Tetramerization domain of the Ciona intestinalis p53/p73-b transcription factor protein
Polymer type: polypeptide(L)
Total | 1H | 13C | 15N | |
---|---|---|---|---|
All | 97.7 % (564 of 577) | 97.7 % (299 of 306) | 99.1 % (218 of 220) | 92.2 % (47 of 51) |
Backbone | 98.6 % (276 of 280) | 98.9 % (94 of 95) | 98.6 % (137 of 139) | 97.8 % (45 of 46) |
Sidechain | 97.4 % (333 of 342) | 97.2 % (205 of 211) | 100.0 % (126 of 126) | 40.0 % (2 of 5) |
Aromatic | 100.0 % (34 of 34) | 100.0 % (17 of 17) | 100.0 % (17 of 17) | |
Methyl | 100.0 % (50 of 50) | 100.0 % (25 of 25) | 100.0 % (25 of 25) |
1. entity
SDGDVVYTLN IRGKRKFEKV KEYKEALDLL DYVQPDVKKA CCQRNQISolvent system 95% H2O/5% D2O, Pressure 1 atm, Temperature 298 K, pH 7.0
# | Name | Isotope labeling | Type | Concentration |
---|---|---|---|---|
1 | entity | [U-100% 15N] | 2.5 mM | |
2 | L-arginine | natural abundance | 50 mM | |
3 | L-glutamate | natural abundance | 50 mM | |
4 | DTT | natural abundance | 1 mM | |
5 | H2O | natural abundance | 95 % | |
6 | D2O | natural abundance | 5 % |
Solvent system 95% H2O/5% D2O, Pressure 1 atm, Temperature 298 K, pH 7.0
# | Name | Isotope labeling | Type | Concentration |
---|---|---|---|---|
7 | entity | [U-100% 13C; U-100% 15N] | 2.5 mM | |
8 | L-arginine | natural abundance | 50 mM | |
9 | L-glutamate | natural abundance | 50 mM | |
10 | DTT | natural abundance | 1 mM | |
11 | H2O | natural abundance | 95 % | |
12 | D2O | natural abundance | 5 % |
Solvent system 95% H2O/5% D2O, Pressure 1 atm, Temperature 298 K, pH 7.0
# | Name | Isotope labeling | Type | Concentration |
---|---|---|---|---|
13 | entity | [U-100% 15N] | 2 mM | |
14 | entity | [U-100% 13C] | 2 mM | |
15 | L-arginine | natural abundance | 50 mM | |
16 | L-glutamate | natural abundance | 50 mM | |
17 | DTT | natural abundance | 1 mM | |
18 | H2O | natural abundance | 95 % | |
19 | D2O | natural abundance | 5 % |
Bruker Avance - 900 MHz CryoProbe 1H,13C,15N
State isotropic, Solvent system 95% H2O/5% D2O, Pressure 1 atm, Temperature 298 K, pH 7.0
# | Name | Isotope labeling | Type | Concentration |
---|---|---|---|---|
1 | entity | [U-100% 15N] | 2.5 mM | |
2 | L-arginine | natural abundance | 50 mM | |
3 | L-glutamate | natural abundance | 50 mM | |
4 | DTT | natural abundance | 1 mM | |
5 | H2O | natural abundance | 95 % | |
6 | D2O | natural abundance | 5 % |
Bruker Avance - 800 MHz CryoProbe 1H,13C,15N
State isotropic, Solvent system 95% H2O/5% D2O, Pressure 1 atm, Temperature 298 K, pH 7.0
# | Name | Isotope labeling | Type | Concentration |
---|---|---|---|---|
7 | entity | [U-100% 13C; U-100% 15N] | 2.5 mM | |
8 | L-arginine | natural abundance | 50 mM | |
9 | L-glutamate | natural abundance | 50 mM | |
10 | DTT | natural abundance | 1 mM | |
11 | H2O | natural abundance | 95 % | |
12 | D2O | natural abundance | 5 % |
Bruker Avance - 900 MHz CryoProbe 1H,13C,15N
State isotropic, Solvent system 95% H2O/5% D2O, Pressure 1 atm, Temperature 298 K, pH 7.0
# | Name | Isotope labeling | Type | Concentration |
---|---|---|---|---|
7 | entity | [U-100% 13C; U-100% 15N] | 2.5 mM | |
8 | L-arginine | natural abundance | 50 mM | |
9 | L-glutamate | natural abundance | 50 mM | |
10 | DTT | natural abundance | 1 mM | |
11 | H2O | natural abundance | 95 % | |
12 | D2O | natural abundance | 5 % |
Bruker Avance - 500 MHz
State isotropic, Solvent system 95% H2O/5% D2O, Pressure 1 atm, Temperature 298 K, pH 7.0
# | Name | Isotope labeling | Type | Concentration |
---|---|---|---|---|
7 | entity | [U-100% 13C; U-100% 15N] | 2.5 mM | |
8 | L-arginine | natural abundance | 50 mM | |
9 | L-glutamate | natural abundance | 50 mM | |
10 | DTT | natural abundance | 1 mM | |
11 | H2O | natural abundance | 95 % | |
12 | D2O | natural abundance | 5 % |
Bruker Avance - 500 MHz
State isotropic, Solvent system 95% H2O/5% D2O, Pressure 1 atm, Temperature 298 K, pH 7.0
# | Name | Isotope labeling | Type | Concentration |
---|---|---|---|---|
7 | entity | [U-100% 13C; U-100% 15N] | 2.5 mM | |
8 | L-arginine | natural abundance | 50 mM | |
9 | L-glutamate | natural abundance | 50 mM | |
10 | DTT | natural abundance | 1 mM | |
11 | H2O | natural abundance | 95 % | |
12 | D2O | natural abundance | 5 % |
Bruker Avance - 600 MHz CryoProbe 1H,13C,15N
State isotropic, Solvent system 95% H2O/5% D2O, Pressure 1 atm, Temperature 298 K, pH 7.0
# | Name | Isotope labeling | Type | Concentration |
---|---|---|---|---|
7 | entity | [U-100% 13C; U-100% 15N] | 2.5 mM | |
8 | L-arginine | natural abundance | 50 mM | |
9 | L-glutamate | natural abundance | 50 mM | |
10 | DTT | natural abundance | 1 mM | |
11 | H2O | natural abundance | 95 % | |
12 | D2O | natural abundance | 5 % |
Bruker Avance - 500 MHz
State isotropic, Solvent system 95% H2O/5% D2O, Pressure 1 atm, Temperature 298 K, pH 7.0
# | Name | Isotope labeling | Type | Concentration |
---|---|---|---|---|
7 | entity | [U-100% 13C; U-100% 15N] | 2.5 mM | |
8 | L-arginine | natural abundance | 50 mM | |
9 | L-glutamate | natural abundance | 50 mM | |
10 | DTT | natural abundance | 1 mM | |
11 | H2O | natural abundance | 95 % | |
12 | D2O | natural abundance | 5 % |
Bruker Avance - 900 MHz CryoProbe 1H,13C,15N
State isotropic, Solvent system 95% H2O/5% D2O, Pressure 1 atm, Temperature 298 K, pH 7.0
# | Name | Isotope labeling | Type | Concentration |
---|---|---|---|---|
7 | entity | [U-100% 13C; U-100% 15N] | 2.5 mM | |
8 | L-arginine | natural abundance | 50 mM | |
9 | L-glutamate | natural abundance | 50 mM | |
10 | DTT | natural abundance | 1 mM | |
11 | H2O | natural abundance | 95 % | |
12 | D2O | natural abundance | 5 % |
Bruker Avance - 600 MHz CryoProbe 1H,13C,15N
State isotropic, Solvent system 95% H2O/5% D2O, Pressure 1 atm, Temperature 298 K, pH 7.0
# | Name | Isotope labeling | Type | Concentration |
---|---|---|---|---|
13 | entity | [U-100% 15N] | 2 mM | |
14 | entity | [U-100% 13C] | 2 mM | |
15 | L-arginine | natural abundance | 50 mM | |
16 | L-glutamate | natural abundance | 50 mM | |
17 | DTT | natural abundance | 1 mM | |
18 | H2O | natural abundance | 95 % | |
19 | D2O | natural abundance | 5 % |
Bruker Avance - 800 MHz CryoProbe 1H,13C,15N
State isotropic, Solvent system 95% H2O/5% D2O, Pressure 1 atm, Temperature 298 K, pH 7.0
# | Name | Isotope labeling | Type | Concentration |
---|---|---|---|---|
13 | entity | [U-100% 15N] | 2 mM | |
14 | entity | [U-100% 13C] | 2 mM | |
15 | L-arginine | natural abundance | 50 mM | |
16 | L-glutamate | natural abundance | 50 mM | |
17 | DTT | natural abundance | 1 mM | |
18 | H2O | natural abundance | 95 % | |
19 | D2O | natural abundance | 5 % |
Bruker Avance - 900 MHz CryoProbe 1H,13C,15N
State isotropic, Solvent system 95% H2O/5% D2O, Pressure 1 atm, Temperature 298 K, pH 7.0
# | Name | Isotope labeling | Type | Concentration |
---|---|---|---|---|
13 | entity | [U-100% 15N] | 2 mM | |
14 | entity | [U-100% 13C] | 2 mM | |
15 | L-arginine | natural abundance | 50 mM | |
16 | L-glutamate | natural abundance | 50 mM | |
17 | DTT | natural abundance | 1 mM | |
18 | H2O | natural abundance | 95 % | |
19 | D2O | natural abundance | 5 % |
Bruker Avance - 700 MHz CryoProbe 1H,13C,15N
State isotropic, Solvent system 95% H2O/5% D2O, Pressure 1 atm, Temperature 298 K, pH 7.0
# | Name | Isotope labeling | Type | Concentration |
---|---|---|---|---|
13 | entity | [U-100% 15N] | 2 mM | |
14 | entity | [U-100% 13C] | 2 mM | |
15 | L-arginine | natural abundance | 50 mM | |
16 | L-glutamate | natural abundance | 50 mM | |
17 | DTT | natural abundance | 1 mM | |
18 | H2O | natural abundance | 95 % | |
19 | D2O | natural abundance | 5 % |
Bruker Avance - 800 MHz CryoProbe 1H,13C,15N
State isotropic, Solvent system 95% H2O/5% D2O, Pressure 1 atm, Temperature 298 K, pH 7.0
# | Name | Isotope labeling | Type | Concentration |
---|---|---|---|---|
1 | entity | [U-100% 15N] | 2.5 mM | |
2 | L-arginine | natural abundance | 50 mM | |
3 | L-glutamate | natural abundance | 50 mM | |
4 | DTT | natural abundance | 1 mM | |
5 | H2O | natural abundance | 95 % | |
6 | D2O | natural abundance | 5 % |
Properties
Input source #1: NMR data (NEF) - Assigned chemical shifts, Distance restraints, Dihedral angle restraints | combined_25298_2mw4.nef |
Input source #2: Coordindates | 2mw4.cif |
Diamagnetism of the molecular assembly | True (excluding Oxygen atoms) |
Whether the assembly has a disulfide bond | None |
Whether the assembly has a other bond | None |
Whether the assembly contains a cyclic polymer | None |
Overall data processing status | Warning |
Disulfide bonds
NoneOther bonds (neither disulfide, covalent nor hydrogen bonds, e.g. Zinc–sulphur bond)
NoneNon-standard residues
NoneSequence alignments
-----110-------120-------130-------140--------- SDGDVVYTLNIRGKRKFEKVKEYKEALDLLDYVQPDVKKACCQRNQI ||||||||||||||||||||||||||||||||||||||||||||||| SDGDVVYTLNIRGKRKFEKVKEYKEALDLLDYVQPDVKKACCQRNQI --------10--------20--------30--------40-------
-----210-------220-------230-------240--------- SDGDVVYTLNIRGKRKFEKVKEYKEALDLLDYVQPDVKKACCQRNQI ||||||||||||||||||||||||||||||||||||||||||||||| SDGDVVYTLNIRGKRKFEKVKEYKEALDLLDYVQPDVKKACCQRNQI --------10--------20--------30--------40-------
-----310-------320-------330-------340--------- SDGDVVYTLNIRGKRKFEKVKEYKEALDLLDYVQPDVKKACCQRNQI ||||||||||||||||||||||||||||||||||||||||||||||| SDGDVVYTLNIRGKRKFEKVKEYKEALDLLDYVQPDVKKACCQRNQI --------10--------20--------30--------40-------
-----410-------420-------430-------440--------- SDGDVVYTLNIRGKRKFEKVKEYKEALDLLDYVQPDVKKACCQRNQI ||||||||||||||||||||||||||||||||||||||||||||||| SDGDVVYTLNIRGKRKFEKVKEYKEALDLLDYVQPDVKKACCQRNQI --------10--------20--------30--------40-------
Chain assignments
Entity_assembly_ID (NMR) | Auth_asym_ID (model) | Length | Unmapped | Conflict | Sequence coverage (%) |
---|---|---|---|---|---|
A | A | 47 | 0 | 0 | 100.0 |
B | B | 47 | 0 | 0 | 100.0 |
C | C | 47 | 0 | 0 | 100.0 |
D | D | 47 | 0 | 0 | 100.0 |
Content subtype: combined_25298_2mw4.nef
Assigned chemical shifts
-----110-------120-------130-------140--------- SDGDVVYTLNIRGKRKFEKVKEYKEALDLLDYVQPDVKKACCQRNQI ||||||||||||||||||||||||||||||||||||||||||||||| SDGDVVYTLNIRGKRKFEKVKEYKEALDLLDYVQPDVKKACCQRNQI
Atom group | # of target shifts | # of assigned shifts | Completeness (%) |
---|---|---|---|
1H chemical shifts | 306 | 297 | 97.1 |
13C chemical shifts | 220 | 218 | 99.1 |
15N chemical shifts | 54 | 46 | 85.2 |
Atom group | # of target shifts | # of assigned shifts | Completeness (%) |
---|---|---|---|
1H chemical shifts | 95 | 94 | 98.9 |
13C chemical shifts | 94 | 92 | 97.9 |
15N chemical shifts | 46 | 45 | 97.8 |
Atom group | # of target shifts | # of assigned shifts | Completeness (%) |
---|---|---|---|
1H chemical shifts | 211 | 203 | 96.2 |
13C chemical shifts | 126 | 126 | 100.0 |
15N chemical shifts | 8 | 1 | 12.5 |
Atom group | # of target shifts | # of assigned shifts | Completeness (%) |
---|---|---|---|
1H chemical shifts | 25 | 25 | 100.0 |
13C chemical shifts | 25 | 25 | 100.0 |
Atom group | # of target shifts | # of assigned shifts | Completeness (%) |
---|---|---|---|
1H chemical shifts | 17 | 17 | 100.0 |
13C chemical shifts | 17 | 17 | 100.0 |
Distance restraints
-----110-------120-------130-------140--------- SDGDVVYTLNIRGKRKFEKVKEYKEALDLLDYVQPDVKKACCQRNQI ||||||||||||||||||||||||||||||||||||||||||||||| SDGDVVYTLNIRGKRKFEKVKEYKEALDLLDYVQPDVKKACCQRNQI
-----110-------120-------130-------140--------- SDGDVVYTLNIRGKRKFEKVKEYKEALDLLDYVQPDVKKACCQRNQI | | | ||||||||||||||||| |||||||| ......Y.L.I...RKFEKVKEYKEALDLLD....DVKKACCQ -----110-------120-------130-------140-----
Dihedral angle restraints
-----110-------120-------130-------140--------- SDGDVVYTLNIRGKRKFEKVKEYKEALDLLDYVQPDVKKACCQRNQI ||||||| ||||||||||||||||||| |||||||||| .....VYTLNIR.KRKFEKVKEYKEALDLLDY..PDVKKACCQR -----110-------120-------130-------140------