature 298 K, pH 5.0

#	Name	Isotope labeling	Concentration
1	DBPB	[U-100% 13C; U-100% 15N]	0.8 mM
2	sodium phosphate	natural abundance	50 mM
3	sodium chloride	natural abundance	150 mM
4	H2O	natural abundance	95 %
5	D2O	natural abundance	5 %

LACS Plot; CA

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹³C	DSS	methyl protons	na	indirect	0.2514495
¹H	DSS	methyl protons	internal	direct	1.0
¹⁵N	DSS	methyl protons	na	indirect	0.1013291

Referencing offset: -0.12 ppm, Outliers: 1 Detail

LACS Plot; CB

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹³C	DSS	methyl protons	na	indirect	0.2514495
¹H	DSS	methyl protons	internal	direct	1.0
¹⁵N	DSS	methyl protons	na	indirect	0.1013291

Referencing offset: -0.12 ppm, Outliers: 1 Detail

LACS Plot; HA

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹³C	DSS	methyl protons	na	indirect	0.2514495
¹H	DSS	methyl protons	internal	direct	1.0
¹⁵N	DSS	methyl protons	na	indirect	0.1013291

Referencing offset: -0.02 ppm, Outliers: 1 Detail

LACS Plot; CO

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹³C	DSS	methyl protons	na	indirect	0.2514495
¹H	DSS	methyl protons	internal	direct	1.0
¹⁵N	DSS	methyl protons	na	indirect	0.1013291

Referencing offset: -2.09 ppm, Outliers: 1 Detail

Protein Blocks Logo

Calculated from 10 models in PDB: 2MVG, Strand ID: A Detail

Release date

2015-08-16

Citation

Structure of Decorin Binding Protein B from Borrelia Spirochetes
Wang, X., Feng, W., Chao, A.
Not known

Related entities 1. decorin binding protein B, : 1 : 2 : 5 entities Detail

Experiments performed 9 experiments Detail

1 2D 1H-15N HSQC