BMRB: 25415

Structural features of a 3' splice site influenza A: 19-nt duplex

Authors

Turner, D.H., Kennedy, S.D., Chen, J.L.

Assembly

3' splice site influenza A: 19-nt duplex

Entity

1. 3' splice site influenza A: 19-nt duplex (polymer, Thiol state: not present), 19 monomers, 6234.724 Da Detail

GCAGGCCCAU GGGAGUGCA

Formula weight

6234.724 Da

Exptl. method

solution NMR

Data set

assigned_chemical_shifts

Chem. Shift Complete

Sequence coverage: 100.0 %, Completeness: 83.6 %, Completeness (bb): 79.7 % Detail

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Polymer type: polyribonucleotide

	Total	1H	31P
All	83.6 % (153 of 183)	84.8 % (139 of 164)	73.7 % (14 of 19)
Suger, PO4	79.7 % (106 of 133)	80.7 % (92 of 114)	73.7 % (14 of 19)
Nucleobase	94.0 % (47 of 50)	94.0 % (47 of 50)
Aromatic	92.5 % (37 of 40)	92.5 % (37 of 40)

1. RNA (19-MER)

GCAGGCCCAU GGGAGUGCA

Show sample condition

Sample #1

Solvent system 95% H2O/5% D2O, Pressure 1 atm, Temperature 298.15 K, pH 6.0, Details 19-nt duplex

#	Name	Isotope labeling	Type	Concentration
1	entity	natural abundance		4.0 mM
2	H2O	natural abundance		95 %
3	D2O	natural abundance		5 %

Sample #2

Solvent system 100% D2O, Pressure 1 atm, Temperature 298.15 K, pH 6.0, Details 19-nt duplex

#	Name	Isotope labeling	Type	Concentration
4	entity	natural abundance		1.0 mM
5	D2O	natural abundance		100 %

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Release date

2015-05-03

Citation

Structural features of a 3' splice site in influenza a
Chen, J.L., Kennedy, S.D., Turner, D.H.
Biochemistry (2015), 54, 3269-3285, PubMed 25909229 , DOI 10.1021/acs.biochem.5b00012 , Abstract

Experiments performed 7 experiments Detail