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Tetramerization domain of the Ciona intestinalis p53/p73-b transcription factor protein
Authors
Heering, J.P., Jonker, H.R.A., Loehr, F., Schwalbe, H., Doetsch, V.
Assembly
Tetramerization domain of the Ciona intestinalis p53/p73-b transcription factor protein
Entity
1. Tetramerization domain of the Ciona intestinalis p53/p73-b transcription factor protein (polymer, Thiol state: all free), 47 monomers, 5518.282 × 4 Da Detail

SDGDVVYTLN IRGKRKFEKV KEYKEALDLL DYVQPDVKKA CCQRNQI


Total weight
22073.129 Da
Max. entity weight
5518.282 Da
Source organism
Ciona intestinalis
Exptl. method
solution NMR
Refine. method
simulated annealing, energy minimization
Data set
assigned_chemical_shifts
Chem. Shift Complete
Sequence coverage: 100.0 %, Completeness: 97.7 %, Completeness (bb): 98.6 % Detail

Polymer type: polypeptide(L)

Total1H13C15N
All97.7 % (564 of 577)97.7 % (299 of 306)99.1 % (218 of 220)92.2 % (47 of 51)
Backbone98.6 % (276 of 280)98.9 % (94 of 95)98.6 % (137 of 139)97.8 % (45 of 46)
Sidechain97.4 % (333 of 342)97.2 % (205 of 211)100.0 % (126 of 126)40.0 % (2 of 5)
Aromatic100.0 % (34 of 34)100.0 % (17 of 17)100.0 % (17 of 17)
Methyl100.0 % (50 of 50)100.0 % (25 of 25)100.0 % (25 of 25)

1. entity

SDGDVVYTLN IRGKRKFEKV KEYKEALDLL DYVQPDVKKA CCQRNQI

Sample #1

Solvent system 95% H2O/5% D2O, Pressure 1 atm, Temperature 298 K, pH 7.0


#NameIsotope labelingTypeConcentration
1entity[U-100% 15N]2.5 mM
2L-argininenatural abundance50 mM
3L-glutamatenatural abundance50 mM
4DTTnatural abundance1 mM
5H2Onatural abundance95 %
6D2Onatural abundance5 %
Sample #2

Solvent system 95% H2O/5% D2O, Pressure 1 atm, Temperature 298 K, pH 7.0


#NameIsotope labelingTypeConcentration
7entity[U-100% 13C; U-100% 15N]2.5 mM
8L-argininenatural abundance50 mM
9L-glutamatenatural abundance50 mM
10DTTnatural abundance1 mM
11H2Onatural abundance95 %
12D2Onatural abundance5 %
Sample #3

Solvent system 95% H2O/5% D2O, Pressure 1 atm, Temperature 298 K, pH 7.0


#NameIsotope labelingTypeConcentration
13entity[U-100% 15N]2 mM
14entity[U-100% 13C]2 mM
15L-argininenatural abundance50 mM
16L-glutamatenatural abundance50 mM
17DTTnatural abundance1 mM
18H2Onatural abundance95 %
19D2Onatural abundance5 %

Protein Blocks Logo
Calculated from 20 models in PDB: 2MW4, Strand ID: A, B, C, D Detail


Release date
2015-10-25
Citation
Structural investigations of the p53/p73 homologs from the tunicate species Ciona intestinalis reveal the sequence requirements for the formation of a tetramerization domain
Heering, J.P., Jonker, H.R.A., Loehr, F., Schwalbe, H., Doetsch, V.
Protein Sci. (2015), 25, 410-422, PubMed 26473758 , DOI 10.1002/pro.2830 ,
Related entities 1. Tetramerization domain of the Ciona intestinalis p53/p73-b transcription factor protein, : 1 : 13 entities Detail
Experiments performed 13 experiments Detail
NMR combined restraints 5 contents Detail
Keywords Ciona intestinalis, p53/p73-b, tetramerization domain, transcription factor