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BMRB: 25342

2MWM

NMR structure of the protein YP_193882.1 from Lactobacillus acidophilus NCFM in presence of FMN

Authors

Dutta, S.K., Serrano, P., Geralt, M., Wuthrich, K.

Assembly

NMR structure of the protein YP 193882.1 from Lactobacillus acidophilus NCFM in presence of FMN

Entity

1. NMR structure of the protein YP 193882.1 from Lactobacillus acidophilus NCFM in presence of FMN, entity (polymer, Thiol state: all free), 151 monomers, 17059.88 Da Detail

GMAKKTLILY YSWSGETKKM AEKINSEIKD SELKEVKVSE GTFDADMYKT SDIALDQIQG NKDFPEIQLD NIDYNNYDLI LIGSPVWSGY PATPIKTLLD QMKNYRGEVA SFFTSAGTNH KAYVSHFNEW ADGLNVIGVA RDDSEVDKWS K

2. FLAVIN MONONUCLEOTIDE (non-polymer), 456.344 Da

Total weight

17516.225 Da

Max. entity weight

17059.88 Da

Source organism

Lactobacillus acidophilus NCFM

Exptl. method

solution NMR

Refine. method

molecular dynamics

Data set

assigned_chemical_shifts

Chem. Shift Complete

Sequence coverage: 98.7 %, Completeness: 92.6 %, Completeness (bb): 96.1 % Detail

Polymer type: polypeptide(L)

	Total	¹H	¹³C	¹⁵N
All	92.6 % (1641 of 1772)	94.1 % (864 of 918)	89.3 % (616 of 690)	98.2 % (161 of 164)
Backbone	96.1 % (863 of 898)	96.8 % (298 of 308)	95.0 % (421 of 443)	98.0 % (144 of 147)
Sidechain	90.4 % (918 of 1015)	92.8 % (566 of 610)	86.3 % (335 of 388)	100.0 % (17 of 17)
Aromatic	53.5 % (91 of 170)	62.4 % (53 of 85)	42.0 % (34 of 81)	100.0 % (4 of 4)
Methyl	98.7 % (150 of 152)	100.0 % (76 of 76)	97.4 % (74 of 76)

1. entity

GMAKKTLILY YSWSGETKKM AEKINSEIKD SELKEVKVSE GTFDADMYKT SDIALDQIQG NKDFPEIQLD NIDYNNYDLI LIGSPVWSGY PATPIKTLLD QMKNYRGEVA SFFTSAGTNH KAYVSHFNEW ADGLNVIGVA RDDSEVDKWS K

Show sample condition

Sample

Solvent system 95% H2O/5% D2O, Pressure 1 atm, Temperature 298 K, pH 6.0

#	Name	Isotope labeling	Concentration
1	entity	[U-99% 13C; U-98% 15N]	1.2 mM
2	sodium phosphate	natural abundance	20 mM
3	sodium chloride	natural abundance	50 mM
4	sodium azide	natural abundance	5 mM
5	H2O	natural abundance	95 %
6	D2O	natural abundance	5 %

LACS Plot; CA

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹H	DSS	methyl protons	internal	direct	1.0
¹³C	DSS	methyl protons	na	indirect	0.2514495
¹⁵N	DSS	methyl protons	na	indirect	0.1013291

Referencing offset: 2.72 ppm, Outliers: 2 Detail

LACS Plot; CB

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹H	DSS	methyl protons	internal	direct	1.0
¹³C	DSS	methyl protons	na	indirect	0.2514495
¹⁵N	DSS	methyl protons	na	indirect	0.1013291

Referencing offset: 2.72 ppm, Outliers: 2 Detail

LACS Plot; HA

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹H	DSS	methyl protons	internal	direct	1.0
¹³C	DSS	methyl protons	na	indirect	0.2514495
¹⁵N	DSS	methyl protons	na	indirect	0.1013291

Referencing offset: 0.06 ppm, Outliers: 1 Detail

LACS Plot; CO

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹H	DSS	methyl protons	internal	direct	1.0
¹³C	DSS	methyl protons	na	indirect	0.2514495
¹⁵N	DSS	methyl protons	na	indirect	0.1013291

Referencing offset: 2.85 ppm, Outliers: 1 Detail

Protein Blocks Logo

Calculated from 20 models in PDB: 2MWM, Strand ID: A Detail

Release date

2014-12-07

Citation

NMR structure of the protein YP_193882.1 from Lactobacillus acidophilus NCFM in presence of FMN
Dutta, S.K., Serrano, P., Geralt, M., Wuthrich, K.
Not known

Related entities 1. NMR structure of the protein YP 193882.1 from Lactobacillus acidophilus NCFM in presence of FMN, entity, : 1 : 1 : 1 : 43 entities Detail

Experiments performed 7 experiments Detail

1 APSY 4D-HACANH