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BMRB: 25299

2MW5

Backbone fold of Human Small Ubiquitin like Modifier protein-1 (SUMO-1) based on Prot3D-NMR approach.

Authors

Kumar, D., jaiswal, N., raikwal, N., Shukla, V., Arora, A.

Assembly

Human Small Ubiquitin like Modifier protein-1 (SUMO-1)

Entity

1. Human Small Ubiquitin like Modifier protein-1 (SUMO-1) (polymer, Thiol state: all free), 97 monomers, 11132.40 Da Detail

MSDQEAKPST EDLGDKKEGE YIKLKVIGQD SSEIHFKVKM TTHLKKLKES YCQRQGVPMN SLRFLFEGQR IADNHTPKEL GMEEEDVIEV YQEQTGG

Formula weight

11132.4 Da

Source organism

Homo sapiens

Exptl. method

solution NMR

Refine. method

distance geometry

Data set

assigned_chemical_shifts

Chem. Shift Complete

Sequence coverage: 100.0 %, Completeness: 78.0 %, Completeness (bb): 96.9 % Detail

Polymer type: polypeptide(L)

	Total	¹H	¹³C	¹⁵N
All	78.0 % (901 of 1155)	69.3 % (424 of 612)	87.0 % (383 of 440)	91.3 % (94 of 103)
Backbone	96.9 % (558 of 576)	94.5 % (188 of 199)	97.9 % (277 of 283)	98.9 % (93 of 94)
Sidechain	64.7 % (432 of 668)	57.1 % (236 of 413)	79.3 % (195 of 246)	11.1 % (1 of 9)
Aromatic	3.0 % (2 of 66)	6.1 % (2 of 33)	0.0 % (0 of 33)
Methyl	74.4 % (61 of 82)	63.4 % (26 of 41)	85.4 % (35 of 41)

1. entity

MSDQEAKPST EDLGDKKEGE YIKLKVIGQD SSEIHFKVKM TTHLKKLKES YCQRQGVPMN SLRFLFEGQR IADNHTPKEL GMEEEDVIEV YQEQTGG

Show sample condition

Sample

Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 298 K, pH 6.5, Details 1mM 13C-15N labelled protein Sample

#	Name	Isotope labeling	Type	Concentration
1	entity	[U-100% 13C; U-100% 15N]		0.9 (±0.1) mM
2	sodium phosphate	natural abundance		200 mM

LACS Plot; CA

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹H	DSS	methyl protons	internal	direct	1.0
¹³C	DSS	methyl protons	na	indirect	0.2514495
¹⁵N	DSS	methyl protons	na	indirect	0.1013291

Referencing offset: -0.03 ppm, Outliers: 2 Detail

LACS Plot; CB

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹H	DSS	methyl protons	internal	direct	1.0
¹³C	DSS	methyl protons	na	indirect	0.2514495
¹⁵N	DSS	methyl protons	na	indirect	0.1013291

Referencing offset: -0.03 ppm, Outliers: 2 Detail

LACS Plot; HA

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹H	DSS	methyl protons	internal	direct	1.0
¹³C	DSS	methyl protons	na	indirect	0.2514495
¹⁵N	DSS	methyl protons	na	indirect	0.1013291

Referencing offset: -0.1 ppm, Outliers: 1 Detail

LACS Plot; CO

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹H	DSS	methyl protons	internal	direct	1.0
¹³C	DSS	methyl protons	na	indirect	0.2514495
¹⁵N	DSS	methyl protons	na	indirect	0.1013291

Referencing offset: 0.56 ppm, Outliers: 1 Detail

Protein Blocks Logo

Calculated from 10 models in PDB: 2MW5, Strand ID: A Detail

Release date

2014-11-30

Citation

Prot3DNMR: A simple and swift NMR strategy for Three-Dimentional structutral determination of proteins
Kumar, D., jaiswal, N., raikwal, N., Shukla, V.KUMAR., Arora, A.
Not known

Related entities 1. Human Small Ubiquitin like Modifier protein-1 (SUMO-1), : 1 : 19 : 41 : 126 entities Detail

Interaction partners 1. Human Small Ubiquitin like Modifier protein-1 (SUMO-1), : 123 interactors Detail

More details for 123 interactors identified by 62 citations

Experiments performed 7 experiments Detail

1 2D 1H-15N HSQC