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Found 1 Document (0.843 seconds)

BMRB: 25436

2MXY

Solution structure of hnRNP C RRM in complex with the 5'-AUUUUUC-3' RNA

Authors

Cienikova, Z., Damberger, F.F., Hall, J., Allain, F.H-T., Maris, C.

Assembly

hnRNP C RRM in complex with the 5'-AUUUUUC-3' RNA

Entity

1. hnRNP C (polymer, Thiol state: all free), 105 monomers, 11274.78 Da Detail

ASNVTNKTDP RSMNSRVFIG NLNTLVVKKS DVEAIFSKYG KIVGCSVHKG FAFVQYVNER NARAAVAGED GRMIAGQVLD INLAAEPKVN RGKAGVKRSA AEMYG

2. RNA (5'-R(*AP*UP*UP*UP*UP*UP*C)-3') (polymer, Thiol state: not present), 7 monomers, 2183.231 Da Detail

AUUUUUC

Total weight

13458.012 Da

Max. entity weight

11274.78 Da

Source organism

Homo sapiens

Exptl. method

solution NMR

Refine. method

simulated annealing, torsion angle dynamics

Data set

assigned_chemical_shifts

Chem. Shift Complete

Sequence coverage: 100.0 %, Completeness: 96.4 %, Completeness (bb): 99.1 % Detail

Polymer type: polypeptide(L) polyribonucleotide

	Total	¹H	¹³C	¹⁵N
All	96.4 % (1249 of 1295)	98.8 % (666 of 674)	93.4 % (469 of 502)	95.8 % (114 of 119)
Backbone	99.1 % (697 of 703)	100.0 % (260 of 260)	98.2 % (334 of 340)	100.0 % (103 of 103)
Sidechain	94.2 % (647 of 687)	98.1 % (406 of 414)	89.5 % (230 of 257)	68.8 % (11 of 16)
Aromatic	66.0 % (70 of 106)	92.5 % (49 of 53)	43.8 % (21 of 48)	0.0 % (0 of 5)
Methyl	100.0 % (120 of 120)	100.0 % (60 of 60)	100.0 % (60 of 60)

1. hnRNP C

ASNVTNKTDP RSMNSRVFIG NLNTLVVKKS DVEAIFSKYG KIVGCSVHKG FAFVQYVNER NARAAVAGED GRMIAGQVLD INLAAEPKVN RGKAGVKRSA AEMYG

2. RNA (5'-R(*AP*UP*UP*UP*UP*UP*C)-3')

AUUUUUC

Show sample condition

Sample #1

Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 293 K, pH 6.5

#	Name	Isotope labeling	Concentration
1	hnRNP C	[U-100% 15N]	1.0 mM
2	RNA (5'-R(APUPUPUPUPUP*C)-3')	natural abundance	1.0 mM
3	sodium phosphate	natural abundance	20 mM
4	sodium chloride	natural abundance	10 mM
5	H2O	natural abundance	90 %
6	D2O	natural abundance	10 %

Sample #2

Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 293 K, pH 6.5

#	Name	Isotope labeling	Concentration
7	hnRNP C	[U-100% 13C; U-100% 15N]	1.0 mM
8	RNA (5'-R(APUPUPUPUPUP*C)-3')	natural abundance	1.0 mM
9	sodium phosphate	natural abundance	20 mM
10	sodium chloride	natural abundance	10 mM
11	H2O	natural abundance	90 %
12	D2O	natural abundance	10 %

Sample #3

Solvent system 100% D2O, Pressure 1 atm, Temperature 293 K, pH 6.5

#	Name	Isotope labeling	Concentration
13	hnRNP C	[U-100% 13C; U-100% 15N]	1.0 mM
14	RNA (5'-R(APUPUPUPUPUP*C)-3')	natural abundance	1.0 mM
15	sodium phosphate	natural abundance	20 mM
16	sodium chloride	natural abundance	10 mM
17	D2O	natural abundance	100 %

Sample #4

Solvent system 100% D2O, Pressure 1 atm, Temperature 293 K, pH 6.5

#	Name	Isotope labeling	Concentration
18	hnRNP C	[U-100% 15N]	0.5 ~ 1.0 mM
19	RNA (5'-R(APUPUPUPUPUP*C)-3')	[100% 13C5]-Ura	0.5 ~ 1.0 mM
20	sodium phosphate	natural abundance	20 mM
21	sodium chloride	natural abundance	10 mM
22	D2O	natural abundance	100 %

LACS Plot; CA

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹³C	DSS	methyl protons	external	indirect	0.2514495
¹H	DSS	methyl protons	external	direct	1.0
¹⁵N	DSS	methyl protons	external	indirect	0.1013291

Referencing offset: -0.13 ppm, Outliers: 2 Detail

LACS Plot; CB

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹³C	DSS	methyl protons	external	indirect	0.2514495
¹H	DSS	methyl protons	external	direct	1.0
¹⁵N	DSS	methyl protons	external	indirect	0.1013291

Referencing offset: -0.13 ppm, Outliers: 2 Detail

LACS Plot; HA

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹³C	DSS	methyl protons	external	indirect	0.2514495
¹H	DSS	methyl protons	external	direct	1.0
¹⁵N	DSS	methyl protons	external	indirect	0.1013291

Referencing offset: 0.01 ppm, Outliers: 1 Detail

LACS Plot; CO

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹³C	DSS	methyl protons	external	indirect	0.2514495
¹H	DSS	methyl protons	external	direct	1.0
¹⁵N	DSS	methyl protons	external	indirect	0.1013291

Referencing offset: 0.31 ppm, Outliers: 3 Detail

Protein Blocks Logo

Calculated from 20 models in PDB: 2MXY, Strand ID: A Detail

Release date

2015-04-13

Citation

Structural and mechanistic insights into poly(uridine) tract recognition by the hnRNP C RNA recognition motif
Cienikova, Z., Damberger, F.F., Hall, J., Allain, F.H-T., Maris, C.
J. Am. Chem. Soc. (2014), 136, 14536-14544, PubMed 25216038 , DOI 10.1021/ja507690d , Abstract

Related entities 1. hnRNP C, : 1 : 8 : 60 entities Detail

Experiments performed 10 experiments Detail

1 2D 1H-15N HSQC

Instrument

Bruker Avance - 900 MHz with cryoprobe

Sample

State isotropic, Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 293 K, pH 6.5

#	Name	Isotope labeling	Concentration
1	hnRNP C	[U-100% 15N]	1.0 mM
2	RNA (5'-R(APUPUPUPUPUP*C)-3')	natural abundance	1.0 mM
3	sodium phosphate	natural abundance	20 mM
4	sodium chloride	natural abundance	10 mM
5	H2O	natural abundance	90 %
6	D2O	natural abundance	10 %

2 3D HNCACB

Instrument

Bruker AVIII - 700 MHz with cryoprobe

Sample

State isotropic, Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 293 K, pH 6.5

#	Name	Isotope labeling	Concentration
7	hnRNP C	[U-100% 13C; U-100% 15N]	1.0 mM
8	RNA (5'-R(APUPUPUPUPUP*C)-3')	natural abundance	1.0 mM
9	sodium phosphate	natural abundance	20 mM
10	sodium chloride	natural abundance	10 mM
11	H2O	natural abundance	90 %
12	D2O	natural abundance	10 %

3 3D HNCO

Instrument

Bruker AVIII - 750 MHz

Sample

State isotropic, Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 293 K, pH 6.5

#	Name	Isotope labeling	Concentration
7	hnRNP C	[U-100% 13C; U-100% 15N]	1.0 mM
8	RNA (5'-R(APUPUPUPUPUP*C)-3')	natural abundance	1.0 mM
9	sodium phosphate	natural abundance	20 mM
10	sodium chloride	natural abundance	10 mM
11	H2O	natural abundance	90 %
12	D2O	natural abundance	10 %

4 3D 1H-15N NOESY

Instrument

Bruker Avance - 900 MHz with cryoprobe

Sample

State isotropic, Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 293 K, pH 6.5

#	Name	Isotope labeling	Concentration
1	hnRNP C	[U-100% 15N]	1.0 mM
2	RNA (5'-R(APUPUPUPUPUP*C)-3')	natural abundance	1.0 mM
3	sodium phosphate	natural abundance	20 mM
4	sodium chloride	natural abundance	10 mM
5	H2O	natural abundance	90 %
6	D2O	natural abundance	10 %

5 3D HCCH-TOCSY

Instrument

Bruker AVIII - 600 MHz with cryoprobe

Sample

State isotropic, Solvent system 100% D2O, Pressure 1 atm, Temperature 293 K, pH 6.5

#	Name	Isotope labeling	Concentration
13	hnRNP C	[U-100% 13C; U-100% 15N]	1.0 mM
14	RNA (5'-R(APUPUPUPUPUP*C)-3')	natural abundance	1.0 mM
15	sodium phosphate	natural abundance	20 mM
16	sodium chloride	natural abundance	10 mM
17	D2O	natural abundance	100 %

6 3D 1H-13C NOESY aliphatic

Instrument

Bruker AVIII - 700 MHz with cryoprobe

Sample

State isotropic, Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 293 K, pH 6.5

#	Name	Isotope labeling	Concentration
7	hnRNP C	[U-100% 13C; U-100% 15N]	1.0 mM
8	RNA (5'-R(APUPUPUPUPUP*C)-3')	natural abundance	1.0 mM
9	sodium phosphate	natural abundance	20 mM
10	sodium chloride	natural abundance	10 mM
11	H2O	natural abundance	90 %
12	D2O	natural abundance	10 %

7 3D 1H-13C edited-filtered NOESY

Instrument

Bruker AVIII - 600 MHz with cryoprobe

Sample

State isotropic, Solvent system 100% D2O, Pressure 1 atm, Temperature 293 K, pH 6.5

#	Name	Isotope labeling	Concentration
18	hnRNP C	[U-100% 15N]	0.5 ~ 1.0 mM
19	RNA (5'-R(APUPUPUPUPUP*C)-3')	[100% 13C5]-Ura	0.5 ~ 1.0 mM
20	sodium phosphate	natural abundance	20 mM
21	sodium chloride	natural abundance	10 mM
22	D2O	natural abundance	100 %

8 3D 1H-13C edited-filtered NOESY

Instrument

Bruker AVIII - 500 MHz with cryoprobe

Sample

State isotropic, Solvent system 100% D2O, Pressure 1 atm, Temperature 293 K, pH 6.5

#	Name	Isotope labeling	Concentration
13	hnRNP C	[U-100% 13C; U-100% 15N]	1.0 mM
14	RNA (5'-R(APUPUPUPUPUP*C)-3')	natural abundance	1.0 mM
15	sodium phosphate	natural abundance	20 mM
16	sodium chloride	natural abundance	10 mM
17	D2O	natural abundance	100 %

9 2D 1H-13C HSQC

Instrument

Bruker Avance - 900 MHz with cryoprobe

Sample

State isotropic, Solvent system 100% D2O, Pressure 1 atm, Temperature 293 K, pH 6.5

#	Name	Isotope labeling	Concentration
18	hnRNP C	[U-100% 15N]	0.5 ~ 1.0 mM
19	RNA (5'-R(APUPUPUPUPUP*C)-3')	[100% 13C5]-Ura	0.5 ~ 1.0 mM
20	sodium phosphate	natural abundance	20 mM
21	sodium chloride	natural abundance	10 mM
22	D2O	natural abundance	100 %

10 2D 1H-1H NOESY

Instrument

Bruker Avance - 900 MHz with cryoprobe

Sample

State isotropic, Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 293 K, pH 6.5

#	Name	Isotope labeling	Concentration
1	hnRNP C	[U-100% 15N]	1.0 mM
2	RNA (5'-R(APUPUPUPUPUP*C)-3')	natural abundance	1.0 mM
3	sodium phosphate	natural abundance	20 mM
4	sodium chloride	natural abundance	10 mM
5	H2O	natural abundance	90 %
6	D2O	natural abundance	10 %

nullKeywords RNA, RRM, complex, hnRNP C

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Found 1 Document (0.843 seconds)

BMRB: 25436

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Chemical shift reference

Chemical shift reference

Chemical shift reference

Chemical shift reference

Related entities 1. hnRNP C, : 1 : 8 : 60 entities Detail

Experiments performed 10 experiments Detail

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