Solution structure of 53BP1 tandem Tudor domains in complex with a p53K370me2 peptide
GHMNSFVGLR VVAKWSSNGY FYSGKITRDV GAGKYKLLFD DGYECDVLGK DILLCDPIPL DTEVTALSED EYFSAGVVKG HRKESGELYY SIEKEGQRKW YKRMAVILSL EQGNRLREQY GLG
Polymer type: polypeptide(L)
Total | 1H | 13C | 15N | |
---|---|---|---|---|
All | 94.2 % (1508 of 1600) | 95.9 % (800 of 834) | 94.5 % (588 of 622) | 83.3 % (120 of 144) |
Backbone | 91.2 % (746 of 818) | 91.7 % (264 of 288) | 93.4 % (369 of 395) | 83.7 % (113 of 135) |
Sidechain | 97.8 % (883 of 903) | 98.2 % (536 of 546) | 97.7 % (340 of 348) | 77.8 % (7 of 9) |
Aromatic | 91.4 % (139 of 152) | 96.1 % (73 of 76) | 89.2 % (66 of 74) | 0.0 % (0 of 2) |
Methyl | 97.0 % (128 of 132) | 97.0 % (64 of 66) | 97.0 % (64 of 66) |
1. entity 1
GHMNSFVGLR VVAKWSSNGY FYSGKITRDV GAGKYKLLFD DGYECDVLGK DILLCDPIPL DTEVTALSED EYFSAGVVKG HRKESGELYY SIEKEGQRKW YKRMAVILSL EQGNRLREQY GLG2. entity 2
RAHSSHLXSK KGQSTSolvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 298 K, pH 7.5
# | Name | Isotope labeling | Type | Concentration |
---|---|---|---|---|
1 | sodium phosphate | natural abundance | 25 mM | |
2 | sodium azide | natural abundance | 1.5 mM | |
3 | 53BP1-Tudor | [U-100% 13C; U-100% 15N] | 2.0 mM | |
4 | p53K370me2 | natural abundance | 6.0 mM | |
5 | D2O | natural abundance | 10 % | |
6 | H2O | natural abundance | 90 % |
Solvent system 100% D2O, Pressure 1 atm, Temperature 298 K, pH 7.5
# | Name | Isotope labeling | Type | Concentration |
---|---|---|---|---|
7 | sodium phosphate | natural abundance | 25 mM | |
8 | sodium azide | natural abundance | 1.5 mM | |
9 | 53BP1-Tudor | [U-100% 13C; U-100% 15N] | 2.0 mM | |
10 | p53K370me2 | natural abundance | 6.0 mM | |
11 | D2O | natural abundance | 100 % |
Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 298 K, pH 7.5
# | Name | Isotope labeling | Type | Concentration |
---|---|---|---|---|
12 | sodium phosphate | natural abundance | 25 mM | |
13 | sodium azide | natural abundance | 1.5 mM | |
14 | 53BP1-Tudor | natural abundance | 5.0 mM | |
15 | p53Kc370me2 | [U-100% 13C; U-100% 15N] | 2.0 mM | |
16 | D2O | natural abundance | 10 % | |
17 | H2O | natural abundance | 90 % |
Solvent system 100% D2O, Pressure 1 atm, Temperature 298 K, pH 7.5
# | Name | Isotope labeling | Type | Concentration |
---|---|---|---|---|
18 | sodium phosphate | natural abundance | 25 mM | |
19 | sodium azide | natural abundance | 1.5 mM | |
20 | 53BP1-Tudor | natural abundance | 5.0 mM | |
21 | p53Kc370me2 | [U-100% 13C; U-100% 15N] | 2.0 mM | |
22 | D2O | natural abundance | 100 % |
Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 298 K, pH 7.5
# | Name | Isotope labeling | Type | Concentration |
---|---|---|---|---|
23 | sodium phosphate | natural abundance | 25 mM | |
24 | sodium azide | natural abundance | 1.5 mM | |
25 | 53BP1-Tudor | [U-100% 15N] | 2.0 mM | |
26 | p53K370me2 | natural abundance | 6.0 mM | |
27 | D2O | natural abundance | 10 % | |
28 | H2O | natural abundance | 90 % |
Solvent system 100% D2O, Pressure 1 atm, Temperature 298 K, pH 7.5
# | Name | Isotope labeling | Type | Concentration |
---|---|---|---|---|
29 | sodium phosphate | natural abundance | 35 mM | |
30 | sodium azide | natural abundance | 1.5 mM | |
31 | p53K370me2 | natural abundance | 2.0 mM | |
32 | D2O | natural abundance | 100 % |
Solvent system 100% D2O, Pressure 1 atm, Temperature 298 K, pH 7.5
# | Name | Isotope labeling | Type | Concentration |
---|---|---|---|---|
33 | sodium phosphate | natural abundance | 25 mM | |
34 | sodium azide | natural abundance | 1.5 mM | |
35 | p53Kc370me2 | [U-100% 13C; U-100% 15N] | 2.0 mM | |
36 | D2O | natural abundance | 10 % | |
37 | H2O | natural abundance | 90 % |