NMR structure of spider toxin- G7W/N24S mutant of TRTX-Hhn2b
ID | Type | Value order | Atom ID 1 | Atom ID 2 |
---|---|---|---|---|
1 | disulfide | sing | 1:CYS3:SG | 1:CYS18:SG |
2 | disulfide | sing | 1:CYS10:SG | 1:CYS23:SG |
3 | disulfide | sing | 1:CYS17:SG | 1:CYS30:SG |
Polymer type: polypeptide(L)
Total | 1H | 13C | 15N | |
---|---|---|---|---|
All | 98.2 % (382 of 389) | 99.0 % (202 of 204) | 97.3 % (145 of 149) | 97.2 % (35 of 36) |
Backbone | 96.5 % (195 of 202) | 97.1 % (68 of 70) | 96.0 % (95 of 99) | 97.0 % (32 of 33) |
Sidechain | 100.0 % (218 of 218) | 100.0 % (134 of 134) | 100.0 % (81 of 81) | 100.0 % (3 of 3) |
Aromatic | 100.0 % (42 of 42) | 100.0 % (21 of 21) | 100.0 % (19 of 19) | 100.0 % (2 of 2) |
Methyl | 100.0 % (20 of 20) | 100.0 % (10 of 10) | 100.0 % (10 of 10) |
1. GWHhn2b
AECKGFWKSC VPGKNECCSG YACSSRDKWC KVLLSolvent system 95% H2O/5% D2O, Pressure 1 atm, Temperature 298 K, pH 5
# | Name | Isotope labeling | Type | Concentration |
---|---|---|---|---|
1 | GWHhn2b | [U-99% 13C; U-99% 15N] | 300 uM | |
2 | Sodium acetate | natural abundance | 20 mM | |
3 | D2O | natural abundance | 5 % | |
4 | H2O | natural abundance | 95 % |