BMRBj - BMREntryMaster

	Total	¹H	¹³C	¹⁵N
All	84.6 % (925 of 1094)	89.1 % (513 of 576)	76.4 % (321 of 420)	92.9 % (91 of 98)
Backbone	82.4 % (445 of 540)	94.1 % (174 of 185)	70.6 % (190 of 269)	94.2 % (81 of 86)
Sidechain	87.6 % (560 of 639)	86.7 % (339 of 391)	89.4 % (211 of 236)	83.3 % (10 of 12)
Aromatic	94.4 % (136 of 144)	94.4 % (68 of 72)	94.4 % (67 of 71)	100.0 % (1 of 1)
Methyl	100.0 % (44 of 44)	100.0 % (22 of 22)	100.0 % (22 of 22)

1. OCRE

GHMQESYSQY PVPDVSTYQY DETSGYYYDP QTGLYYDPNS QYYYNAQSQQ YLYWDGERRT YVPALEQSAD GHKETGAPSK EGKEKKEKHK TK

Show sample condition

Sample

Solvent system 90% H2O, 10% D2O, Pressure 1 atm, Temperature 308 K, pH 6.0, Details [U-98% 13C; U-98% 15N], 1.2 mM;20 mM phosphate buffer; 50 mM NaCl; 5 mM Sodium Azide; pH 6.0; 90% H2O; 10% D2O

#	Name	Isotope labeling	Concentration
1	entity	[U-98% 13C; U-98% 15N]	1.2 mM
2	sodium chloride	natural abundance	50 mM
3	sodium phosphate	natural abundance	20 mM
4	sodium azide	natural abundance	5 mM

LACS Plot; CA

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹³C	DSS	methyl protons	na	indirect	0.2514495
¹H	DSS	methyl protons	internal	direct	1.0
¹⁵N	DSS	methyl protons	na	indirect	0.1013291

Referencing offset: -0.37 ppm, Outliers: 3 Detail

LACS Plot; CB

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹³C	DSS	methyl protons	na	indirect	0.2514495
¹H	DSS	methyl protons	internal	direct	1.0
¹⁵N	DSS	methyl protons	na	indirect	0.1013291

Referencing offset: -0.37 ppm, Outliers: 3 Detail

LACS Plot; HA

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹³C	DSS	methyl protons	na	indirect	0.2514495
¹H	DSS	methyl protons	internal	direct	1.0
¹⁵N	DSS	methyl protons	na	indirect	0.1013291

Referencing offset: -0.03 ppm, Outliers: 1 Detail

Protein Blocks Logo

Calculated from 20 models in PDB: 2MXW, Strand ID: A Detail

Release date

2016-08-29

Citation

Nuclear Magnetic Resonance Structure of a Novel Globular Domain in RBM10 Containing OCRE, the Octamer Repeat Sequence Motif
Martin, B.T., Serrano, P., Geralt, M., Wuthrich, K.
Structure (2016), 24, 158-164, PubMed 26712279 , DOI 10.1016/j.str.2015.10.029 , Abstract

Related entities 1. OCRE, : 1 : 2 : 6 entities Detail

Interaction partners 1. OCRE, : 90 interactors Detail

More details for 90 interactors identified by 24 citations

Experiments performed 7 experiments Detail

1 2D 1H-15N HSQC

Instrument

Bruker Avance - 800 MHz

Sample

State isotropic, Solvent system 90% H2O, 10% D2O, Pressure 1 atm, Temperature 308 K, pH 6.0, Details [U-98% 13C; U-98% 15N], 1.2 mM;20 mM phosphate buffer; 50 mM NaCl; 5 mM Sodium Azide; pH 6.0; 90% H2O; 10% D2O

#	Name	Isotope labeling	Concentration
1	entity	[U-98% 13C; U-98% 15N]	1.2 mM
2	sodium chloride	natural abundance	50 mM
3	sodium phosphate	natural abundance	20 mM
4	sodium azide	natural abundance	5 mM

2 3D 1H-13C NOESY aliphatic

Instrument

Bruker Avance - 800 MHz

Sample

#	Name	Isotope labeling	Concentration
1	entity	[U-98% 13C; U-98% 15N]	1.2 mM
2	sodium chloride	natural abundance	50 mM
3	sodium phosphate	natural abundance	20 mM
4	sodium azide	natural abundance	5 mM

3 3D 1H-13C NOESY aromatic

Instrument

Bruker Avance - 800 MHz

Sample

#	Name	Isotope labeling	Concentration
1	entity	[U-98% 13C; U-98% 15N]	1.2 mM
2	sodium chloride	natural abundance	50 mM
3	sodium phosphate	natural abundance	20 mM
4	sodium azide	natural abundance	5 mM

4 3D 1H-15N NOESY

Instrument

Bruker Avance - 800 MHz

Sample

#	Name	Isotope labeling	Concentration
1	entity	[U-98% 13C; U-98% 15N]	1.2 mM
2	sodium chloride	natural abundance	50 mM
3	sodium phosphate	natural abundance	20 mM
4	sodium azide	natural abundance	5 mM

5 APSY 4D-HACANH

Instrument

Bruker Avance - 600 MHz

Sample

#	Name	Isotope labeling	Concentration
1	entity	[U-98% 13C; U-98% 15N]	1.2 mM
2	sodium chloride	natural abundance	50 mM
3	sodium phosphate	natural abundance	20 mM
4	sodium azide	natural abundance	5 mM

6 APSY 5D-CBCACONH

Instrument

Bruker Avance - 600 MHz

Sample

#	Name	Isotope labeling	Concentration
1	entity	[U-98% 13C; U-98% 15N]	1.2 mM
2	sodium chloride	natural abundance	50 mM
3	sodium phosphate	natural abundance	20 mM
4	sodium azide	natural abundance	5 mM

7 APSY 5D-HACACONH

Instrument

Bruker Avance - 600 MHz

Sample

#	Name	Isotope labeling	Concentration
1	entity	[U-98% 13C; U-98% 15N]	1.2 mM
2	sodium chloride	natural abundance	50 mM
3	sodium phosphate	natural abundance	20 mM
4	sodium azide	natural abundance	5 mM

NMR combined restraints 3 contents Detail

Properties

Input source #1: NMR data (NEF) - Assigned chemical shifts, Distance restraints	combined_25434_2mxw.nef
Input source #2: Coordindates	2mxw.cif
Diamagnetism of the molecular assembly	True (excluding Oxygen atoms)
Whether the assembly has a disulfide bond	None
Whether the assembly has a other bond	None
Whether the assembly contains a cyclic polymer	None
Overall data processing status	Warning

Disulfide bonds

None

Other bonds (neither disulfide, covalent nor hydrogen bonds, e.g. Zinc–sulphur bond)

None

Non-standard residues

None

Sequence alignments

Entity_assembly_ID (NMR): A vs Auth_asym_ID (model): A

--------10--------20--------30--------40--------50--------60--------70--------80--------90--
GHMQESYSQYPVPDVSTYQYDETSGYYYDPQTGLYYDPNSQYYYNAQSQQYLYWDGERRTYVPALEQSADGHKETGAPSKEGKEKKEKHKTK
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
GHMQESYSQYPVPDVSTYQYDETSGYYYDPQTGLYYDPNSQYYYNAQSQQYLYWDGERRTYVPALEQSADGHKETGAPSKEGKEKKEKHKTK

Chain assignments

Entity_assembly_ID (NMR)	Auth_asym_ID (model)	Length	Unmapped	Conflict	Sequence coverage (%)
A	A	92	0	0	100.0

Content subtype: combined_25434_2mxw.nef

#	Content subtype	Saveframe	Status	# of rows (sets)	Experiment type	Sequence coverage (%)
1	Assigned chemical shifts	assigned_chem_shift_list_1	Warning	892		94.6 (chain: A, length: 92)
1	Distance restraints	DYANA/DIANA_distance_constraints_2	OK	1454 (1)	noe	94.6 (chain: A, length: 92)

Assigned chemical shifts

Saveframe: assigned_chem_shift_list_1
- Status: Warning
- Sequence coverage of experimental data
  - Entity_assembly_ID: A, Chain length: 92, Sequence coverage: 94.6%
- Total number of assignments
  - ¹H chemical shifts: 505
  - ¹³C chemical shifts: 294
  - ¹⁵N chemical shifts: 93
- Completeness of assignments
  - Entity_assemble_ID: A
- Peptide bond of PRO
- Tautomeric state of HIS
- Rotameric state of ILE , LEU, and VAL
- Histogram of normalized assigned chemical shifts
- Random coil index and derived parameters (S² and NMR RMSD)
  - Chain_ID: A

Comp_index_ID	Comp_ID	Atom_ID	CS value (ppm)
59	ARG	HH22	10.188
59	ARG	NH2	73.2

Atom group	# of target shifts	# of assigned shifts	Completeness (%)
¹H chemical shifts	576	504	87.5
¹³C chemical shifts	420	294	70.0
¹⁵N chemical shifts	100	92	92.0

Atom group	# of target shifts	# of assigned shifts	Completeness (%)
¹H chemical shifts	185	173	93.5
¹³C chemical shifts	184	87	47.3
¹⁵N chemical shifts	86	80	93.0

Atom group	# of target shifts	# of assigned shifts	Completeness (%)
¹H chemical shifts	391	331	84.7
¹³C chemical shifts	236	207	87.7
¹⁵N chemical shifts	14	12	85.7

Atom group	# of target shifts	# of assigned shifts	Completeness (%)
¹H chemical shifts	23	22	95.7
¹³C chemical shifts	23	22	95.7

Atom group	# of target shifts	# of assigned shifts	Completeness (%)
¹H chemical shifts	72	68	94.4
¹³C chemical shifts	71	67	94.4
¹⁵N chemical shifts	1	1	100.0

Distance restraints

Saveframe: DYANA/DIANA_distance_constraints_2
- Status: OK
- Experiment type: noe
- Sequence coverage of experimental data
  - Entity_assembly_ID: A, Chain length: 92, Sequence coverage: 94.6%
- Total number of restraints: 1454
- Weight of restraints: 1.0 (1454)
- Potential type of restraints: upper-bound-parabolic (1454)
- Range of distance target values: 2.4, 5.5 (Å)
- Histogram of distance target values
- Histogram of discrepancy in distance target values
- Distance restraints per residue
  - Chain_ID: A
- Distance restraints on contact map

Keywords Unknown Function

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Found 1 Document (3.406 seconds)

BMRB: 25434

Sample

Chemical shift reference

Chemical shift reference

Chemical shift reference

Related entities 1. OCRE, : 1 : 2 : 6 entities Detail

Interaction partners 1. OCRE, : 90 interactors Detail

Experiments performed 7 experiments Detail

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NMR combined restraints 3 contents Detail