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BMRB: 25437

2MXZ

Endo T5-ZN+2

Authors

Prokhorov, D.A., Mikoulinskaia, G.V., Molochkov, N.V., Kutyshenko, V.P.

Assembly

Endo T5-ZN+2

Entity

1. l-alanoyl-d-glutamate peptidase (polymer, Thiol state: not present), 137 monomers, 15264.02 Da Detail

MSFKFGKNSE KQLATVKPEL QKVARRALEL SPYDFTIVQG IRTVAQSAQN IANGTSFLKD PSKSKHITGD AIDFAPYING KIDWNDLEAF WAVKKAFEQA GKELGIKLRF GADWNASGDY HDEIKRGTYD GGHVELV

2. ZINC ION (non-polymer), 65.409 Da

Total weight

15329.429 Da

Max. entity weight

15264.02 Da

Source organism

Escherichia virus T5

Exptl. method

solution NMR

Refine. method

simulated annealing

Data set

assigned_chemical_shifts

Chem. Shift Complete

Sequence coverage: 97.8 %, Completeness: 90.1 %, Completeness (bb): 84.6 % Detail

Polymer type: polypeptide(L)

	Total	¹H	¹³C	¹⁵N
All	90.1 % (1454 of 1613)	96.1 % (806 of 839)	81.8 % (512 of 626)	91.9 % (136 of 148)
Backbone	84.6 % (689 of 814)	96.5 % (272 of 282)	72.4 % (289 of 399)	96.2 % (128 of 133)
Sidechain	96.1 % (888 of 924)	95.9 % (534 of 557)	98.3 % (346 of 352)	53.3 % (8 of 15)
Aromatic	100.0 % (160 of 160)	100.0 % (80 of 80)	100.0 % (77 of 77)	100.0 % (3 of 3)
Methyl	100.0 % (140 of 140)	100.0 % (70 of 70)	100.0 % (70 of 70)

1. l-alanoyl-d-glutamate peptidase

MSFKFGKNSE KQLATVKPEL QKVARRALEL SPYDFTIVQG IRTVAQSAQN IANGTSFLKD PSKSKHITGD AIDFAPYING KIDWNDLEAF WAVKKAFEQA GKELGIKLRF GADWNASGDY HDEIKRGTYD GGHVELV

Show sample condition

Sample

Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 298 K, pH 4.1

#	Name	Isotope labeling	Concentration
1	l-alanoyl-d-glutamate peptidase	[U-100% 13C; U-100% 15N]	0.8 mM
2	H2O	natural abundance	90 %
3	D2O	natural abundance	10 %

LACS Plot; CA

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹³C	DSS	methyl protons	na	indirect	0.2514495
¹H	DSS	methyl protons	internal	direct	1.0
¹⁵N	DSS	methyl protons	na	indirect	0.1013291

Referencing offset: -0.16 ppm, Outliers: 2 Detail

LACS Plot; CB

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹³C	DSS	methyl protons	na	indirect	0.2514495
¹H	DSS	methyl protons	internal	direct	1.0
¹⁵N	DSS	methyl protons	na	indirect	0.1013291

Referencing offset: -0.16 ppm, Outliers: 2 Detail

LACS Plot; HA

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹³C	DSS	methyl protons	na	indirect	0.2514495
¹H	DSS	methyl protons	internal	direct	1.0
¹⁵N	DSS	methyl protons	na	indirect	0.1013291

Referencing offset: 0.03 ppm, Outliers: 2 Detail

Protein Blocks Logo

Calculated from 20 models in PDB: 2MXZ, Strand ID: A Detail

Release date

2016-08-29

Citation

Structure and dynamics of the retro-form of the bacteriophage T5 endolysin
Kutyshenko, V.P., Mikoulinskaia, G.V., Molochkov, N.V., Prokhorov, D.A., Taran, S.A., Uversky, V.N.
Biochim. Biophys. Acta (2016), 1864, 1281-1291, PubMed 27376687 , DOI 10.1016/j.bbapap.2016.06.018 , Abstract

Related entities 1. l-alanoyl-d-glutamate peptidase, : 1 : 2 : 2 entities Detail

Experiments performed 10 experiments Detail

1 2D 1H-15N HSQC