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BMRB: 25442

2MY2

Snu17p-Bud13p structure intermediate during RES complex assembly

Authors

Wysoczanski, P., Becker, S., Zweckstetter, M.

Assembly

Heterodimeric complex composed of Snu17p/Ist3p(25-138) and Bud13p(215-255

Entity

1. Snu17p (polymer, Thiol state: not present), 118 monomers, 13478.84 Da Detail

GAMGNEYKDN AYIYIGNLNR ELTEGDILTV FSEYGVPVDV ILSRDENTGE SQGFAYLKYE DQRSTILAVD NLNGFKIGGR ALKIDHTFYR PKRSLQKYYE AVKEELDRDI VSKNNAEK

2. Bud13p (polymer, Thiol state: not present), 41 monomers, 4880.326 Da Detail

YDKPAPENRF AIMPGSRWDG VHRSNGFEEK WFAKQNEINE K

Total weight

18359.166 Da

Max. entity weight

13478.84 Da

Source organism

Saccharomyces cerevisiae

Exptl. method

solution NMR

Refine. method

simulated annealing

Data set

assigned_chemical_shifts

Chem. Shift Complete

Sequence coverage: 98.1 %, Completeness: 82.6 %, Completeness (bb): 81.6 % Detail

Polymer type: polypeptide(L)

	Total	¹H	¹³C	¹⁵N
All	82.6 % (1565 of 1895)	87.6 % (872 of 995)	74.8 % (544 of 727)	86.1 % (149 of 173)
Backbone	81.6 % (770 of 944)	92.6 % (302 of 326)	71.1 % (330 of 464)	89.6 % (138 of 154)
Sidechain	84.4 % (926 of 1097)	85.2 % (570 of 669)	84.4 % (345 of 409)	57.9 % (11 of 19)
Aromatic	77.5 % (141 of 182)	82.4 % (75 of 91)	71.9 % (64 of 89)	100.0 % (2 of 2)
Methyl	100.0 % (142 of 142)	100.0 % (71 of 71)	100.0 % (71 of 71)

1. Snu17p

GAMGNEYKDN AYIYIGNLNR ELTEGDILTV FSEYGVPVDV ILSRDENTGE SQGFAYLKYE DQRSTILAVD NLNGFKIGGR ALKIDHTFYR PKRSLQKYYE AVKEELDRDI VSKNNAEK

2. Bud13p

YDKPAPENRF AIMPGSRWDG VHRSNGFEEK WFAKQNEINE K

Show sample condition

Sample #1

Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 308 K, pH 6.8, Details 160-180 uL, 3 mm tube

#	Name	Isotope labeling	Concentration
1	sodium phosphate	natural abundance	25 mM
2	sodium chloride	natural abundance	250 mM
3	sodium azide	natural abundance	1 mM
4	entity_1	[U-13C; U-15N]	0.8 ~ 1.0 mM
5	entity_2	natural abundance	1.0 ~ 1.2 mM
6	H2O	natural abundance	90 %
7	D2O	natural abundance	10 %

Sample #2

Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 308 K, pH 6.8, Details 160-180 uL, 3 mm tube

#	Name	Isotope labeling	Concentration
8	sodium phosphate	natural abundance	25 mM
9	sodium chloride	natural abundance	250 mM
10	sodium azide	natural abundance	1 mM
11	entity_1	natural abundance	1.0 ~ 1.2 mM
12	entity_2	[U-13C; U-15N]	0.8 ~ 1.0 mM
13	Snu17p	natural abundance	90 %
14	Snu17p	natural abundance	10 %

Sample #3

Solvent system 90% H2O/10% D2O, Pressure 1 atm, Temperature 308 K, pH 6.8, Details 160-180 uL, 3 mm tube

#	Name	Isotope labeling	Concentration
15	sodium phosphate	natural abundance	25 mM
16	sodium chloride	natural abundance	250 mM
17	sodium azide	natural abundance	1 mM
18	entity_1	[U-13C; U-15N]	1 mM
19	entity_2	[U-13C; U-15N]	1 mM
20	H2O	natural abundance	90 %
21	D2O	natural abundance	10 %

Protein Blocks Logo

Calculated from 20 models in PDB: 2MY2, Strand ID: A, B Detail

Release date

2015-08-09

Citation

Structures of intermediates during RES complex assembly
Wysoczanski, P., Becker, S., Zweckstetter, M.
Sci. Rep. (2015), 5, 12545-12545, PubMed 26212312 , DOI 10.1038/srep12545 , Abstract

Related entities 1. Snu17p, : 15 entities Detail

Related entities 2. Bud13p, : 1 : 4 : 1 : 25 entities Detail

Interaction partners 2. Bud13p, : 19 interactors Detail

More details for 19 interactors identified by 6 citations

Experiments performed 24 experiments Detail

1 2D 1H-15N HSQC