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BMRB: 25402

2MXC

Solution structure of the full length sorting nexin 3

Authors

Lenoir, M.M.L., Rajesh, S.S.R., Gruenberg, J.J.G., Overduin, M.M.O., Kaur, J.J.K.

Assembly

SNX3FL

Entity

1. SNX3FL (polymer, Thiol state: all free), 172 monomers, 19913.31 Da Detail

MAHHHHHHVG TAETVADTRR LITKPQNLND AYGPPSNFLE IDVSNPQTVG VGRGRFTTYE IRVKTNLPIF KLKESTVRRR YSDFEWLRSE LERESKVVVP PLPGKAFLRQ LPFRGDDGIF DDNFIEERKQ GLEQFINKVA GHPLAQNERC LHMFLQDEII DKSYTPSKIR HA

Formula weight

19913.31 Da

Source organism

Homo sapiens

Exptl. method

solution NMR

Refine. method

molecular dynamics

Data set

assigned_chemical_shifts

Chem. Shift Complete

Sequence coverage: 95.3 %, Completeness: 90.5 %, Completeness (bb): 93.3 % Detail

Polymer type: polypeptide(L)

	Total	¹H	¹³C	¹⁵N
All	90.5 % (1873 of 2069)	91.1 % (990 of 1087)	89.2 % (718 of 805)	93.2 % (165 of 177)
Backbone	93.3 % (942 of 1010)	93.3 % (320 of 343)	93.3 % (472 of 506)	93.2 % (150 of 161)
Sidechain	88.7 % (1083 of 1221)	90.1 % (670 of 744)	86.3 % (398 of 461)	93.8 % (15 of 16)
Aromatic	61.7 % (111 of 180)	74.4 % (67 of 90)	48.3 % (43 of 89)	100.0 % (1 of 1)
Methyl	98.9 % (174 of 176)	98.9 % (87 of 88)	98.9 % (87 of 88)

1. SNX3FL

MAHHHHHHVG TAETVADTRR LITKPQNLND AYGPPSNFLE IDVSNPQTVG VGRGRFTTYE IRVKTNLPIF KLKESTVRRR YSDFEWLRSE LERESKVVVP PLPGKAFLRQ LPFRGDDGIF DDNFIEERKQ GLEQFINKVA GHPLAQNERC LHMFLQDEII DKSYTPSKIR HA

Show sample condition

Sample

Solvent system 90% H2O/10% D2O, Pressure 1.000 atm, Temperature 298 K, pH 6.5

#	Name	Isotope labeling	Concentration
1	SNX3FL	[U-100% 13C; U-100% 15N]	0.5 mM
2	NaCl	natural abundance	100 mM
3	Sodium phosphate	natural abundance	20 mM

LACS Plot; CA

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
¹³C	HDO	H	4.763 ppm	internal	indirect	0.2514495
¹H	HDO	H	4.763 ppm	internal	indirect	1.0
¹⁵N	HDO	H	4.763 ppm	internal	indirect	0.1013291

Referencing offset: -0.1 ppm, Outliers: 1 Detail

LACS Plot; CB

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
¹³C	HDO	H	4.763 ppm	internal	indirect	0.2514495
¹H	HDO	H	4.763 ppm	internal	indirect	1.0
¹⁵N	HDO	H	4.763 ppm	internal	indirect	0.1013291

Referencing offset: -0.1 ppm, Outliers: 1 Detail

LACS Plot; HA

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
¹³C	HDO	H	4.763 ppm	internal	indirect	0.2514495
¹H	HDO	H	4.763 ppm	internal	indirect	1.0
¹⁵N	HDO	H	4.763 ppm	internal	indirect	0.1013291

Referencing offset: 0.06 ppm, Outliers: 6 Detail

LACS Plot; CO

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
¹³C	HDO	H	4.763 ppm	internal	indirect	0.2514495
¹H	HDO	H	4.763 ppm	internal	indirect	1.0
¹⁵N	HDO	H	4.763 ppm	internal	indirect	0.1013291

Referencing offset: -0.07 ppm, Outliers: 2 Detail

Protein Blocks Logo

Calculated from 20 models in PDB: 2MXC, Strand ID: A Detail

Release date

2015-08-12

Citation

Secondary structure and (1)H, (13)C, (15)N resonance assignments of the endosomal sorting protein sorting nexin 3
Overduin, M., Rajesh, S., Gruenberg, J., Lenoir, M.
Biomol. NMR Assign. (2015), 9, 355-358, PubMed 25893673 , DOI 10.1007/s12104-015-9609-z , Abstract

Related entities 1. SNX3FL, : 4 : 25 entities Detail

Interaction partners 1. SNX3FL, : 27 interactors Detail

More details for 27 interactors identified by 11 citations

Experiments performed 12 experiments Detail

1 2D 1H-15N HSQC/HMQC